Student Intern
Madras Diabetes Research Foundation
Chennai
05.2023 
- Assisted in maintaining and culturing NRK and THP 1 cells, ensuring aseptic techniques and optimal growth conditions
 - Carried out routine cell culture tasks including preparation of cell culture media, cell seeding, passaging, cryopreservation and thawing of cells
 - Assisted in conducting cell-based assays involving ROS assay, TBARS assay, Bradford assay, NBT assay, Foam cell assay and cell viability assay
 - Conducted Isolation of RNA using Phenol-Chloroform method and confirmed its purity using Nanodrop
 - Performed SDS-PAGE and western blotting to analyse protein expression levels and validate experimental results
 - Assisted in conducting RT-qPCR to quantify gene expression
 - Participated in weekly journal club and engaged in discussion with senior researchers to explore different research findings.
 
