BHABESH BORPHUKAN

Dedicated research fellow with a proven track record in conducting insightful research and analysis in plant molecular biology and gene editing. Experienced in data generation and interpretation, trend identification, and providing strategic recommendations. Known for strong analytical skills, meticulous attention to detail, and a passion for driving innovation and excellence in research initiatives.

• All India 49th ranked in ICAR-JRF scholarship 2011 (ICAR-JRF for M. Sc.)
• All India 60th ranked in JNU-CBEE 2011 (Jawaharlal Nehru University-Combined Biotechnology Entrance Examination)
• DBT-JRF fellowship-2014 awarded by DBT, Govt. of India
• Qualified for ASRB-NET 2015 (Agricultural Scientist Recruitment Board-National Eligibility Test for Lectureship)

Transgenic Manipulation of Glutamine Synthetase: A Target with Untapped Potential in Various Aspects of Crop Improvement, Biotechnology's of Crop Improvement, Volume 2, Gosal S., Wani S., Springer

• Crop Improvement Through CRISPR/Cas9: The Next Gen-Breeding Tool for Tailoring the Crop Genome and the Future of Tailored/Genome Edited Crops
• New insight into freezing tolerance: emerging concepts in low-temperature stress memory and regulatory networks in plants
• Characterization of the SmsHSP24.1 Promoter: Unraveling Abiotic Stress Signaling Mechanisms in Eggplant for Genetic Enhancement

• Solanum melongena mitochondrial small heat shock protein 24.1 mRNA, MF579857.1
• Heat inducible Promoter of Solanum melongena SmHSP24.1 gene, BankIt2754018/SmsHSP/OR703026

• To identify and characterize the CAMTA and CBF genes in pennycress and determine their expression patterns in response to cold stress.
• To determine the functional roles of CAMTAs and CBFs in pennycress cold tolerance by overexpressing or knockout these genes and measuring their effects on gene expression, physiological responses, and survival rates under freezing conditions.
• To identify the upstream/downstream target genes and pathways involved in cold acclimation and freezing tolerance in pennycress.
• Lipid profile analysis in fae1 and fad2 gene edited pennycress lines to understand their role in freezing stress tolerance in winter pennycress lines.

International Conference on Biotechnology and Human Welfare, 30 min, India, 2013, Ab initio Candidate Gene Prediction in Quantitative Trait Loci in Plants. 

3rd International Plant Physiology Congress, India, 2015, 

4th International Plant Physiology Congress, India, 2018,

International Conference on Plant Science Research, 30 min (Talk), USA, 2019, 21st Century Challenges to Food Security: An Attempt to Increase Crop Production through Transgenic and Genome Edited Approaches.

Sensing and signaling in pant stress response, India-EMBO symposium, India, 2019, 

MPS HATCH umbrella retreat, Pullman, USA, 2023, Pennycress: A fascinating story of plant adaptation to extreme cold temperature. 

2nd and 3rd IPreP Meeting, Illinois and Minnesota, 2022, 2023

Washington State University, Department of Crop and Soil Sciences, Pullman-99163

bhabesh.borphukan@gmail.com

bhabesh.borphukan@wsu.edu

orcid.org/0000-0002-2932-9390

• Enhance gene knock-in via hijacking HDR pathway by using virus based autonomously replicating donor template in rice, Plant Biotechnology, (Under review)
• Development and assessment of unique dual herbicide tolerance rice system through transgene stacking, GM crops and Food, (Submitted)

