Jayachandra Reddy Nakkala

Dedicated and accomplished researcher with a Ph.D. In Biochemistry and Molecular Biology from Pondicherry University, India, along with extensive postdoctoral experience in Cancer, Regenerative Medicine, and Vaccines. Committed to advancing scientific knowledge, I am eager to contribute my expertise in a challenging and innovative environment. Known for adaptability to the fast-paced research landscape, I excel both independently and collaboratively.

Molecular & Cellular Biology
Cell culture,Cloning, RT-PCR, RNA/DNA isolation, protein expression (E. coli, insect cells), protein labeling (fluorescent dyes), single-cell isolation (lymph nodes, spleen, lungs, skin, muscles), bone marrow-derived macrophage/dendritic cell differentiation, peritoneal macrophage isolation, proliferation assays (³H-thymidine, CFSE), MTT assay, trypan blue quenching.

Protein Biochemistry & Nanotechnology
Protein purification (size exclusion, affinity, ion exchange, Ni-NTA), Western blot, ELISA, immunoprecipitation, IP/pull-down for mass spectrometry, HAI titers, plaque assay. Nanoparticle preparation & characterization (metal, nanofibers, lipid NPs, virus-like particles), SEM/TEM, DLS, FT-IR, ICP-MS, ultracentrifugation, vesicle/membrane isolation.

Immunology & Flow Cytometry
Multicolor flow cytometry (Cytek Aurora, up to 28-color panels; BD FACS), macrophage polarization, T-cell activation, histology, immunofluorescence. Data analysis: FlowJo, FCS Express, GraphPad Prism, Origin, ImageJ.

Animal Models & In Vivo Techniques
Murine tumor models: 4T1/4T1-Luc, LLC/LLC-Luc, MC38, B16F10. Inoculation routes (i.v., i.n., i.d., s.c., i.m., oral). Mammary fat pad inoculation. Irradiation (SmART+). AMI/IVIS imaging, IncuCyte live imaging. Mouse phenotyping, genotyping, colony maintenance (weaning, breeding, inventory).

Virology
Work with influenza strains (PR8, pdm09, H3N2). Virus adaptation and collection, neutralization assays, HAI, plaque assays, virus challenge.

Transgenic Mouse Models
Extensive experience with KO/KI strains: TLR2 KO, TLR4 KO, TLR2/4 DKO, MyD88 KO, HSP70 KO, CD137 KO, hCD137 KI, OT-1, OT-2.

· Qualified for CSIR-Junior Research Fellowship - National Eligible Test with All India Rank – 38 (CSIR-JRF-NET) (CSIR-June 2013). A national open competitive entrance exam organized by Council for Scientific and Industrial Research (CSIR) and University Grant Commission (UGC) for graduate fellowship to pursue graduate (Ph. D) studies in India

· Qualified Graduate Aptitude Test in Engineering (GATE) in 2009, 2010, 2011, and 2012A national open competitive exam conducted by all the Indian Institute of Technologies (IITs)

· Qualified for Lectureship in the CSIR-JRF-NET exam (CSIR-June 2012)

· Qualified Andhra Pradesh State Eligibility Test (APSET) in 2012

· Excellent article award from Journal of Material Science and Technology (Evaluated from 2016 to 2019 publications Elsevier)

