Aanand Sonawane
Chennai
Summary
- Permanent resident of USA
- PhD in Life Science( Molecular Oncology and Medical Biotechnology) with 11 years of postdoctoral research experience
- 8 years research experience in Human Solid Tumor and Hematological Malignancies
- More than 15 years of research experience in Cell and Molecular Biology field
- 4 years of postdoctoral research experience in HIV research field
- 10 years experience in designing, analyzing research data, writing articles for publication and presentation of data in meetings and conference
- Extensive experience in Human genetics and functional genomics, bioinformatics
- Experience in Managing research groups and projects.
- Extensive experience in planning and executing new research projects, experiment, preparing protocol, statistical analysis of data, interpretation of results and preparation of report
- 10 years of experience in collaborative research skills
- Excellent oral and written communication skills
Overview
18
18
years of professional experience
Work History
Research Scientist in Virology
George Washington University
11.2014 - Current
- Investigated a project focused on " effect of cocaine on HIV replication with reference to epigenetic modification in HIV promoter''
- Designed a project focused on "Inhibition of HIV replication by DNA PK inhibitor"
- Written and published a first author article in Virology journal
- Established viral reporter cell lines and produced lentivirus
- Isolated CD4T cells from Human Blood and performed surface marker staining for flow cytometry analysis
- Trained 2 postdoctoral fellow, 3 summer trainee and one lab technician
Research Associate in Immunology
St Jude Children's Research Hospital
09.2011 - 11.2014
- Investigated a project entitled “ Regulation of Myc induced nuclear antigen (MINA) by TGF-b and smad3 in naive CD4T cells of Th2 biased mice”
- Developed transgenic mice (Villin-Mina) by recombinant DNA technology
- Isolated and cultured naive CD4T cells from spleen and lymphnode of mouse
- Constructed various clones for conditional reporter mouse project using cre-loxp technology
- Used CLC software for sequence analysis of cloned plasmid
- Used Graph pad and Image J software for statistical and protein expression analysis
- Perfomed Real Time PCR for RNA quantitation
- Performed transcription factor binding site analysis in gene promoter using UCSF and Genomatix website
- Constructed GST-Mina clones to express and purify proteins in BL21 cells
- Presented weekly research reports to supervisors and peer groups
Postdoctoral Research Scientist in Oncology
Georgetown University
06.2009 - 09.2011
- Investigated and completed a project entitled “ Regulation of Mitochondrial Citrate Transporter by Mutant p53”In Breast Cancer
- Investigated "Interaction between mutant p53 and FOXO1 protein in breast cancer"
- Isolated and cultured Microvascular endothelial cells from rat lungs for various cellular assay
- Injected breast cancer cell lines to mice for tumor developement
- Constructed various mutant clones by site directed mutagenesis
- Analysed transcription factor binding sites in gene promoter by Genomatix software
- Used Graph pad prism software for statistical analysis
- Investigated DNA-Protein interaction by Nucleic Acid Pull down and chromatin immunoprecipitation assay (CHIP).
- Published three research papers
- Supervised one postdoc and one technician
Visiting Fellow (Molecular Virology)
National Institute Of Health
12.2005 - 05.2009
- Completed a training project focused on "Integration of Tat into HIV virion that enhances viral replication"
- Transfected plasmid to generate HIV virus
- Performed western blotting, luciferase assay and ELISA
- Maintain mammalian cell lines
- Performed in vitro transcription and translation assay
- Concentrated HIV virion by sucrose gradient
- Identified efficacious siRNAs for multiple target genes for in vivo studies
Education
PhD (Molecular Oncology) - Life Science
Utkal University
Bhubaneswar, Odisha2004
Master of Science - Zoology and Biochemistry
Berhampur University
Berhampur, Odisha1997
Bachelor of Science - Zoology
