Mahesh Ukade
Scientist
Nashik,MH
Summary
Knowledgeable laboratory professional skilled in all facets cancer research and clinical laboratory testing. Familiar with CAP,ISO and CLIA guidelines. Quality-driven team player with accuracy-focused and efficient approach, offering >3 years of industry expertise.
Overview
3
3
years of professional experience
6
6
years of post-secondary education
Work History
Scientist
Datar Cancer Genetics
Nashik, Maharashtra
09.2018 - Current
- Designs experimental plans with guidance and executes experiments, analyzes data, generates laboratory protocols.
- Mammalian Cell line maintain SKBR-3,HELA,MCF-7,MDAMB-231,A549,OVCAR..etc.
- Expertise in cell based assay development.
- Independently design experiments and conduct basic troubleshooting.
- Cytotoxic And targeted drugs library handling and perform cytotoxic assays.Handling of more than 30 Anticancer drugs and targeted drugs library.(Cisplatin,5Flurouracil,Carboplatin,Paclitaxel Everolimus,Sirolimus Doxorubicin..etc.)
- Validation and Documentation of new protocols, optimization of reagent/antibody/kit.
- Different cancer cell detection by using cancer specific marker.Targeted pathway antibody assay perform mTOR,EGFR,VGFR.
- Chemo-sensitivity based assay on cancer Patient blood/Tissue cells by follow NCCN guidelines.
- Daily basis Isolation of cancer cells from Tissue/blood and performing Immunoassays and chemo- sensitivity
- Plan personnel capacity and equipment resources, experimental setup, analysis and coordination of process development
- Participate in process transfers and coordination of interfaces as well as reporting and troubleshooting
- Perform media and solution preparation, maintain cell lines, and conduct cellular based assays while utilizing aseptic techniques
- Human cell line development and maintaining
- Characterization of cells based on immunoassay
- Assays on Fluorescence based,Fluorometric assays
- Familiar with LIMS,Cellest software(cell countor),IMAGE J,STEVE and EVE(3D imaging)
- Isolation of DNA/RNA, various organelle of cell
- Biochemical test
- Microbial cell culture techniques
- Microbial handling and sterilization techniques.
- INSTRUMENTATION:
- SDS PAGE,Agarose Gel Electrophoresis
Spectrophotometric techniques - ELISA, Immuno electrophoresis techniques
Micropropagation of plants through different tissue culture techniques - Instrumentation:3D cell explorer Nanolive System: for high precision tomographic microscope for 3D live cell imaging
Incucyte S3 Imaging system: live-cell imaging and analysis platform
EVOS M7000 - Varioskan Lux multimode microplate reader: Multi-mode microplate reader for UV/Vis,Fluorescence and Phosphorescence.
- MACS Quant Tyto cell sorter Milteny
Gentle MACS Octo Dissociator Milteny
Education
BSc. - Biotechnology
KTHM College
Nashik 06.2013 - 04.2015
MSc - Biotechnology
KTHM College
NAshik 06.2016 - 05.2018
Higher secondary - HSC Science
Janta Vidyalayon Saykheda
Nashik 06.2011 - 05.2013
Skills
Has good planning and organizing skill with ability for problem solving
Accomplishments
- Publication
- Abstract PS4-36: Non-invasive evaluation of chemoresistance in breast cancers using circulating tumorassociated cells February 2021 DOI:10.1158/1538-7445.SABCS20-PS4-36
- Project-Title :Eco friendly chitosan:An efficient material for effluent and water treatment
Timeline
Scientist
Datar Cancer Genetics
09.2018 - Current
MSc - Biotechnology
KTHM College
06.2016 - 05.2018
BSc. - Biotechnology
KTHM College
06.2013 - 04.2015
Higher secondary - HSC Science
Janta Vidyalayon Saykheda
06.2011 - 05.2013
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