Amarnath Pal

Key Deliverables:

• Set up equipment, organized inventory and maintained facilities.
• Leveraged interpersonal and communication skills to mentor PhD, graduate and undergraduate students.
• Liaised with supervisor to review grants and manuscripts to develop potential publications.
• Leveraged highly effective communication and active listening skills to work effectively scientists of diverse backgrounds and accomplish common research goals.
• Draft a comprehensive manuscript, 'Chromosomal Instability (CIN) on Glioblastoma Progression and Its Translational Opportunity.' (The manuscript is accepted in Advanced Science; IF: 14, 2026.)
• Studying the cellular intake of nanoparticles and their cytotoxic effect for constructing a nanoparticle-mediated drug delivery system in glioblastoma patients (a collaborative project, and the manuscript is under communication in a internationally reputed journal).
• Investigating growth factor–driven signaling pathways responsible for the epigenetic alteration of tumor suppressor genes and metabolic pathways in order to broaden the opportunity for developing promising targeted therapeutics in glioblastoma (the work is in progress).
• Preparation of in-house recombinant DNA for expressing wild-type and mutant proteins, facilitating single-molecule studies for understanding the impact of drug molecules on protein kinetics. (Serving as a team leader in the field of genetic engineering.)
• Prepared a high-efficiency protocol to prepare chemically competent bacterial cells (DH5-α and BL21) for one-shot transformation of difficult-to-transform vectors, including lentivectors.
• Established ISO 7 and ISO 8 standard human cell culture laboratory, and training and mentoring researchers in the field of cell and molecular biology, and advanced microscopy.
• 2D and 3D cell culture in glioblastoma, human breast cancer, and HEK293T cell lines.
• Providing advanced training in preparing in-house lentivirus-driven stable cell line preparation using vector design.
• Maintaining and monitoring laboratory logbooks dedicated to laboratory equipment and consumables.
• Expert in writing scientific reports and presenting scientific data in research groups and conferences.

Key Deliverables:

• Manufacturing diagnostic product line.
• Established strong relationships with vendors, negotiating favorable contracts for materials and services required in R&D efforts.
• Maintained open communication channels between R&D department staff and senior management, ensuring full transparency and alignment of goals.
• Improved prototype success rate with rigorous testing protocols and data analysis.
• Reduced production costs, optimizing material sourcing and manufacturing processes.
• Coordinated with marketing teams to develop effective product promotion strategies based on research findings.
• Streamlined R&D processes for faster project completion and improved efficiency.
• Mentored junior team members to support their professional growth and enhance overall team performance.
• Evaluated existing procedures and made proactive adjustments to meet changing demands.
• Successfully formulated rapid tests for HCG, S. typhi, Dengue, HBsAg, and antigen/antibody detection in patient samples.
• Developing a prototype for a fluorescence-based detection system.
• Performing stability tests for a sensitive diagnostic product line.

Key Deliverables:

• Working on CRISPR/Cas-driven gene regulation at the transcriptional level in mammalian cells.
• Validating in-house developed drug molecules targeting CRISPR/Cas activity.
• Developing strategies in order to make CRISPR/Cas as a therapeutic regimen for cancer invasion.
• Formulating novel, rapid tests for detecting cancer biomarkers and COVID in patient samples.
• Extensive experience in setting up and operating a tissue culture laboratory.
• Directing the purchase team to procure raw materials for sustainable research.
• Provide training and assist research scholars in setting up and executing experiments in core cell and molecular biology.
• Maintaining laboratory equipment and inventory.
• Participated in lab meetings and project presentations.

Key Deliverables:

• Conducted training sessions for staff members on the latest application technologies, enhancing overall team expertise.
• Coordinated collaborations with external partners, leveraging complementary expertise toward successful outcomes in joint projects.
• Updated technical documentation, product specifications, and technical training materials.
• Tested functional compliance of company products.
• Developed a highly sensitive rapid assay based on gold nanoparticles and latex beads-mediated detection of COVID-19 in patient samples at Imperial Life Sciences.
• Designing experiment plans and manufacturing diagnostic kits.
• Support cell and molecular biology, gene editing, and mutation detection research works at academic and industrial platforms across India.
• On-field and off-field visits for scientific meetings, demonstrating experiments, and troubleshooting scientific issues against any products provided by Principals.
• Reviewing universal selling points of a product, and designing promising content for product promotion.
• Provide guidance to operate Electroporator to our Indian customers.
• Delivering scientific presentations to my team in order to keep updated regarding new products for high-end research work.
• Supervise technical officers, and provide technical support to our team whenever required.

Key Deliverables:

• Organized research materials, maintaining a well-ordered workspace conducive to productivity.
• Managed laboratory equipment and resources, ensuring proper maintenance and optimal functionality.
• Processed xenograft tissues of breast cancer for assessing blood vessel density and immune cells in the tumor stroma through immunohistochemistry (IHC), and investigating the necrosis-inducing property of drugs in cancer tissues.
• Carried out experiments and research according to the protocols laid out by primary researchers.
• Actively involved in the process of conducting literature reviews and collecting and analyzing data.
• Provided ready access to all experimental data for the faculty researcher and/or supervisor.
• Created presentation slides and posters to help researchers present findings.
• Reviewed print and online resources to gather information.

Key Deliverables:

• Collaborated on interdisciplinary projects with other departments within the organization to achieve broader scientific goals.
• Operated analyzers to perform various biochemical tests and investigate the presence of tumor biomarkers in patient samples.
• Handled serum protein electrophoresis (SPE) for detecting multiple myeloma in patients.
• Developed and executed tests to detect disease, genetic disorders, or other abnormalities.
• Prepared reports and recommendations based on research outcomes.