Hi, I’m
JAGANATH ARUNACHALAM
WORAIYUR, TRICHY,India
Summary
Researcher with five years of Junior research fellowship in Basic and Clinical Immunology (inflammation and innate immunity) with strong demonstration skills in in-vitro studies (primary and transformed cells), Molecular Biology, Proteomics and Immunophenotyping. Strong research professional who earned a master's degree in Bio-Medical Sciences. Proven abilities to evaluate data critically, quickly become proficient in new subjects and techniques, identify key concepts, and troubleshoot problems. Work productively independently and in teams, multi-task, set and meet deadlines, and supervise others.
Overview
8
years of professional experience
5
years of post-secondary education
Work history
MCF, Dharmashila Narayana Superspeciality HospitalNew Delhi, India
Junior Research Fellow
12.2020 - 04.2023
Job overview
- Plan and manage research projects studying the Mitigating COVID-19 infection & progression via innate immune modulation and NK cell and Monocyte biology in Post Haplo identical Transplant patients
- Demonstrated various molecular biology and immunological skill sets: Immunophenotyping (Flow Cytometry) q-RT-PCR, ELISA, Immune Cell isolation such as NK, Monocyte and T-cells from Blood, PBMC and Bone Marrow
- Attend weekly review meetings with other basic laboratory experiments, data collection, and analysis
- Writing research manuscripts, giving and receiving critiques on works in progress
- Regularly present research data and pertinent background information gathered from published literature at weekly lab meetings
- Participate in weekly journal club discussions to critique and analyze relevant scientific journal articles
- Created and maintained goals and progress reports for multiple research projects in addition to a detailed daily laboratory notebook with data.
- Coded data following specified research protocol and entered data into a computer database for subsequent analysis.
- Prepared graphs and spreadsheets to present findings.
Birla Institute Technology and SciencesHyderabad, India
Research Assistant
02.2019 - 10.2020
Job overview
- Plan and manage research project studying “To understand the role of gut-mircobiota derived bile acids in regulating macrophage driven intestinal inflammation”.
- Studied the effect of bile acids on macrophage polarization and studied immunoregulatory macrophage.
- Studied TLR, NLR, RLR, and inflammasome pathways.
- Demonstrated various molecular biology skill sets: q-RT-PCR, Western-Immuno Blotting, ICC, IF, ELISA, HPLC, RNAi, in-vitro studies, Colorimetric, and fluorescent assays.
- Set up, calibrated and maintained field or laboratory equipment as per study requirements.
- Followed up with research study participants to acquire additional data or resolve data inaccuracies.
- Prepared graphs and spreadsheets to present findings.
- Checked facts and proofread and edited research documents for accuracy.
- Set up and maintained research database to avoid loss of information.
Chettinad Academy of Research and EducationChennai, India
Project Assistant
07.2018 - 02.2019
Job overview
- Studied the “Role of human umbilical cord mesenchymal stem cells (UC-MSCs) and UC-MSC derived conditioned medium in regulation of colon cancer progression: An approach towards elucidating function of paracrine factors and epigenetic regulators”.
- Created and maintained goals and progress reports for multiple research projects in addition to a detailed daily laboratory notebook with data.
- Trained and supervised various undergrad/masters' student's dissertation work.
- Gathered data to create Excel spreadsheets for budget and inventory management.
- Created databases and spreadsheets to improve inventory management and reporting accuracy.
Dr Ambedkar Center for Biomedical Sciences, University of DelhiNew Delhi, India
Summer Research Intern
05.2016 - 06.2016
Job overview
- Worked on the project ‘Taurine partially inhibits the chaperone activity of α-casein and β-casein
- As a summer fellow, I acquired practical knowledge about Biophysical methods such as UV-vis spectrophotometer, CD spectroscopy and Fluorescence spectroscopy.
- Set up, calibrated and maintained field or laboratory equipment as per study requirements.
Department of Biomedical Sciences, Bharathidasan UTiruchi, India
Summer Research Intern
05.2015 - 07.2015
Job overview
- Studied Doxorubicin-induced female reproductive toxicity: an assessment of ovarian follicular apoptosis, cyclicity and reproductive tissue histology in Wistar rats.
- As a trainee, I got hands-on experience on animal handling and maintenance, determination of different stages of estrous cycle, cytogenic assay, histopathology studies, and immunohistochemistry. Has the ability to perform the abovementioned techniques independently.
- Research work was published in Drug and chemical toxicology- scientific journal.
- Wrote reports to summarise data and implications of results.
Education
Bharathidasan UniversityTiruchi, Tamil Nadu
M.Sc. from Bio-Medical Sciences
07.2013 - 04.2018
University overview
- I have excelled in courses like Immunology, Molecular Biology, Biochemistry and Microbiology.
