Dona Ghosh
Baguihati
Summary
Experienced Annotator and QC expert with a strong background in optimising LLM-generated content for high-level Biology problem-solving, and proficient in LaTeX formatting. Currently a Project Trainee at the University of Calcutta, investigating the conserved role of ecto-hinge domain during rhizobial infection in root nodule symbiosis, with expertise in genomic DNA and RNA isolation, plasmid DNA isolation, RT-PCR, cDNA synthesis, primer designing, site-directed mutagenesis, sequencing PCR, promoter cloning, protein expression and purification. Previous experience includes training summer trainee and working as a Summer Intern at Saha Institute of Nuclear Physics on anti-cancer protein Azurin. Skilled in confocal microscopy, ELISA, Western Blotting and handling transgenic plants. Career goal: to contribute to advanced research in molecular biology and biotechnology.
Overview
2027
2027
years of professional experience
Work History
Biology Expert
Mindrift (Remote)
Kolkata
02.2026 - Current
- Contributing domain expertise to advanced biological AI training projects.
- Reviewing complex biological reasoning tasks for logical consistency.
Project Trainee, at the lab of
Prof. Maitree Dasgupta lab, University of Calcutta
Kolkata, West Bengal
07.2023 - Current
- Currently working on investigating the conserved role of ecto-hinge domain to release MLD (malactin like domain) during rhizobial infection in root nodule symbiosis.
- Generated mutation clones of two important residues of EctoD hinge region of SYMRK.
- Techniques performed and learnt: genomic DNA and RNA isolation from plant, Plasmid DNA isolation from bacteria, RT-PCR and cDNA synthesis, Primer designing and site- directed mutagenesis, sequencing PCR, promoter cloning, Restriction and Gateway cloning, His-tagged protein expression and purification using Ni-NTA column chromatography, Protein purification from inclusion bodies, and Confocal microscopy to study the infection thread morphology in Medicago.
Biology, English and Mathematics Teacher
01.2021 - Current
- Teach Biology, English and Mathematics to high school and undergrad students.
Bharatanatyam Dancer
Natya Kala Bhavan
Kolkata, West Bengal
01.2002 - Current
- Learning this classical dance form from Natya Kala Bhavan, Kolkata.
Biology Expert
TELUS AI (Remote)
Kolkata
12.2025 - 02.2026
- Annotated and validated life sciences datasets.
- Performed QC audits on AI-generated biology responses.
- Improved structured scientific reasoning in model outputs.
Biology Expert
Turing (Remote)
Kolkata
10.2025 - 01.2026
- Evaluated LLM-generated biology content for conceptual accuracy.
- Designed high-level biology reasoning problems.
- Provided expert-level domain validation feedback.
Annotator and QC expert
Soul Ai
Kolkata
06.2025 - 07.2025
- Optimized LLM-generated content for high-level Biology problem-solving.
- Served as a QC expert annotating LLM-generated prompts, emphasizing LaTeX formatting.
Summer Intern,
Saha Institute of Nuclear Physics
Kolkata, West Bengal
06.2022 - 07.2022
- Worked on Purification and characterization of novel potential anti-cancer protein Azurin.
- Purified the full-length wild-type protein Azurin via overexpressing BL21 E. coli system and measured the yield of the protein. The protein can be used further for structural biology studies.
- The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry, published in the scientific journal, Signal Transduction and Targeted Therapy, 2022, and presented in Journal Club at the University of Kalyani.
Participant
Indian Institute of Science Education and Research Kolkata
06.2022 - 06.2022
- Learned about Tissue harvesting, Tissue processing, Histology staining, Immunohistochemistry, Real-time RT-PCR, Flow cytometry, Fluorescent and in vivo imaging, Proteomics and metabolomics (Mass spectrometry, Liquid chromatography), Nuclear Magnetic Resonance, Surface plasmon resonance, Modeling, Isothermal titration calorimetry (ITC), Stopped flow kinetics, Fluorescence Spectroscopy.
- Genomic DNA and RNA isolation from plants, bacterial plasmid isolation, media and buffer preparations, Primer designing and introduction of site-directed mutagenesis, cloning, protein expression and purification, SDS-PAGE, qRT-PCR, handling transgenic plants, Confocal microscopy, ELISA, Western Blotting.
Trainer
University of Calcutta
Kolkata, West Bengal
- Trained two summer intern students at Prof. Maitreyee Dasgupta's Lab at University of Calcutta.
Education
M.Sc. - Molecular Biology and Biotechnology
University of Kalyani
2022
B.Sc. - Biochemistry
Calcutta University
2020
Skills
- Python
- R programming
- PyMOL
- Genomic DNA isolation
- RNA isolation
- Bacterial plasmid isolation
- Media preparation
- Buffer preparation
- Primer designing
- Site-directed mutagenesis
- Cloning
- Protein expression
- Protein purification
- SDS-PAGE
- QRT-PCR
- Handling transgenic plants
- Confocal microscopy
- ELISA
- Western Blotting
- LLM prompt annotation
- LaTex formatting of Texts
Publications
Title: SYMRK hinge adaptations enable Malectin-like Domain release for symbiont passage during rhizobia-legume symbiosis Authors: Dipanjan Chakrabarti, Anindita Paul, Firoz Molla, S. Bhattacharyya, Sagnik Das, Sampurna Chakraborty, Dona Ghosh, Alokmoy Biswas, Anindya Kundu, Senjuti Sinharoy, Maitrayee Das Gupta.
The Plant Cell, Volume 38, Issue 4, April 2026, koag053, DOI - https://doi.org/10.1093/plcell/koag053
Timeline
Biology Expert
Mindrift (Remote)
02.2026 - Current
Biology Expert
TELUS AI (Remote)
12.2025 - 02.2026
Biology Expert
Turing (Remote)
10.2025 - 01.2026
Annotator and QC expert
Soul Ai
06.2025 - 07.2025
Project Trainee, at the lab of
Prof. Maitree Dasgupta lab, University of Calcutta
07.2023 - Current
Summer Intern,
Saha Institute of Nuclear Physics
06.2022 - 07.2022
Participant
Indian Institute of Science Education and Research Kolkata
06.2022 - 06.2022
Biology, English and Mathematics Teacher
01.2021 - Current
Bharatanatyam Dancer
Natya Kala Bhavan
01.2002 - Current
Trainer
University of Calcutta
M.Sc. - Molecular Biology and Biotechnology
University of Kalyani
B.Sc. - Biochemistry
Calcutta University