Hridya Chandrasekhar

Molecular Biology:

• Experience in basic molecular biology techniques including nucleic acid isolation, molecular cloning, site directed mutagenesis, electrophoresis, western blot, quantitative RT PCR based expression analysis, immunoprecipitation and affinity tagged protein purification.
• Experience in conducting and screening of large protein-protein interaction sets using yeast two hybrid assays.

Cell Biology:

• Culturing and experimentation on bone marrow derived macrophages (BMDMs) and various cell lines (HCT-8, HeLa, RAW 264.7, CaCo2, HEK-293T, MCF-7, J774, NIH-3T3).
• Silencing and over expression of genes using transfection reagents, lentiviral transduction, or nucleofection.

Mice handling and enteric infection study models:

• Well versed in studying enteric infections in mice using oral gavage-based methodologies.
• Dissection and harvesting of organs such as colon, small intestine, MLNs, spleen, liver, and gall bladder.
• Expertise in evaluating and enumerating pathogen load and pathology during enteric infections.
• Colon sectioning, Hematoxylin and Eosin staining for histopathology analysis.

Microbiology:

• Experience in handling microbial cultures including pathogens like E. coli, Salmonella Typhimurium and Saccharomyces cerevisae.
• Well versed in bacterial transformations, site directed mutagenesis and protein expression.