Berhampur University
Berhampur, Odisha1993
Skills
- Nucleic Acid and protein Isolation from Human solid tumor
- Southern and Western Blotting
- DNA Methylation
- PCR-SSCP
- Nucleic Acid Pull down
- RT-PCR
- Quantitative PCR
- In-vitro transcription
- In-vitro translation
- Gene Cloning
- Site Directed Mutagenesis
- Gel Shift Assay (EMSA)
- CHIP assay
- Histology
- Immunohistochemistry
- Immunofluorescence
- Co-immunoprecipitation
- GST-pull down assay
- Mammalian Cell culture
- Isolation of primary cells from tissue and culture
- Generation of stable cell lines
- Transfection
- Transduction
- Mouse Tumor Model
- Cell Proliferation assay
- CD4T cell isolation and culture
- Automax
- Luminex assay
- Cell surface marker staining
- Primer design
- Genomic software (CLC, Primer3, Genomatix)
- GRAPH PAD
- Lentivirus generation
- Lentivirus infection
- Statistical Data analysis
- Bioinformatics
Publications
- Claire G, Farley K, EL-Hage N, Aiamkitsumrit B, Fassnacht R, Kashanchi F, Ochem A, Simon GL, Karn J, Hauser KF, Tyagi M (2015), Cocaine Promotes both initiation and elongation phase of HIV-1 transcription by activating NF-kB and MSK1 and inducing selective epigenetic modifications at HIV-1 LTR, Virology, 483:185-202. (Impact factor : 3.278)
- Kolukula VK, Claire G, Wellstein A, Preet A, lacobazzi V, D’Orazi G, Albanese C, Palmieri F, Avantaggiati ML (2014), SLC25A1, or CIC, is a novel transcriptional target of mutant p53 and a negative tumor prognostic marker, ONCOTARGET, 5(5):1212-25.(Impact factor: 6.63)
- Kirilyuk A, Simoji M, Catania J, Claire G, pattabiraman N, Albanese C, Giordano A, Mocchetti I,Toretsky JA, Uversky VN, Avantaggiati ML (2012), An intrinsically disordered region of acetyltransferase p300 with similarity to prion like domains plays a role in aggregation, PLOS ONE, 7(11):e48243. (Impact factor: 4.1)
- Kandasamy K, Claire G, Parthasarathi K (2012), Real time imaging reveals endothelium mediated leukocyte retention in LPS treated lung microvessel, Microvascular Research, 83(3):323-31. (Impact factor: 2.929)
- Claire G, Jena RK (2011), Clinical Significance of p53 and Bcl-2 in Acute myeloid Leukemia Patients of Eastern India, Hematology Report, Oct 19; 3(3):e28.
- Perez RE, Knights CD, Claire G, Catania J, Kolukula VK, Stoler D, Graessmann A, Ogryzko V, Pishvaian M, Albanese C, Avantaggiati ML (2010), Restoration of DNA binding and growth suppressive activity of mutant forms of p53 via a PCAF mediated acetylation pathway, Journal of cellular Physiology, 225(2):394-405. (Impact factor: 4.218)
- Claire G, Wang DF, Chen Claudia B, Victor B Zhurkin, Rodney E Harrington, Ettore Appella, Gorden L Hager, Akhilesh K Nagaich (2010), p53 Binding to Nucleosomal DNA Depends on the Rotational positioning of DNA response element, Journal of Biological Chemistry (JBC), 285(2): 1321-1332. (Impact factor: 4.77)
- Claire G, Das BR (2005), Alteration of p73 in denovo pediatric Acute Lymphoblastic Leukemia, Biochemical and Biophysical Research Communication, 327(3): 750-5.(Impact factor: 2.4)
- Claire G, Jena RK (2005). Significance of intracellular Arsenic trioxide for Therapeutic response in Acute promylocytic leukemia, American Journal of Hematology, 78 (2): 113-6. (Impact factor: 4.138)
- Claire G, Nayak B, Patnaik S, Parija T, Das BR (2002). Rearrangement of p53 gene with overexpressed p53 protein in primary cervical cancer. Oncology Report, 9: 433-437.(Impact factor: 2.297)
- Claire G, Das BR (2002).Prognostic significanceof p53 and Bcl-2 in lymphoblastic leukemia. Oncology Report, 9: 1391-1398. (Impact factor: 2.297)
Timeline
Research Scientist in Virology
George Washington University
11.2014 - Current
Research Associate in Immunology
St Jude Children's Research Hospital
09.2011 - 11.2014
Postdoctoral Research Scientist in Oncology
Georgetown University
06.2009 - 09.2011
Visiting Fellow (Molecular Virology)
National Institute Of Health
12.2005 - 05.2009
PhD (Molecular Oncology) - Life Science
Utkal University
Master of Science - Zoology and Biochemistry
Berhampur University
Bachelor of Science - Zoology
Berhampur University
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