• M. Sc. Project: Evaluation of mini core germplasm of rabi sorghum for charcoal rot resistance and yield component traits, expression analysis of selected R-genes during charcoal rot disease incidence (Project funded by ICAR, Supervision: Dr. B. Fakrudin), Evaluated a highly diverse 242 minicore set of sorghum germplasm to find out new source of resistance against necrotrophic fungi M. phaseolina through a combined approach of phenotypic, biochemical and SSR markers screening., Expression profiling of a set of candidate R- genes using Real-time PCR technique.
• PhD project: Homologous recombination mediated genome engineering using CRISPR/Cas9 system rice (Project funded by ICAR, Supervision: Dr. M. K. Reddy), Developed autonomous replication system for delivery of homologous repair template in Indica rice varieties., Developing gateway compatible gRNA cloning constructs using rice specific U6/U3 promoters, Developed rice transformation and regeneration protocol in commercially popular Indica rice variety MTU1010, Precise base change in two rice native genes, EPSPS and ALS to developed herbicide resistant rice using CRISPR/Cas9 system.
• RA Project: Functional characterization of mitochondrial small heat shock protein (sHSP) genes for abiotic stress tolerance in eggplant (Indo-Bangladesh, Supervision: Dr. M. K. Reddy), Identification, cloning and biochemical characterization of Mitochondria localized nuclear small heat shock protein (SmsHSP24.1) in eggplant., Developed protocol for transformation and regeneration in eggplant variety., RNAseq data analysis in SmsHSP24.1-overexpressed eggplant lines., Promoter analysis using Promoter: GUS fusion constructs
• Postdoctoral Research Associate, ICGEB, Crop Improvement group, India, (November 2019 to August 2020), Title: Multiple gene knock-out for developing disease resistant rice and yield improvement (DBT/Govt. of India), Supervisor: Dr. M. K. Reddy, Developing multi-gene target gRNA construct, Rice transformation, target mutation identification and physiological analysis of edited lines., LC-MS analysis of phytohormones in edited lines., RNAseq analysis in edited lines.

• Date of Birth: 03/31/87
• Nationality: Indian
• Marital Status: Single

• Research papers, In Silico Analysis Identified bZIP Transcription Factors Under Abiotic Stress in Alfalfa (Medicago sativa L.), BMC Genomics, Accepted, Version 1, https://doi.org/10.21203/rs.3.rs-2728610/v1
• Research papers, Mitochondria-targeted SmsHSP24.1 overexpression stimulates seedling vigour and stress tolerance by multi-pathway transcriptome-reprogramming, Front Plant Sci., 10.3389/fpls.2021.741898, Khatun M. M
• Borphukan B.
• Alam, I., Reddy, M.K., Salimullah, M.
• Equal contribution/contributory first author
• Research papers, An improved protocol for Agrobacterium mediated transformation for successful Genetic engineering in eggplant, Sci. Hortic., 293, 110716, 2022, Khatun M. M
• Research papers, Overexpression of improved EPSPS gene results in field level glyphosate tolerance and higher grain yield in rice, Plant Biotechnol J., 10.1111/pbi.13428, Achary, V. Mohan, Sheri, Vijay, Mrinalini, M., Varakumar, P., Borphukan, B.
• Research papers, Concurrent overexpression of OsGS1;1 and OsGS2 genes in transgenic rice (Oryza sativa L.): impact on tolerance to abiotic stresses, Front. Plant Sci., 9, 786, 2018, James D., Borphukan B.
• Research papers, Co‐expression of P173S mutant rice EPSPS and igrA genes results in higher glyphosate tolerance in transgenic rice, Front Plant Sci, 13, 144, 2018, Fartyal, D., Agarwal, A., James, D., Borphukan, B.
• Research papers, Developing dual herbicide tolerant transgenic rice plants for sustainable weed management, Scientific reports, 8, 1, 1-12, 2018, Fartyal, D., Agarwal, A., James, D., Borphukan, B.
• Research papers, Characterization of phoA, a Bacterial Alkaline Phosphatase for Phi Use Efficiency in Rice Plant, Front. Plant Sci., https://doi.org/10.3389/fpls.2019.00037, Babu Ram, Fartyal, D., Vijay Sheri, Varakumar P., Borphukan B.
• Research papers, Association analysis of charcoal rot disease component traits in sorghum minicore germplasm with EST-SSR markers, Indian Journal of Genetics and Plant Breeding, 77, 1, 74-82, 2017, Kumar, B.N.H., Bhat, S., Borphukan, B., Fakrudin, B.
• Research papers, Sequence homology-based classification and characterization of microRNA families from selected plant species, J. Cell Tissue Research, 14, 1, 4089-4098, 2014, Mahale, B. M., Gund, S. V., Daspute, A., Borphukan, B.
• Research papers, Evaluation of mini core germplasm of rabi sorghum for charcoal rot resistance and yield component traits, expression analysis of selected R-genes during charcoal rot disease incidence, Karnataka J. Agric. Sci., 27, 2, 2014, Borphukan, B., Fakrudin, B.

Hereby, I do declare that all the statements made in this relevance are correct and complete to the best of my knowledge and belief.