Summary
Overview
Work History
Education
Skills
Training and workshop
Research papers
Conferences
Academic chronicle
Areas Of Interest
Extracurricular Activities
Timeline
Generic

Garima Thapliyal

Dehradun

Summary

Driven by a passion for genetic improvement and conservation, I excelled at ICFRE-Forest Research Institute, spearheading SSR-based genetic evaluations of Ringal populations. My expertise in CRISPR-Cas9 and bioinformatics, coupled with a knack for innovative problem-solving, significantly contributed to the project's success, showcasing my technical proficiency and collaborative spirit.

Overview

7
7
years of professional experience

Work History

Juniour Project Fellow

ICFRE-Forest Research Institute
01.2018 - Current
  • Company Overview: ICFRE funded project “Characterization of the hill bamboo species (Ringal) of the North-Western Himalayas for their conservation and genetic improvement”
  • Independently carried out SSR based genetic evaluation of Ringal populations
  • ICFRE funded project “Characterization of the hill bamboo species (Ringal) of the North-Western Himalayas for their conservation and genetic improvement”

Education

PhD. - Forest Biotechnology

ICFRE-Forest Research Institute
Dehradun, Uttarakhand, India
05.2025

MSc. - Biotechnology

Graphic Era (Deemed to be University)
Dehradun, Uttarakhand, India
01.2017

B.Sc - Biotechnology

H.N.B Garhwal University
Srinagar, Uttarakhand, India

SSC -

I.C.S.E

HSC -

I.S.C

Skills

  • Gene editing
  • Virus induced gene silencing (VIGS)
  • CRISPR-Cas9
  • TA Cloning
  • Golden-gateway cloning
  • Klenow fragment-based cloning
  • RTBV vector
  • Construct development
  • Agrobacterium mediated transformation
  • Genomics
  • Transcriptomics
  • QPCR
  • Sequencing
  • Differential gene expression analysis
  • Bioinformatics
  • BLAST
  • Gene ontology (GO) analysis
  • CRISPOR
  • DECODR
  • Bioedit
  • SGNVIGS
  • Biogrid
  • STRING
  • MEGA
  • CLUSTALW
  • Statistics
  • Stat-ease
  • SPSS
  • Response surface methodology (RSM)
  • Graphpadprism
  • R
  • Plant stress physiology
  • Stress assays
  • NBT staining
  • DAB staining
  • Chlorophyll estimation
  • Nitrate reductase activity
  • Infrared gas analyzer
  • Li-COR
  • Ciras IRGA
  • Drought evaluation
  • Temperature evaluation
  • Chemical stress evaluation
  • Molecular techniques
  • DNA/RNA extraction
  • PCR
  • Gel electrophoresis
  • Tissue culture
  • Nodal explant propagation
  • Leaf explant propagation

Training and workshop

  • Workshop on advances in biotechnology and its applications in conservation biology, Department of Biotechnology Dolphin Institute in collaboration with Wildlife Institute of India and Zoological Survey of India.
  • International Bioinformatics workshop for cancer genomics, Decodelife
  • DST sponsored Hands on training programme on ‘Recent Technological Advancements in Horticulture and Forest Crops’, 12/23/22 - 12/30/22, Department of Biotechnology, College of Horticulture & Forestry, Nauni, Solan (HP), in collaboration with Sophisticated Analytical Instrumentation Facility, Panjab University, Chandigarh.

Research papers

  • Thapliyal, G., Vemanna, R. S., Pawar, P. M., Barthwal, S., Meena, R. K., Pandey, S., & Bhandari, M.S., First record of off-season flowering in Populus deltoides from India: paradigm of climate change indicator., International journal of biometeorology, https://doi.org/10.1007/s00484-020-01915, 3.4
  • Thapliyal, G., Bhandari, M. S., Vemanna, R. S., Pandey, S., Meena, R. K., & Barthwal, S., Engineering traits through CRISPR/cas genome editing in woody species to improve forest diversity and yield., Critical Reviews in Biotechnology, 43, 6, 884–903, https://doi.org/10.1080/07388551.2022.2092714, 9.6
  • Sujitha, D., Kumar, H.G.J., Thapliyal, G. et al., Transcription factors controlling the expression of oxidative stress associated genes in rice (Oryza sativa L.)., Plant Biotechnol Rep, 17, 835–851, 2023, https://doi.org/10.1007/s11816-023-00819-8, 2.0
  • Bhandari, M.S., Dabral, A., Maikhuri, S., Bisht, A., Thapliyal, G., Kant, R., Meena, R.K., Kumar, D. and Rana, V., Genetic evaluation and characterization of anatomical and physicochemical properties in Grevillea robusta: an alternative commercial agroforestry species., Journal of the Indian Academy of Wood Science, 20, 2, 123-137, 2023, 0.7

Conferences

  • Poster presentation, Anomalous flowering in Populus deltoides Bartr., International seminar on Forestry and Allied Specific topics for innovation and R&D initiatives [FAST-2019], Dolphin (PG) Institute of Biomedical and Natural Sciences, Dehradun
  • Oral presentation, Developing molecular breeding technologies for enhancing plant productivity in degraded lands.
  • Poster presentation, Genetic alteration of guard cell specific ion channel regulating genes to improve carbon gain in P. deltoides., National Symposium Remembering Gregor Johann Mendel on his Bicentennial Birth Year, 05/05/22 - 05/06/22, Department of Genetics & Plant Breeding, College of Agriculture, G.B. Pant University of Agriculture & Technology, Pantnagar.
  • Poster presentation, Genetic modulation of stomatal aperture regulating genes to enhance carbon gain and yield in P. deltoides., National Conference on Sustaining Himalayan Ecosystems, 09/09/23, Department of Biotechnology, Department of Microbiology and Department of Food Science and Technology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand.

Academic chronicle

  • SSC, I.C.S.E, 79%
  • HSC, I.S.C, 67%
  • B.Sc, Biotechnology, H.N.B Garhwal University, Srinagar, Uttarakhand, India, 58%
  • M.Sc, Biotechnology, Graphic Era University, Dehradun, Uttarakhand, India, 85%
  • PhD, Forest biotechnology, ICFRE-Forest Research Institute, Dehradun, Uttarakhand, India, Submitted

Areas Of Interest

  • Genetic improvement of forest species for enhanced resilience to environmental stressors.
  • Application of CRISPR-Cas9 and other gene editing technologies to improve productive traits and resilience in forest trees and crops.
  • Transcriptomics and functional genomics of plant stress responses to biotic and abiotic challenges
  • Tissue culture

Extracurricular Activities

First prize in science quiz in department of biotechnology Graphic Era University First prize in volleyball in interdepartmental volleyball tournament Third prize in badminton singles and doubles in interdepartmental badminton tournament Runner-up in basketball in interdepartmental basketball tournament

Timeline

Juniour Project Fellow

ICFRE-Forest Research Institute
01.2018 - Current

PhD. - Forest Biotechnology

ICFRE-Forest Research Institute

MSc. - Biotechnology

Graphic Era (Deemed to be University)

B.Sc - Biotechnology

H.N.B Garhwal University

SSC -

I.C.S.E

HSC -

I.S.C
Garima Thapliyal