Dr.Ravi Prakash

Applications professional with proven track record in application development, troubleshooting, and support. Known for delivering high-quality solutions and consistently meeting project deadlines. Strong focus on team collaboration and adaptability, ensuring reliable support and smooth operations. Expertise in technical analysis, problem-solving, and user training.

• Senior Research Fellowship, University Grants Commission, Government of India, 08/2017
• Junior Research Fellowship (JRF), University Grants Commission, Government of India, 08/2015
• Qualified Joint CSIR-UGC Test for Junior Research Fellowship (JRF) & Eligibility for Lectureship (NET), 08/2013

• Animal handling (Mice, Rat, Rabbit and hamster) and surgical mouse models
• Development of Osteoporosis model in rodents
• Drill hole injury model in rodents
• Anti-resorptive model in rodents
• Critical size defect model in Rabbit
• Development of Senescence model in rodents
• Neonatal culture for osteoblast and osteoclast
• Expertise in isolation and culturing different immune cells (Macrophage, T cells, splenocyte, dendritic (DCs), from Bone marrow and spleen)
• Molecular biology expertise (DNA/RNA purification, Knockdown (siRNA, ShRNA), Molecular Cloning, Real-time PCR, Immunohistochemistry (IHC), Immunofluorescence (IF), Immuno-precipitation (IP), Western Blotting, ELISA, Luciferase reporter assay, Cell proliferation assay, Flow cytometry)

• Application support for Bruker micro-CT
• Application support for Oroboros for metabolic analysis
• Application support for Stem cell technology

First Author Research Articles

• 1. Prakash R, Mishra T, Dev K, Sharma K, Kuldeep J, John AA, Tripathi A, Sharma C, Arya KR, Kumar B, Siddiqi MI, Tadigoppula N, Singh D. Phenanthrenoid Coelogin Isolated from Coelogyne cristata Exerts Osteoprotective Effect Through MAPK-Mitogen-Activated Protein Kinase Signaling Pathway. Calcif Tissue Int. 2021;109(1):32-43. Epub 2021/03/07. doi: 10.1007/s00223-021-00818-3. PubMed PMID: 33675370.
• 2. Imran Ahamad M*, Prakash R*, John AA, Wani Z, Yadav D, Bawankule DU, Luqman S, Khan F, Singh D, Gupta A. Induced osteoblast differentiation by amide derivatives of stilbene: The possible osteogenic agents. Bioorg Med Chem Lett. 2020;30(11):127138. Epub 2020/04/06. doi: 10.1016/j.bmcl.2020.127138. PubMed PMID: 32247734.(*Equal first authors)

• 3. Prakash R, John AA, Singh D. miR-409-5p negatively regulates Wnt/Beta catenin signaling pathway by targeting Lrp-8. J Cell Physiol. 2019;234(12):23507-17. Epub 2019/06/06. doi: 10.1002/jcp.28919. PubMed PMID: 31165485.

Co- author Research Articles

1. Tripathi A, Awasthi P, Rawat KS, Dwivedi AP, Singh KB, Sharma K, Prakash R, Singh D, Goel A. 3-PEP promotes bone regeneration by up regulating BCL-2 expression via ERK phosphorylation. J Endocrinol. 2022;254(1):51-64. Epub 2022/06/16. doi: 10.1530/JOE-21-0439. PubMed PMID: 35702893.

2. Sharma K, Kumar S, Prakash R, Khanka S, Mishra T, Rathur R, Biswas A, Verma SK, Bhatta RS, Narender T, Singh D. Chebulinic acid alleviates LPS-induced inflammatory bone loss by targeting the crosstalk between reactive oxygen species/NFkappaB signaling in osteoblast cells. Free Radic Biol Med. 2022;194:99-113. Epub 2022/11/25. doi: 10.1016/j.freeradbiomed.2022.11.026. PubMed PMID: 36423891.

3. Sharma K, Kumar A, Prakash R, Tripathi A, Singh R, Bajpai R, Sahasrabuddhe AA, Singh D, Narender T. Pyrazoline analogues: Design, synthesis, and evaluation of anti-osteoporosis activity. Bioorg Med Chem Lett. 2022;60:128585. Epub 2022/01/28. doi: 10.1016/j.bmcl.2022.128585. PubMed PMID: 35085723.

4. Sharma K, Awasthi P, Prakash R, Khanka S, Bajpai R, Sahasrabuddhe AA, Goel A, Singh D. Maintenance of increased bone mass after PTH withdrawal by sequential medicarpin treatment via augmentation of cAMP-PKA pathway. J Cell Biochem. 2022;123(11):1762-79. Epub 2022/08/13. doi: 10.1002/jcb.30313. PubMed PMID: 35959633.

5. Shweta, Gautam C, Dey KK, Ghosh M, Prakash R, Sharma K, Singh D. Influence of carbon nanotubes reinforcement on the structural feature and bioactivity of SiO2–Al2O3–MgO–K2CO3–CaO–MgF2 bioglass. Applied Physics A. 2021;127(7):545. doi: 10.1007/s00339-021-04708-1.

