Arman Ahmad Ansari

• Optimised Synthesis Description: Conducted a multi-step synthesis of a novel ligand for MRI contrast agents, resulting in a high-quality product under expert supervision.

• Streamlined Analytical Processes: Utilised HPLC and LC-MS for reaction monitoring and product purification, ensuring accurate structure confirmation through NMR.

• Enhanced Reporting Techniques: Compiled comprehensive findings and compared results with related ligands, achieving the highest thesis grade.

• Determined ligand protonation constants and the stability of the Mn(II) complex via pH-potentiometric titration.

• Conducted quantitative and potency analysis of compounds in accordance with cGMP and GLP requirements.

• Performed potency testing via UV-Vis spectrophotometry and determined water content using Karl Fischer titration.

• Utilized HPLC for assay determination, optical purity analysis, and impurity profiling.

• Identified and quantified impurities using GC-MS to support product safety assessments.

• Adhered to SOPs, performed instrument setup, and prepared samples with precision.

• Operated an Agilent CE 7100 system with UV detection coupled to a Bruker MaXis II ESI-QTOF mass spectrometer for the analysis of biological and protein samples.

• Verified molecular formulas of compounds based on accurate mass, isotopic patterns, and [M+H]+ / [M+Na]+ adducts, and determined mass accuracy (ppm error).

• Investigation of bottom-up and top-down proteomics workflows, peptide fragmentation (b/y ions), and data analysis using Bruker Compass DataAnalysis under Professor Attila Gáspár.