Mahesh Ukade
Scientist
Nashik,MH
Summary
Knowledgeable laboratory professional skilled in all facets cancer research and clinical laboratory testing. Familiar with CAP,ISO and CLIA guidelines. Quality-driven team player with accuracy-focused and efficient approach, offering >3 years of industry expertise.
Overview
3
3
years of professional experience
6
6
years of post-secondary education
Work History
Scientist
Datar Cancer Genetics
Nashik, Maharashtra
09.2018 - Current
- Designs experimental plans with guidance and executes experiments, analyzes data, generates laboratory protocols.
- Mammalian Cell line maintain SKBR-3,HELA,MCF-7,MDAMB-231,A549,OVCAR..etc.
- Expertise in cell based assay development.
- Independently design experiments and conduct basic troubleshooting.
- Cytotoxic And targeted drugs library handling and perform cytotoxic assays.Handling of more than 30 Anticancer drugs and targeted drugs library.(Cisplatin,5Flurouracil,Carboplatin,Paclitaxel Everolimus,Sirolimus Doxorubicin..etc.)
- Validation and Documentation of new protocols, optimization of reagent/antibody/kit.
- Different cancer cell detection by using cancer specific marker.Targeted pathway antibody assay perform mTOR,EGFR,VGFR.
- Chemo-sensitivity based assay on cancer Patient blood/Tissue cells by follow NCCN guidelines.
- Daily basis Isolation of cancer cells from Tissue/blood and performing Immunoassays and chemo- sensitivity
- Plan personnel capacity and equipment resources, experimental setup, analysis and coordination of process development
- Participate in process transfers and coordination of interfaces as well as reporting and troubleshooting
- Perform media and solution preparation, maintain cell lines, and conduct cellular based assays while utilizing aseptic techniques
- Human cell line development and maintaining
- Characterization of cells based on immunoassay
- Assays on Fluorescence based,Fluorometric assays
- Familiar with LIMS,Cellest software(cell countor),IMAGE J,STEVE and EVE(3D imaging)
- Isolation of DNA/RNA, various organelle of cell
- Biochemical test
- Microbial cell culture techniques
- Microbial handling and sterilization techniques.
- INSTRUMENTATION:
- SDS PAGE,Agarose Gel Electrophoresis
Spectrophotometric techniques - ELISA, Immuno electrophoresis techniques
Micropropagation of plants through different tissue culture techniques - Instrumentation:3D cell explorer Nanolive System: for high precision tomographic microscope for 3D live cell imaging
Incucyte S3 Imaging system: live-cell imaging and analysis platform
EVOS M7000 - Varioskan Lux multimode microplate reader: Multi-mode microplate reader for UV/Vis,Fluorescence and Phosphorescence.
- MACS Quant Tyto cell sorter Milteny
Gentle MACS Octo Dissociator Milteny
Education
BSc. - Biotechnology
KTHM College
Nashik 06.2013 - 04.2015
MSc - Biotechnology
KTHM College
NAshik 06.2016 - 05.2018
Higher secondary - HSC Science
Janta Vidyalayon Saykheda
Nashik 06.2011 - 05.2013
Skills
Has good planning and organizing skill with ability for problem solving
Reliable and interested in learn and apply new techniques
Communicate effectively with colleagues and Managers within laboratory
Is literate,numerate and computer literate
Willing to be involved in working with a team as well as independently
GLP compliance
LIMS labware,
Flurometric ,absorbance based assay
Accomplishments
- Publication
- Abstract PS4-36: Non-invasive evaluation of chemoresistance in breast cancers using circulating tumorassociated cells February 2021 DOI:10.1158/1538-7445.SABCS20-PS4-36
- Project-Title :Eco friendly chitosan:An efficient material for effluent and water treatment
Timeline
Scientist
Datar Cancer Genetics
09.2018 - Current
MSc - Biotechnology
KTHM College
06.2016 - 05.2018
BSc. - Biotechnology
KTHM College
06.2013 - 04.2015
Higher secondary - HSC Science
Janta Vidyalayon Saykheda
06.2011 - 05.2013