· Qualified SERB for postdoctoral research in IITH, India 2017

• Potent adjuvantation of influenza vaccination and induction of type I interferon-stimulated proteins by CpG 1018/cGAMP combination adjuvant, Yibo Li, Jayachandra Reddy Nakkala, Labone Akter, Xinyuan Chen, European Journal of Immunology, Accepted
• Dual roles of in situ generated HSP70 in antigen delivery and immunoregulation, Xinliang Kang, Zhuofan Li, Jayachandra Reddy Nakkala, Yibo Li, Labone Akter, Yiwen Zhao, Xinyuan Chen, Frontiers in Immunology, Accepted
• Augmentation of mRNA vaccine-induced cellular immune responses and anti-tumor immunity by CpG 1018 adjuvant, Yibo Li, Jayachandra Reddy Nakkala, Labone Akter, Xinliang Kang, Yuna Song, Elise VanLuinen, Xinyuan Chen, Cancer Immunology Research, Under review
• Flagellin co-expression potentiates mRNA vaccine-induced cytotoxic T lymphocyte responses but not anti-tumor immunity, Yibo Li, Jayachandra Reddy Nakkala, Labone Akter, Xinyuan Chen, Scientific Reports, Accepted
• Differential regulation of DC function, adaptive immunity, and MyD88 dependence by MF59 and AS03-like adjuvants, Jayachandra Reddy Nakkala, Yibo Li, Labone Akter, Xinliang Kang, Xinyuan Chen, Vaccines, 2024, 12, 531
• Therapeutic role of biogenic silver and gold nanoparticles against a DMH-induced colon cancer rat model, Rani Mata, Jayachandra Reddy Nakkala, Sudha Rani Sadras, Biomaterials Advances, 2023, 146, 213279
• Immuno-modulating theranostic gold nanocages for the treatment of rheumatoid arthritis in vivo, Rajiu Venkatesan, Hao Xiong, Yuejun Yao, Jayachandra Reddy Nakkala, Tong Zhou, Shifen Li, Cunyi Fan, Changyou Gao, Chemical Engineering Journal, 2022, 446, 136868
• Macrophage membranes-functionalized nanofibrous mats and their immunomodulatory effects on macrophage polarization, Jayachandra Reddy Nakkala, Yiyuan Duan, Jie Ding, Wali Muhammad, Deteng Zhang, Zhengwei Mao, Hongwei Ouyang, Changyou Gao, Acta Biomaterialia, 2022, 141, 24-38
• Dimethyl Itaconate-Loaded Nanofibers Rewrite Macrophage Polarization, Reduce Inflammation, and Enhance Repair of Myocardic Infarction, Jayachandra Reddy Nakkala, Yuejun Yao, Zihe Zhai, Yiyuan Duan, Deteng Zhang, Zhengwei Mao, Linrong Lu, Changyou Gao, Small, 2021, 2006992
• Immunomodulatory Biomaterials and Their Applications in Therapies for Chronic Inflammation- Related Diseases, Jayachandra Reddy Nakkala, Ziming Li, Wajiha Ahmad, Kai Wang, Changyou Gao, Acta Biomaterialia, Review article
• A Reactive Oxygen Species Savenging and O2 Generating Injectable Hydrogels for Myocardial Infraction Treatment In vivo, Jie Ding, Yuejun Yao, Jiawei Li, Yiyuan Duan, Jayachandra Reddy Nakkala, Xue Feng, Wangbei Cao, Yingchao Wang, Liangjie Hong, Liyin Shen, Zhengwei Mao, Yang Zhu, Changyou Gao, Small, 2020, 16, 2005038
• Unsaturated polyurethane films grafted with enantiomeric polylysine promotes macrophage polarization to a M2 phenotype through PI3K/Akt1/mTOR axis, Tiguan Dean, Honcho Zhang, Zehua Li, Yuejun Yao, Jie Ding, Xuemei Wang, Jayachandra Reddy Nakkala, Deteng Zhang, Zhaoyi Wang, Xingang Zuo, Xiaowen Zhang, Jun Ling, Changyou Gao, Biomaterials, 2020, 246, 120012
• Micro-and nanoparticles-based immunoregulation of macrophages for tissue repair and regeneration, J Ding, R Venkatesan, Z Zhai, W Muhammad, Jayachandra Reddy Nakkala, Changyou Gao, Colloids and Surfaces B: Biointerfaces, 2020, 192, 111075, Review article
• Surface-Anchored Graphene Oxide Nanosheets on Cell-Scale Micropatterned Poly (d,l-lactide-co-caprolactone) conducts Promote Nerve Regeneration, Deteng Zhang, Yuejun Yao, Yiyuan Duan, Xing Yu, Haifei Shi, Jayachandra Reddy Nakkala, Xingang Zuo, Liangjie Hong, Zhengwei Mao, Changyou Gao, ACS applied materials & interfaces, 2020, 12, 7915-7930
