Gulshan Kumar

• Father's Name: Sh. Baljit Kumar
• Date of Birth: 09/24/96
• Place of Birth: Bathinda
• Gender: Male
• Nationality: Indian
• Marital Status: Unmarried
• Phone number: +91 8360655398, +91 9417027048

• English
• Hindi
• Punjabi

• 2021 – Qualified GATE 2021 Life Science
• 2020 – Selected for JNCASR-SRFP Summer research Fellowship
• 2019 – Qualified joint CSIR-UGC NET 2019

Cell Culture Techniques

• Basic Animal Cell Culture Techniques: Media preparation and filtration, thawing, trypsinization and splitting the mammalian cells, cell seeding, drug treatment, assessed protective and therapeutic potential of nanoparticles on cells, cryopreservation of cells
• MTT assay, Resazurin assay, Live Dead assay, DCFH-DA assay, Immunofluorescence.
• Developed diabetic retinopathy in vitro model: on Human Retinal Pigment Epithelium cells (ARPE-19)
• Developed plasticizer induced male infertility in vitro model: on TM3 Leydig cells
• Handled Human neuroblast cells (SH-SY5Y)

Molecular Biology Techniques

• DNA isolation, RNA extraction, cDNA synthesis, RT-PCR, SDS-PAGE and Western Blot

Animal Handling and Research

• Mice handling, Different routes of administration, Dissection, Perfusion, Tissue Harvesting, Tissue Processing, Tissue Sectioning and Staining, Microscopic Tissue Analysis (Certificate of "Hands on Training on Basics of Animal Handling (Mice) and Research" organized by IISER Kolkata, Animal Facility, from 1st to 15th November, 2023, Coordinated by Prof. Jayasri Das Sarma)

Nanoparticle Synthesis and Characterization

• Synthesis and characterization of plant-based metal nanoparticles i.e. Selenium and Zinc nanoparticles, drug loaded Polydopamine nanoparticles and drug loaded Human Serum Albumin nanoparticles respectively.
• Drug quantification by HPLC and determination of encapsulation efficiency
• Drug release kinetic study
• Assessment of biochemical activity of plant-based metal nanoparticles through various biochemical assays: DPPH free radical scavenging assay, Ferric reducing ability of plasma assay (FRAP), H2O2 scavenging activity assay, Superoxide dismutase (SOD) assay, Assessment of Hemo-compatibility - Hemolytic test for nanoparticles

Plant Extract Preparation 

• Plant extraction in ethanol and estimation of antioxidant potential of plant extract through various biochemical assays: DPPH free radical scavenging assay, Ferric Reducing Antioxidant Power Assay (FRAP), Total phenolic content (TPC), Total Flavonoid content (TFC).

Instruments Handled

• UV-Visible Spectrophotometer, Melvern Zetasizer, Fourier Transform Infrared (FTIR) Spectrophotometer, Cytation 5 from Biotek Synergy, Buchi Rotavapor, Bath and Probe Sonicator, Homogenizer, pH meter, Microscopes: Euromex Oxion Inverted microscope, Olympus Evos Inverted microscope, Euromex stereo microscope, Small Upright Compound Microscope

Sharma, P., Kishore, A., De, I., Negi, S., Kumar, G., Bhardwaj, S., & Singh, M. (2023). Mitigating neuroinflammation in Parkinson's disease: Exploring the role of proinflammatory cytokines and the potential of phytochemicals as natural therapeutics. Neurochemistry International, 105604.

