KESHARTHINI KARTHIKEYAN

Project - "Extracellular Tau oligomer internalization by microglial cells"

• Prepared and validated different species of Tau protein (monomers, oligomers, and aggregates) for cellular internalization experiments
• Executed microglial cell culture procedures, including cell revival, passaging, counting, viability assessment, and seeding to support experimental research.
• Performed immunofluorescence, SDS-PAGE, Western blotting, and ThS/ANS assays.
• Used fluorescence microscopy and cellular markers to investigate Tau uptake and localization.
• Cultivated skills in experimental troubleshooting, research planning, scientific literature review, and laboratory documentation to enhance research quality.
• Worked under guidance of Dr. Subashchandrabose Chinnathambi, Additional professor, Department of Neurochemistry.

Project- " Mobile mosquitoes - Understanding the entangled mobilities of human and Aedes mosquitoes in Mexico, Tanzania, India and Germany."

• Conducted fieldwork to collect Aedes mosquitoes near public transport systems, contributing to understanding mosquito distribution patterns.
• Used mosquito surveillance methods and equipment, including BG-Sentinel traps, oviposition traps, larval dipping, and Prokopack aspirators.
• Processed and classified mosquito samples, and collected field data through questionnaires to support ongoing research efforts.
• Performed DNA extraction, PCR, and agarose gel electrophoresis.
• Supported blood-meal analysis and preparation of samples for DNA sequencing.
• Analyzed genetic data using peak analysis, MEGA, and STRUCTURE software to identify genetic variations.

• Applied ICD-10-CM and HCC coding concepts to medical documentation.
• Acquired in-depth knowledge of medical terminology, anatomy and physiology, diagnoses, symptoms, and medication terminology to enhance coding accuracy.
• Facilitated accurate medical record interpretation and coding documentation to ensure compliance with coding standards.

Project-“Cloning, expression of full-length nCOVID-19 spike protein (S1) in E.coli expression vector.”

• Gained practical exposure to recombinant DNA preparation and protein expression.
• Worked with SDS-PAGE for protein analysis.

• Completed introductory training in ELISA and polyclonal antibody production to support laboratory research.