KRISHNA C R
Senior Scientist III
Summary
- Results-driven Next Generation Sequencing Specialist with extensive experience in end-to-end workflows.
- Proficient in operating advanced sequencing platforms including Illumina MiSeq, NextSeq 2000, and NovaSeq Xplus.
- Ensuring high-quality results and adherence to accurate standards.
- Core competencies include data analysis, quality control, and protocol optimization.
- Molecular Biology with extensive experience in diverse techniques, including PCR, cloning, and sequencing.
- Validating new NGS panels
- Competent in supplier relations and contract negotiations, ensuring timely delivery
- Proficient in NGS technologies, data analysis, and interpretation for HLA Typing
- Skilled in managing research projects from conception to completion, ensuring timely delivery of results.
Overview
21
21
years of professional experience
Work History
Senior Scientist III
Triesta Sciences
04.2016 - Current
Triesta Sciences (Unit of HCG Hospitals)
Senior Scientist III
- Performing sequencing of Miseq, Nextseq and Novaseq Xplus
- Validating new products through laboratory experiments
- Performing routine Clinical diagnostics test by
- Trusight Oncology 500 gene panel
- Trusight Hereditary cancer panel
- Ampliseq Myeloid panel
- AmpX V2 HLA typing
- Twist whole exome 2.0
- Nucleic acid extraction from FFPE block, Fresh tissue and blood samples
- Quality analysis of nucleic acid by real time PCR and Tape station
- HLA data analysis and reporting by NGS Engine
- Analysis, Interpretation, and authentication of reports
- Timely updates to clinicians regarding test results.
- Inventory & cost Management
- Establishing a new vendor for routine lab supplies and equipment
- Ensuring timely communication with FAS to seamless installation and troubleshoot experiments effectively.
Senior Scientific Officer
Merck Millipore
10.2007 - 08.2015
- Custom research projects
- Cloning and Protein purification
- DNA fingerprinting (RAPD, SCAR Marker Development, RFLP)
- Metagenomic Library
- Molecular based identification of microbes
- Product Improvisation
- Technical support and Customer orientation for products
Senior Research Fellow
Avesthagen Pvt Ltd
04.2005 - 10.2007
- Isolation and Characterization of Plant (Rice) Salt stress promoter and its validation
- Identification of SNPs responsible for virulence in Mycobacterium tuberculosis
- Cloning and expression of Bio therapeutically important protein and Technology transfer
Research Associate
Bhat Biotech India Pvt Ltd
03.2004 - 04.2005
- Recombinant protein HIV1-GP 41 purification
- Development of educational experimental teaching kits
Education
Master of Science - Biotechnology
Kuvempu University
Timeline
Senior Scientist III
Triesta Sciences
04.2016 - Current
Senior Scientific Officer
Merck Millipore
10.2007 - 08.2015
Senior Research Fellow
Avesthagen Pvt Ltd
04.2005 - 10.2007
Research Associate
Bhat Biotech India Pvt Ltd
03.2004 - 04.2005
Master of Science - Biotechnology
Kuvempu University