Gayathri R

iPSC reprogramming and iN generation to model dementia

• Independently established and optimizing NGN2-mediated direct differentiation of human iPSCs into induced excitatory neurons.
• Contributed to the development and optimization of human iPSC-derived neuronal platforms to model neurodegenerative mechanisms.
• Perform lentiviral production and transduction, molecular assays, functional readouts, and quantitative data analysis.
• Collaborate closely with experimental and data-focused researchers; present findings in lab meetings and internal reviews.

Activity mediated mitochondrial protein synthesis

• Contributed to investigate activity-dependent protein synthesis following NMDA receptor stimulation, and also in the background of Alzheimer's Disease.

• Studied cellular and mitochondrial health in human iPSC-derived neural progenitor cells under ethanol-induced neurotoxic stress.
• Assessed ROS production, mitochondrial dysfunction, and cell viability using flow cytometry and biochemical assays.

• Investigated the impact of altered glucose conditions on neurite outgrowth in retinal ganglion cells.
• Studied the role of the cell adhesion molecule Caspr1 in neurodegenerative morphological changes.

• Gained exposure to industry-oriented biological research workflows and laboratory operations.