6. Gautam A, Gautam C, Mishra M, Sahu S, Nanda R, Kisan B, Gautam RK, Prakash R, Sharma K, Singh D, Gautam SS. Synthesis, structural, mechanical, and biological properties of HAp-ZrO2-hBN biocomposites for bone regeneration applications. Ceramics International. 2021;47(21):30203-20. doi: https://doi.org/10.1016/j.ceramint.2021.07.200.

7. Dev K, Begum J, Biswas A, Mir NA, Singh J, Prakash R, Sonowal J, Bharali K, Tomar S, Kant R, Ahlawat N. Hepatic transcriptome analysis reveals altered lipid metabolism and consequent health indices in chicken supplemented with dietary Bifidobacterium bifidum and mannan-oligosaccharides. Scientific Reports. 2021;11(1):17895. doi: 10.1038/s41598-021-97467-1.

8. John AA, Prakash R, Singh D. miR-487b-3p impairs osteoblastogenesis by targeting Notch-regulated ankyrin-repeat protein (Nrarp). J Endocrinol. 2019;241(3):249-63. Epub 2019/04/13. doi: 10.1530/JOE-19-0015. PubMed PMID: 30978699.

9. Maurya SW, Dev K, Prakash R, Aijaz, John A, Siddiqui IR, Singh D, Maurya R, editors. Design and Synthesis of Indolyl Chalcone Analogues and Evaluation of Their Osteogenic Activity2018.

10. Kureel J, John AA, Prakash R, Singh D. MiR 376c inhibits osteoblastogenesis by targeting Wnt3 and ARF-GEF-1 -facilitated augmentation of beta-catenin transactivation. J Cell Biochem. 2018;119(4):3293-303. Epub 2017/11/11. doi: 10.1002/jcb.26490. PubMed PMID: 29125885.

11. John AA, Prakash R, Kureel J, Singh D. Identification of novel microRNA inhibiting actin cytoskeletal rearrangement thereby suppressing osteoblast differentiation. J Mol Med (Berl). 2018;96(5):427-44. Epub 2018/03/11. doi: 10.1007/s00109-018-1624-y. PubMed PMID: 29523914.

12. Dixit M, Singh KB, Prakash R, Singh D. Functional block of IL-17 cytokine promotes bone healing by augmenting FOXO1 and ATF4 activity in cortical bone defect model. Osteoporos Int. 2017;28(7):2207-20. Epub 2017/03/28. doi: 10.1007/s00198-017-4012-5. PubMed PMID: 28341898.

13. Ahmad MI, Raghuvanshi DS, Singh S, John AA, Prakash R, Nainawat KS, Singh D, Tripathi S, Sharma A, Gupta A. Design and synthesis of 3-arylbenzopyran based non-steroidal vitamin-D3 mimics as osteogenic agents. MedChemComm. 2016;7(12):2381-94. doi: 10.1039/C6MD00469E.

Nationality: Indian

Language : Hindi &English

DOB         :  6 March 1990

Wife         : Dr. Ritika Prasad 

1. Presented Flash talk in 7th International symposium on “Current Trends in Drug Discovery Research” 20-23 February, 2019 at CSIR-Central Drug Research Institute, Lucknow.

2. One of the Webinar Titled  " POST COVID COMPLICATIONS ON HUMAN POPULATION" held on 16th June 2021 .  Department of Zoology M K M KANYA MAHAVIDHYALAYA

Poster Presentations

· Participated in Scientific and Technical Awareness Programme in Animal Experimentation (STAP), Human care and scientific Management of laboratory animal and basic animal technique held at CSIR-Central Drug Research Institute, Lucknow 19-21 August, 2015.

· Participated in 6th international symposium on “Current Trends in Drug Discovery & Research”. 25th -28th February, 2016 at CSIR-Central Drug Research Institute, Lucknow.

· Participated in confocal microscopy workshop from 8-9 November, 2016 organized by CSIR-Central Drug Research Institute, Lucknow.

· Participated in 13th National congress of Indian Society of Bone Mineral Research from 10th -12th November, 2017

· Attended & Presented a Poster in symposium on Molecular medicine for life style diseases: Emerging target and Approaches” from 20-21 November, 2017 at CSIRCentral Drug Research Institute, Lucknow.

· Presented Flash talk in 7th International symposium on “Current Trends in Drug Discovery Research” 20-23 February, 2019 at CSIR-Central Drug Research Institute, Lucknow.

· Presented Poster in 8th Annual Conference on “Navigating Translational Discoveries” from November 2-4, 2019 at CSIR-IICT Hyderabad.

· Participated in 2nd National Biomedical Research Competition on November 17th 2019 at PGIMER Chandigarh.

Dr. Divya Singh

Sr. Principal Scientist

Department of Endocrinology

CSIR-CDRI, Lucknow

Contact No. 9415596306

Email: divyacdri@gmail.com

Dr. T Narendra

Sr. Principal Scientist

Department of Medicinal Chemistry

CSIR-CDRI, Lucknow

Contact No.9415019297

Email: t_narendra@cdri.res.in

Dr.Vikash Vats

Clinical & Scientific Manager-Autoimmunity

Werfen Gurgaon 

Contact- 9871982288

wats@werfen.com