Chitra Manoharan
Bengaluru,KA
Summary
Biotechnology professional with 8 years of extensive experience in Recombinant Protein Expression utilizing diverse expression systems including BEVS, Pichia pastoris, Escherichia coli, and Cell-free systems.
Proven expertise in developing and expressing Silk fusion proteins in transgenic silkworms. Skilled in protein extraction, purification, and characterization.
Proficient in molecular biology techniques such as nucleic acid isolation, PCR, vector construction, and DNA cloning. Adept at culturing primary cells (Human dermal fibroblasts and chicken primordial germ cells) and secondary cells (Bm, Sf9/Sf21, HeLa, and HEK293t cells).
Specialized in establishing project objectives, developing experimental plan and effectively prioritizing tasks to meet time sensitive delivery goals.
Displays strong written and oral communication skills to present complex analyses effectively.
Overview
9
9
years of professional experience
Work History
Senior Research Associate
Loopworm Private Limited
Bengaluru
07.2023 - Current
- Designed transposase-based vector for recombinant protein production in transgenic silkworm and cell lines.
- Maintained B.mori ovarian, Sf9, and Sf21 cell lines.
- Conducted BmNPV infection in cell lines and silkworms.
- Performed transfection in cell lines and silkworms.
- Propagated vo, v1, v2 viral generations and quantified viral titers.
- Characterized recombinant proteins using SDS-PAGE and western blot.
- Trained junior research assistants in cell culture techniques.
- Implemented quality control measures for experimental consistency.
Project Associate
ICAR-National Institute of Animal Nutrition and Physiology
Bengaluru
01.2023 - 06.2023
- Developed germline transgenic chickens using lentiviral vectors.
- Optimized microinjection procedures for LIF gene delivery.
- Maintained HEK cell lines and conducted lentiviral particle transfection.
- Screened transgenic chicks by PCR from gDNA isolated from blood.
- Cultured and characterized chicken primordial germ cells (cPGCs).
- Conducted in ovo injections for targeted delivery of transduced cPGCs.
Senior and junior research fellow
Seri-Biotech research laboratory
Bengaluru
09.2015 - 11.2019
- Recombinant expression of Fibroin–Cecropin B fusion protein in Pichia pastoris and Escherichia coli.
- Designed and constructed expression vectors.
- Extracted and Ni-IMAC purified recombinant proteins.
- Characterized proteins using SDS-PAGE, western blot, and MALDI-TOF.
- Tested antibacterial and wound healing properties of recombinant fusion proteins in HADF cells and rat.
- Developed transgenic silkworm spinning Fibroin–Cecropin B fusion protein by microinjection and electroporation.
- Stabilized transgenic lines and tested antibacterial activity from transgenic cocoons.
Education
Ph.D. - Biotechnology
Jain University
Bengaluru, IndiaMaster of Technology (M.Tech) - Biotechnology
M.S.Ramaiah Institute of Technology
Bengaluru, India09.2015
Bachelor of Engineering (BE), - Biotechnology
New Horizon College of Engineering
Bengaluru, India07.2010
Skills
- Molecular Biology: DNA/RNA extraction, cDNA synthesis, Primer Design, PCR, Vector construction, Transformation, Transfection, qPCR
- Protein Purification: IMAC, Ammonium Sulphate, Ultrafiltration, SDS-PAGE, Western Blot, Dialysis, Protein Quantification, Lyophilization
- Antimicrobial Assay: Kirby-Bauer method, Minimum Inhibitory Concentration
- Cell Culture: Maintenance and propagation, Passaging, Seeding, Cryopreservation, Lipofection, Viral Transduction, Cell Counting
Timeline
Senior Research Associate
Loopworm Private Limited
07.2023 - Current
Project Associate
ICAR-National Institute of Animal Nutrition and Physiology
01.2023 - 06.2023
Senior and junior research fellow
Seri-Biotech research laboratory
09.2015 - 11.2019
Ph.D. - Biotechnology
Jain University
Master of Technology (M.Tech) - Biotechnology
M.S.Ramaiah Institute of Technology
Bachelor of Engineering (BE), - Biotechnology
New Horizon College of Engineering