Summary
Overview
Work History
Education
Skills
Achievements
Languages
References
Conference And Workshop
Research Projects And Internships
Timeline
Generic

Dipanwita Paul

Kolkata

Summary

I recently graduated with a Master’s in Biological Sciences from the Indian Institute of Science Education and Research (IISER), Thiruvananthapuram, and hold a dual degree in B.Sc. and B.Ed. from the Regional Institute of Education, Bhubaneswar. My research interests lie in host–pathogen interactions, molecular biology, and infectious disease biology.

Overview

3
3
years of professional experience

Work History

Major Thesis Project

IISER TVM
08.2024 - 07.2025
  • Optimized purification of recombinant Plasmodium falciparum CLK3 protein for downstream biochemical studies.
  • Cloned PfATPase4 gene into pET28a vector, enabling effective heterologous expression for further analysis.
  • Investigated Kelch13's role in artemisinin resistance in P. falciparum, contributing to understanding resistance mechanisms.
  • Shortlisted essential candidate genes in Toxoplasma gondii under DHA treatment conditions..

Summer Internship

Institute of Health Sciences, Presidency University
05.2024 - 07.2024
  • Isolated and characterized a novel RNA transcript from mouse brain tissue for qPCR analysis and cloning into the pET19 expression vector.
  • Investigated functional roles of lncRNAs and their regulatory effects post-CRISPR-Cas–mediated knockdown.
  • Identified human homologues of novel mRNA via comparative sequence analysis, contributing to understanding gene function.
  • Acquired hands-on experience in magnetic-associated cell sorting (MACS) and cryotomy techniques for cellular analysis.

Research Intern

IISER TVM
01.2024 - 04.2024
  • Investigated T-cell biology and key biomarker expression across activation and differentiation stages to deepen understanding of immune responses.
  • Acquired hands-on experience with flow cytometry (FACS) and antibody-based staining techniques to facilitate accurate cellular analysis.
  • Evaluated differential biomarker expression in T-cells under stimulatory and non-stimulatory conditions to assess immune activation profiles.

Research Intern

IISER TVM
08.2023 - 12.2023
  • Cultured and maintained bacterial cells to support research objectives.
  • Performed molecular biology techniques including transformation, gel electrophoresis, Western blotting, and NanoDrop-based nucleic acid quantification.
  • Learned primer designing and molecular cloning techniques.
  • Designed primers and performed molecular cloning to facilitate genetic studies.

Teaching Intern

Jawahar Navodaya Vidyalaya
09.2022 - 01.2023
  • Instructed 6-11 graders in basic sciences, fostering foundational knowledge and engagement.
  • Developed and executed learning activities inside and outside classroom, enhancing student participation and understanding.
  • Assisted teachers in delivering engaging lessons to diverse student groups.
  • Developed and implemented educational materials for classroom activities.
  • Supported classroom management to create a positive learning environment.

Education

M.Sc - Biological Sciences

IISER TVM
TVM, India

Int.B.Sc-B.Ed -

Regional Institute of Education
Bhubaneshwar, Odisha

Higher Secondary Education -

Sri Chaitanya Educational institutions
Vishakhapatnam, India

Skills

  • Bacterial cell culture
  • Mammalian Cell Culture
  • Cloning
  • Transfection
  • QPCR
  • PCR
  • Gel electrophoresis
  • SDS PAGE
  • Western blot
  • FACS analysis
  • Ni-NTA purification
  • Size Exclusion chromatography
  • Immunostaining
  • Immunofluorescence
  • Model Organism Techniques
  • Experimental Design
  • Protein assays

Achievements

  • Qualified NCERT CEE 2019
  • Qualified Central Teachers eligibility test 2023, Paper 1 and 2
  • Qualified IIT JAM biotechnology 2023
  • IISER TVM M.Sc Screening rest

Languages

  • English
  • Hindi
  • Bengali

References

  • Dr. Kamalakannan Vijayan, Assistant Professor, Indian Institute of Science Education and Research (IISER) TVM, kamalakannan.vijayan@iisertvm.ac.in, Thesis Advisor and mentor
  • Professor Nishant K.T, Professor, Indian Institute of Science Education and Research (IISER) TVM, nishantkt@iisertvm.ac.in

Conference And Workshop

  • Rhetor-Bio Symposium 2023, IISER Thiruvananthapuram, 08/25/23, 09/09/23
  • Frontier Symposium at IISER TVM 2024, IISER Thiruvananthapuram, 02/02/24, 02/04/24
  • Institute Colloquium IISER, IISER Thiruvananthapuram, 08/21/24
  • Rhetor-Bio Symposium 2024, IISER Thiruvananthapuram, 08/24/24, 09/01/24

Research Projects And Internships

  • Understanding anti malarial drug target proteins, Major thesis Project, Dr. Kamalakannan Vijayan, 08/01/24, 07/31/25, IISER TVM, Mammalian cell culture and infecting Hela cells with Toxoplasma gondii, Purification of PfCLK3 protein using Ni NTA, SEC useful as an antimalarial drug target., Cloning of ATPase4 gene in pET28a plasmid., Learnt about Kelch 13 gene and its role in Artemisinin drug treatment in malaria., Shortlisted candidate genes in Toxoplasma gondii based on their essentiality during DHA treatment.
  • Cloning and characterizing of novel Long non coding RNA, Summer Internship, Dr. Arpita Konar, 05/01/24, 07/31/24, Institute of Health Sciences, Presidency University, Isolated novel RNA from mouse brain for setting up qPCR and cloning in pET19 vector., Learnt about the function of Lnc RNA and their effects after knockdown with CRISPR-Cas., Characterised the novel mRNA and identified its human homologues., Learnt about Magnetic associated cell sorting and cryotomy.
  • Identification of Tcell Biomarkers, Semester through internship, Dr. Karthik Chandiran, 01/01/24, 04/30/24, IISER TVM, Learnt about Tcell and its various biomarkers expressed during its lifetime., Understood the techniques of FACS, antibody staining., Learnt about expression of biomarkers in Tcell under stimulatory and non-stimulatory conditions.
  • Foundational techniques in Molecular Biology, Semester through internship, Dr. N. Sadananda Singh, 08/01/23, 12/31/23, IISER TVM, Learnt Bacterial cell culture., Learnt techniques such as Transformation, Electrophoresis, Western Blotting, Nanodrop., Studied cytokinesis of Ecoli cells incorporated with CRISPR dCas9 and deactivated Ftsz gene., Learnt about primer designing and cloning techniques.
  • Teaching internship in Jawahar Navodaya Vidyalaya, 09/01/22, 01/31/23, Taught basic sciences to 6-11 graders., Implemented learning activities inside and outside the classroom.
  • Freelance content writer at little leap.co, 05/01/21, 10/31/21, Made activities for little kids for teaching moral and life values.

Timeline

Major Thesis Project

IISER TVM
08.2024 - 07.2025

Summer Internship

Institute of Health Sciences, Presidency University
05.2024 - 07.2024

Research Intern

IISER TVM
01.2024 - 04.2024

Research Intern

IISER TVM
08.2023 - 12.2023

Teaching Intern

Jawahar Navodaya Vidyalaya
09.2022 - 01.2023

M.Sc - Biological Sciences

IISER TVM

Int.B.Sc-B.Ed -

Regional Institute of Education

Higher Secondary Education -

Sri Chaitanya Educational institutions
Dipanwita Paul