Ankit Pratap Singh

CAR T cell-induced cytokine release syndrome is mediated by

macrophages and abated by IL-1 blockade

• Cytokine release syndrome (CRS), a systemic inflammatory response caused by cytokines released by infused CAR T cells can lead to widespread reversible organ dysfunction. CRS is the most common type of toxicity caused by CAR T cells.
• The exact mechanism of CRS remains to be better understood. Cytokines are released when interaction between tumor and immune effector cell occurs; and it can originate not only from the CART cell but also from host immune cells such as macrophages, which respond in part to CART activation.
• Medications. Cytokine reaction syndrome may also be induced by certain medications, such as the CD20 antibody rituximab and the CD19 CAR T cell tisagenlecleucel. The experimental drug TGN1412—also known as Theralizumab—caused extremely serious symptoms when given to six participants in a Phase I trial.

• JKG BIOSCIENCE PVT LTD. NOIDA
• Participated in a two-day workshop by JKG Bioscience Pvt limited.
• Acquired hands-on experience in DNA extraction and RFLP techniques.
• Applied practical knowledge, reinforcing precision in molecular biology procedures.
• Strengthened laboratory skills for future applications in biotechnological settings.