- Received Summer Research Fellowship Award in 2018
- During my master's, I had an opportunity to carry out my master's thesis/dissertation at India's preliminary University (Delhi University).
- I worked on the project to understand the effect of T177i mutant of peroxiredoxin 6: A potential protective variant for neurodegenerative diseases.
- Demonstrates skills in Protein purification, Molecular cloning, plasmid isolation, Western blotting and ELISA.
Skills
- Animal Studies: Well-trained in animal colony management (Breeding, weaning, experimental protocols), Small animal handling (rat and mice) and maintenance, Mice and Rat Necropsy, Organ collection, and Histology
- Gene Expression studies - Gradient PCR, RT PCR, Primer design, Quantification, Genotyping
- Protein Expression systems - Western Blotting, ELISA, Immunophenotyping and Immunoblot
- Fluorometric assays, Immuno-Histochemistry, Immuno-Fluorescence
- In-vitro Cell culture / Imaging Techniques: Expert in maintenance and working with various human and mouse cell lines and primary cells such as NK, Mono and T-cells Drug induction studies, cell proliferation, apoptosis assays, MMP, apoptotic morphological changes in a fluorescent microscope using different probe staining, and Confocal microscopy
- Biophysics Skills: Hands-on experience in CD spectroscopy, fluorescence spectroscopy and UV-vis spectroscopy for the protein structural and functional studies
- Analytical Skills: Hands-on experience in HPLC system for the detection of polyamines
- RNA Seq Skills: Hands-on experience in bulk RNA seq using long-read sequencing by Oxford nanopore technologies In RNA seq have expertise in RNA isolation and purification, poly-A selection, and library preparation for direct cDNA and PCR-cDNA methods
- Computer Skills: I have well knowledge I handling statistical software such as GraphPad Prism and IBM SPSS software Expert in making scientific cartoons and pictures using MS PowerPoint
- Scientific Writing: I exhibit commendable scientific writing and communication skills in English Grant Writing / Proposal Development, concept development, Budget Preparations, Cost sharing evaluations
Publications
- Jaiswal, S.R.; Saifullah, A.; Arunachalam, J. et al., Augmenting Vaccine Efficacy against Delta Variant with ‘Mycobacterium-w'-Mediated Modulation of NK-ADCC and TLR-MYD88 Pathways. Vaccines 2023, 11, 328.
- Jaiswal, S.R., Arunachalam, J., et al., 2022. Impact of an Immune Modulator Mycobacterium-w on Adaptive Natural Killer Cells and Protection Against COVID-19. Frontiers in immunology, 13, p.887230.
- Jaiswal, S.R., Arunachalam, J., et al., 2022. Impact of adaptive natural killer cells, KLRC2 genotype and cytomegalovirus reactivation on late mortality in patients with severe COVID‐19 lung disease. Clinical & translational immunology, 11(1), p.e1359.
- Nishi, K., Gunasekaran, V.P., Arunachalam, J. and Ganeshan, M., 2018. Doxorubicin-induced female reproductive toxicity: an assessment of ovarian follicular apoptosis, cyclicity and reproductive tissue histology in Wistar rats. Drug and chemical toxicology, 41(1), pp.72-81.
Book Chapter:
- Marotta, F., Arunachalam, J ., et al., 2019. Oxidative Stress and Smoke-Related Lung Diseases: A Tentative Approach Through the Blood, Lungs, and Gut. In Oxidative Stress in Lung Diseases (pp. 27-50). Springer, Singapore.
Academic References
- Dr Rimpy Kaur Chowhan PhD
Assistant Professor
Aacharya Narendra Dev College
University of Delhi, Delhi, INDIA
Email ID: rimpykaurchowhan@andc.du.ac.in - Dr Vivek Sharma PhD
Assistant Professor
Department of Biological Sciences
B-209, BITS Pilani-Hyderabad Campus
Jawahar Nagar, Kapra Mandal, Medchal district
Hyderabad- 500 078, Telangana, INDIA.
Email ID: viveksharma@hyderabad.bits-pilani.ac.in
Timeline
Junior Research Fellow
MCF, Dharmashila Narayana Superspeciality Hospital
12.2020 - 04.2023
Research Assistant
Birla Institute Technology and Sciences
02.2019 - 10.2020
Project Assistant
Chettinad Academy of Research and Education
07.2018 - 02.2019
Summer Research Intern
Dr Ambedkar Center for Biomedical Sciences, University of Delhi
05.2016 - 06.2016
Summer Research Intern
Department of Biomedical Sciences, Bharathidasan U
05.2015 - 07.2015
Bharathidasan University
M.Sc. from Bio-Medical Sciences
07.2013 - 04.2018
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