Summary
Overview
Work History
Education
Skills
Accomplishments
Affiliations
Certifications And Achievements
Publication
Languages
References
Timeline
Generic
Darshan S

Darshan S

Bangalore

Summary

Biochemistry postgraduate with specialization in molecular biology, enzymology, and biochemical techniques. Proven expertise in experimental design and application of spectroscopy and chromatography for complex biological investigations. Enhanced research accuracy and efficiency through optimized experimental protocols. Strong capabilities in data analysis and scientific communication.

Overview

1
1
year of professional experience

Work History

Master Thesis

Reva University
Bengaluru
08.2021 - 05.2022

Alpha-amylase inhibitory activity of Clerodendrum infortunatum plant as a natural source for diabetes management.

  • Assessed leaf extracts to determine potential as alternative diabetes treatment.
  • Evaluated phytochemicals in Thin Layer Chromatography (TLC) purified bands from ethyl acetate leaf extract using Liquid Chromatography-Mass Spectrometry (LC-MS) analysis.
  • Analyzed enzyme inhibition rates of leaf extracts through biochemical assays with UV-Vis Spectrophotometer.
  • Conducted statistical analysis on leaf extracts to calculate half-maximal inhibitory concentration (IC50).

Education

Master of Science - Biochemistry

REVA University
Bengaluru, India
11-2022

Bachelor of Science - Physics, Chemistry, Mathematics

St Jospeh’s College (Autonomous)
Bengaluru, India
10-2020

Skills

  • Alpha-amylase inhibition assay
  • Plant extract preparation
  • Agarose gel electrophoresis
  • Liquid Chromatography-Mass Spectrometry (LC-MS)
  • High-Performance Liquid Chromatography (HPLC)
  • Biochemical assays
  • UV-Vis spectrophotometer
  • RT-PCR
  • ELISA
  • SDS-PAGE
  • Media preparation
  • DNA/RNA isolation
  • Enzymatic assays
  • GraphPad Prism
  • MS Excel
  • MS PowerPoint
  • MS Word

Accomplishments

Orange Health Labs, July 2022 – April 2023, Bangalore

  • Worked in a diagnostic center as a junior lab technician, where my role involved testing and reporting serum and blood samples
  • Handled and performed QC and maintenance for machines that belonged to the Biochemistry (Vitros 5600), Haematology (Sysmex H8, H9), Clinical Pathology (AVE-772), Serology (Yumizen H550), and Immunology (Mini Vidas) departments
  • Used LIS software to track and manage over 200 patient samples per day

Affiliations

Hands-on training in an advanced analytical and molecular-based environment, and food testing, September 2021 – October 2021

Supervisor – Dr. V. A. Shanmuga Selvan, M/s NAWaL Analytical Laboratories, Hosur

  • Training is given for handling instruments like LC-MS, GC-MS, HPLC, RT-PCR analysis, DNA, RNA extraction, and purification
  • Estimation of different parameters for testing food and water samples
  • Media prep of SCDA, enumeration of bacteria (finger dab method), gram staining, diff methods of streaking

Certifications And Achievements

  • Scientific writing course, BioLim Centre for Science and Technology, Chennai, 2021
  • Workshop on reverse phase high performance liquid chromatography for determination of organic acid and vitamin C in fruit juices on 2nd and 3rd December 2021, Reva University, Bangalore
  • Volunteered for National Science Day events, held on March 9th and 10th, 2022, at Reva University

Publication

  • Shetty, D., & Kushwaha, R. K. (2024). Alpha-amylase inhibitory activity of Clerodendrum infortunatum plant as a natural source for diabetes management, The Indian Journal of Nutrition and Dietetics, 61(4), 417–428, https://doi.org/10.21048/IJND.2024.61.4.40566

Languages

English
Advanced (C1)
C1
Hindi
Advanced (C1)
C1

References

References available upon request.

Timeline

Master Thesis

Reva University
08.2021 - 05.2022

Master of Science - Biochemistry

REVA University

Bachelor of Science - Physics, Chemistry, Mathematics

St Jospeh’s College (Autonomous)
Darshan S