• Certificate of participation in the E-Quiz on ‘Immunology’ jointly organized by the Department of Biotechnology and Department of Environmental Science of “Shree Ramkrishna Institute of Computer Education & Applied Sciences M.T.B. College Campus, Athwalines, Surat-395 001, and scored 90/100, on 23rd May 2020.
• Certificate of completion of Summer Academic & Internship Program on “Bioescalator: Insights into Biotechnology, Plant Science and Bioinformatics” organized by Department of Biotechnology, IILM College of Engineering & Technology, Greater Noida, held between 11th May - 5th June 2020.
• Certificate of participation in “SPARC-UNDO US Immunology workshop” jointly organized by India institute of technology Ropar, India and The George Washington University, USA, on June 12-13th 2020.
• Certificate of attending 6-days long INYAS-CUPB Lecture Workshop ‘tangled bank’ encompassing 15 lectures organized by Indian National Young Academy of Sciences and Department of Botany, Central University of Punjab, Bathinda, India in connection with World Environment Day, held between 31st May and 5th June, 2020.
• Certificate of Completion of Course on “Artificial Intelligence & Drug Discovery” organized by Shri Vithal Education & Research Institute’s College of Pharmacy, Pandharpur, Maharashtra, India, on 21stApril, 2020.
• Certificate of participation in the webinar on “Scribere Scientia: An Insight into Scientific writing” organized by M.Sc. students of plant biology and biotechnology (2019-2021) under the aegis of Department of Plant Sciences, School of life sciences, University of Hyderabad, Telangana, India, on 10th June 2020.
• Certificate of participation in online Pandemic COVID-19 Awareness programme organized by Office of District Coordinator N.S.S., Sri Ganganagar (Raj.) & committed to discharge the service to the Nation as a Responsible Citizen, on 11th May, 2020.
• Certificate of participation in the National webinar on “Opportunities of the microbiologists in the new normal era” organized by Department of microbiology Vidyasagar University Midnapore Werst Bengal India, on 26-27th September 2020.

• Seminar on “Designer quantum matter in van der Waals heterostructures” by Dr. Somesh C. Ganguli, (Aalto University, Finland), Institute of Nano Science and Technology, Mohali on 20th May, 2023.
• Seminar on “Adaptive down- and up-conversion "by Prof. Dirk Michael Guldi, Co-Editor-inchief: Nanoscale and Nanoscale Horizons, INST to Institute of Nano Science and Technology, Mohali on 31st March, 2023.
• Lecture on “Light as a Reagent and Product.” By Prof. Abraham Joy (Univ. of Akron, Ohio)’s talk will focus on “Macromolecular Strategies to Address Biofilms and Bacterial Colonization and Their Application to Wound Healing”, Institute of Nano Science and Technology, Mohali on March 18th, 2023.
• Talk on "Affordable clean water using advanced materials" by Prof. T. Pradeep, BoG Chairman, Institute of Nano Science and Technology, Mohali on 15th March 2023.
• Seminar on “RSC meet the editor event at INST” organized by Institute of Nano Science and Technology, Mohali. Speakers: Dr. Richard Kelly (Executive Editor, Chem Comm, Chem SocRev), Professor Sandeep Verma (Associate editor, Chem Comm), and Dr. Aprana Ganguly (EDM, RSC) on 7th Feb 2023
• Talk on "Single-walled carbon nanotube growth by alcohol catalytic chemical vapor deposition" by Prof. Takahiro Maruyama (Meijo University, Nagoya Japan), Institute of Nano Science and Technology, Mohali, on 22nd Feb 2023
• CSIO-INST Bilateral Meet on 28th June 2023, INST-IISER bilateral meeting on 14-15 March 2022, Bilateral Meeting of INST- CSIR-IMTECH Chandigarh on 29th July 2022.

• "Hands on Training on Basics of Animal Handling (Mice) & Research" in Animal Facility of IISER KOLKATA organized by prof. Jayasri Das Sarma, from 1st to 15th November 2023.
• Waters Workshop on Advance LC and MS Technologies organized by Waters India Pvt. Ltd - Chandigarh on July 31st, 2024.

Dr. Sunil Kumar Singh

Associate Professor, Department of Zoology, School of Basic and Applied Sciences, Central University of Punjab, Bathinda, Punjab, India.

Email id: sunil.singh@cup.edu.in

Phone no. +919450950549, +917973206169

Dr. Abhinoy Kishore

Assistant Professor, Chandigarh Group of Colleges, Landran, Punjab, India.

Email id: abhinoy@gmail.com

Phone no. +917250610999

Prof. Paramdeep Kaur, Retired Associate professor (HOD), Department of Zoology, Rajindra College Bathinda, Punjab, India.

Email id: paramdeep1961@gmail.com

Phone no. +91941731104