Student Intern
Madras Diabetes Research Foundation
Chennai
05.2023
- Assisted in maintaining and culturing NRK and THP 1 cells, ensuring aseptic techniques and optimal growth conditions
- Carried out routine cell culture tasks including preparation of cell culture media, cell seeding, passaging, cryopreservation and thawing of cells
- Assisted in conducting cell-based assays involving ROS assay, TBARS assay, Bradford assay, NBT assay, Foam cell assay and cell viability assay
- Conducted Isolation of RNA using Phenol-Chloroform method and confirmed its purity using Nanodrop
- Performed SDS-PAGE and western blotting to analyse protein expression levels and validate experimental results
- Assisted in conducting RT-qPCR to quantify gene expression
- Participated in weekly journal club and engaged in discussion with senior researchers to explore different research findings.