DEVI DEEKSHITHA PAPAREDDY

Results-oriented MSc Biotechnology candidate (Hyderabad Central University, DBT Fellow) with advanced expertise in molecular cloning, protein expression, and analytical biochemistry. Proven track record in independent research, troubleshooting, and collaborative problem-solving. Recognized for academic excellence (AIR 43 GAT-B, State 2nd AP PGCET) and innovation. Eager to contribute to cutting-edge R&D teams in the biotechnology and pharmaceutical industries.

• All India Rank 43 – Graduate Aptitude Test in Biotechnology (GAT-B)
• State 2nd Rank – AP PGCET
• Best Innovation Award – 3rd Annual Startup Jatara, AIC-SKU (2022)

• English
• Hindi
• Telugu

• Art & Craft
• Reading Books

Molecular biology and genetic engineering

• PCR, RT-PCR, nested PCR, RACE PCR, TA cloning, site-directed mutagenesis
• Plasmid construction, transformation, transduction, and conjugation
• Protein overexpression (bacterial systems), colony PCR, sequencing analysis

Protein biochemistry and analytical techniques

• Affinity, ion exchange, and size-exclusion chromatography
• SDS-PAGE, native PAGE, 2D electrophoresis, zymography, CBB/silver staining
• Protein quantification (BCA, Lowry), enzyme kinetics, ELISA, Western, Southern, Dot, and Dot blotting
• CD and fluorescence spectroscopy

Cell and microbial techniques

• Bacterial and fungal culture, plant tissue culture, and protoplast isolation
• Antibody generation, immunostaining, and agglutination assays

Bioinformatics and data analysis

• BLAST, SWISS-PROT, Primer3, PyMol, SnapGene, NEB cutter, CLUSTAL X/W, Oligo Calc
• Sequencing data analysis, protein structure visualization

Laboratory instrumentation

• UV-visible spectrophotometry, qRT-PCR, ultracentrifuge, sonicator, Nanodrop, protein crystallizer, lyophilizer

• Strong analytical and problem-solving abilities
• Excellent communication and scientific writing skills
• Ability to meet deadlines and work under pressure
• Self-motivated, adaptable, and collaborative team player

Master's thesis: Cloning, overexpression, and characterization of Bowman-Birk inhibitor from Vigna mungo

• Isolated high-quality RNA and performed cDNA synthesis using RT-PCR
• Designed ORF-specific primers and optimized PCR conditions
• Engineered recombinant constructs using double digestion, ligation, and transformation
• Verified cloning by colony PCR, restriction digestion, and sequencing
• Expressed target protein in bacterial hosts, optimized induction (IPTG, temperature)
• Purified protein using affinity chromatography, validated by SDS-PAGE, and Western blot
• Conducted in vitro enzyme inhibition and insecticidal assays on Lepidopteran pests
• Characterized protein structure using CD spectroscopy and functional analysis