Arya Pratap Rao

To secure a challenging role in an organization which offers me an opportunity to continuously learn and evolve myself, such that my growth is linked with that of the organization.

HPLC Techniques-

• HI-HPLC (Organic)
• RP-HPLC (Quantitation and Identity)
• SE-HPLC
• - IE-HPLC
• CEX –Hplc
• Protein A-HPLC
• N-Glycan Profiling by HPLC
• Peptide Mapping by RP-UPLC
• Sialic Acid Quantification

• RP-UPLC (Quantitation)
• Peptide Mapping by RP-UPLC
• CE SDS for impurity analysis of mAbs

• Quantitation of Residual Protein A by ELISA Kit
• Quantitation of Host Cell DNA
• Identification and Impurity analysis by SDS PAGE and blotting
• Protein Estimation by 280 nm
• Blotting (Western Blotting)
• Isolation and basic culture technique of Microbes
• Antibiotic sensitive test
• Preparation of Competent Cell (DH5α) (B121)
• Transformation of clone vector in E. coli
• Restriction Digestion
• Ligation reaction
• Protein Extraction and Purification
• Apperance,pH, Extractable Volume, Relative Density
• Affinity column Chromatography (NI-NTA)

• Calibration of HPLC
• Agilent module 1260,1100,1200, Infinity II , Shimadzu module,Waters HPLC and Thermo ultimate 3000
• Daily Calibration and verification
• Analytical Balance
• pH Meter
• Temperate Verification

• Participated in IQ,OQ and PQ Activity of Chromeleon software 6.8 and 7.2
• Participated in IQ,OQ and PQ Activity of Equipment like CZE and HPLC
• Excel Validation IQ,OQ and PQ Activity
• Performed IQ,OQ and PQ Activity in CSV

• Analytical Method Validation Protocol, Report and full Execution (Analysis) of N- Glycan.
• Analytical Method Validation Protocol, Report and full Execution (Analysis) of Polysorbate 20.
• Analytical Method Validation Protocol,Report and full Execution (Analysis) of Peptide Mapping.
• Analytical Method Validation Protocol, Report and full Execution (Analysis) of CZE.
• Excel Spread Sheet Validation
• Excel Spread Sheet Validation (IQ, OQ and PQ) for all Biological related analysis.

• Handling and Timely closing of Laboratory Incident
• Initiation and timely closing of Deviation and Investigation related activity.
• Initiation and timely closing of OOS and related Investigation activity.
• Initiation and timely closing of CAPA
• Preparation of Specification, STP, DRS, MOA.

• HPLC (Agilent Mod. ,1100,1200,1260 infinity II ,Shimadzu,Thermo Ulitmate 3000)
• Waters HPLC
• UV Spectrophotometer(Make Shimadzu and Agilent)
• Multimode plate reader (Make Perkin)
• Capillary Electrophoresis(Beckman Coulter)
• Dionex (Hplc for IE-Hplc)

Father name : Shri. Sanjay Rao

Avocations : Playing cricket, Listening music

Date of Birth : 30 Nov 1995.

Marital Status : Married

Language known : English, Hindi,

Permanent Address ; Vill- Pipra Bujurg Post-Harpurmafi Dist-Kushinagar City- Gorakhpur UP