• Green synthesized silver nanoparticles: Catalytic dye degradation, in vitro anticancer activity and in vivo toxicity in rats, Jayachandra Reddy Nakkala, Rani Mata, Kumar Raja, VK Chandra, Sudha Rani Sadras, Materials Science and Engineering: C, 2018, 91, 372-381
• In vivo bio-distribution, clearance and toxicity assessment of biogenic silver and gold nanoparticles synthesized from Abutilon indicum in Wistar rats, Rani Mata, Jayachandra Reddy Nakkala, VK Chandra, Kumar Raja, Sudha Rani Sadras, Journal of Trace Elements in Medicine and Biology, 2018, 48, 157-165
• Green synthesized nano silver: Synthesis, physicochemical profiling, antibacterial, anticancer activities and biological in vivo toxicity, Jayachandra Reddy Nakkala, Rani Mata, Sudha Rani Sadras, Journal of Colloid and Interface Science, 2017, 499, 33-45
• The antioxidant and catalytic activities of green synthesized gold nanoparticles from Piper longum fruit extract, Jayachandra Reddy Nakkala, Rani Mata, Sudha Rani Sadras, Process Safety and Environmental Protection, 2016, 100, 288–294
• Polyphenol stabilized colloidal gold nanoparticles from Abutilonindicum leaf extract induce apoptosis in HT-29 colon cancer cells, Rani Mata, Jayachandra Reddy Nakkala, Sudha Rani Sadras, Colloids and Surfaces B: Biointerfaces, 2016, 143, 499–510
• Green synthesis of silver and gold nanoparticles from Gymnema sylvestre leaf extract: study of antioxidant and anticancer activities, Jayachandra Reddy Nakkala, Rani Mata, Ekta Bhagat, Sudha Rani Sadras, Journal of Nanoparticle Research, 2015, 17, 151
• Comparative Study of Antioxidant and Catalytic Activity of Silver and Gold Nanoparticles Synthesized from CostuspictusLeaf Extract, Jayachandra Reddy Nakkala, Ekta Bhagat, Kitlangki Suchiang, Sudha Rani Sadras, Journal of Materials Science & Technology, 2015, 31, 986-994
• Catalytic and biological activities of green silver nanoparticles synthesized from Plumeria alba (frangipani) flower extract, Rani Mata, Jayachandra Reddy Nakkala, Sudha Rani Sadras, Materials Science and Engineering C, 2015, 51, 216–225
• Biogenic silver nanoparticles from Abutilon indicum: Their antioxidant, antibacterial and cytotoxic effects in vitro, Rani Mata, Jayachandra Reddy Nakkala, Sudha Rani Sadras, Colloids and Surfaces B: Biointerfaces, 2015, 128, 276–286
• Fenugreek seed extract and its phytocompounds - trigonelline and diosgenin arbitrate their hepatoprotective effects through attenuation of endoplasmic reticulum stress and oxidative stress in Type 2 Diabetic rats, M. Tharaheswari, Jayachandra Reddy Nakkala, J. Syam Praveen Kumar, R. Kumar, K.C Varshney, M. Vasanth Kumar, S. Sudha Rani, European Food Research and Technology, 2015, 240, 223-232
• Biological activities of green silver nanoparticles synthesized with Acorouscalamus rhizome extract, Jayachandra Reddy Nakkala, Rani Mata, Gupta AK, Sudha Rani Sadras, European Journal of Medicinal Chemistry, 2014, 85, 784-794
• Evaluation of antioxidant, antibacterial and cytotoxic effects of green synthesized silver nanoparticles by Piper longum fruit, Jayachandra Reddy Nakkala, D. Nagoor Vali, M. Rani, S. Sudha Rani, Materials Science and Engineering C, 2014, 34, 115–122
• Trigonelline and diosgenin attenuate ER stress, oxidative stress mediated damage in pancreas and enhances adipose tissue PPARγ activity in type 2 diabetic rats, M. Tharaheswari, J. Syam Praveen Kumar, Jayachandra Reddy Nakkala, S. Subhashree, S. Sudha Rani, Molecular and Cellular Biochemistry, 2014, 396, 161-174
• Fenugreek seed extract stabilize plasma lipid levels in type 2 diabetes by modulating PPARs and GLUT4 in insulin target tissues, M. Tharaheswari, J. Syam Praveen Kumar, Jayachandra Reddy Nakkala, S. Subhashree, S. Sudha Rani, AJPCT, 2014, 2, 587-602

• Bilateral Conference on Polymer Science and Engineering, 2019-01-01, Zhejiang University, China, Poster presentation
• 1st International Conference on Nanoscience and Nanotechnology (ICNAN ’16), 2016-10-19, Centre for Nanotechnology Research VIT University, Vellore, Tamilnadu, India, Oral presentation
• National conference on Drug Discovery and Cancer Therapy (DDCT-2016), 2016-02-25, Dept. of Biochemistry and Molecular Biology, Pondicherry University, Pondicherry, India, Oral presentation
• Fourth International Conference on Nanomedicine and Tissue Engineering (ICNT-2016), 2016-08-12, Mahatma Gandhi University, Kottayam, Kerala, India, Poster presentation
• Innovations and Future Research Dimensions on Nano Biopharmaceutical Technology, 2014-10-07, Dept of Pharmaceutical technology, Anna University, Tamil Nadu, India, Oral presentation
• 4th International Conference on Stem Cells and Cancer: Proliferation Differentiation and Apoptosis (ICSCC-2013), 2013-10-19, Haffkine University, Mumbai, India, Poster presentation
• Techniques in Pre-Clinical studies, 2015-12-17, Dept. of Biotechnology, Pondicherry University, Pondicherry, India, Participation
• Emerging Trends in Biochemistry and Molecular Biology, 2014-03-17, Dept. of Biochemistry and Molecular Biology, Pondicherry University, Pondicherry, India, Organizing committee member
• Drug Discovery & Cellular Dynamics, 2013-03-21, Dept. of Biochemistry and Molecular Biology, Pondicherry University, Pondicherry, India, Participation
• Current Scenario and Emerging Trends in Hepatocellular Diseases (NC-HepCD-2012), 2012-10-05, Dept. of Biochemistry and Molecular Biology, Pondicherry University, Pondicherry, India, Participation

• Prof. S. Sudha Rani, Ph.D Guide, Dept. of Biochemistry and Molecular Biology, Pondicherry University, Puducherry, 605 014, India, sadrassudha@yahoo.com, +91-413-2654404
• Prof. K. Srikumar, Dept. of Biochemistry and Molecular Biology, Pondicherry University, Puducherry, 605 014, India, srikumarprof@gmail.com, +91-413-265-4422
• Prof. Changyou Gao, Ph.D (Postdoctoral Guide), Dept. of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China, +86-571-87951108, 13858018768, cygao@zju.edu.cn
• Dr. Xinyuan Chen, Ph.D (Postdoctoral Guide), Dept. of Biomedical and Pharmaceutical Science, University of Rhode Island, Kingston, 02881, USA, +1-401-874-5033, xchen14@uri.edu
• Prof. Haval Shirwan, Ph.D (Postdoctoral Guide), Dept. of Pediatrics, University of Missouri-Columbia, Missouri, USA, +1-573-884-6720, haval.shirwan@health.missouri.edu