KRISHNA SAMPAT MULE

• Technical/Professional Skills:
• Agarose gel electrophoresis
• Isolation of bacterial and Bacterial characterization Preparation of WCB and MCB For new Probiotics strains
• Works on difference culture such as ( B.clausii, B.coagulans, B.Subtilis, L.Rhamnosus, L.reuteri, S.boulardii)
• Antimicrobial assay
• Good Laboratory Practice (GLP)
• cGMP
• Operation of Fermentor (20 lit, 30lit, 80 lit,1kl)
• Fermentation, (CIP, SIP, ESIP, FSIP,PHT)
• Batch monitoring - shake flask level, seed level, fed batch.
• TFF
• Analytical Techniques (TVC, MLT)
• Downstream Process ( Centrifugation, Spary drying, Lyophlization,Vaccum tray drying).
• Autoclave (B.I. test)

• Collaborated with leadership team to identify relevant questions and determine best methods of collection.
• Gathered, arranged and corrected research data to create representative graphs and charts highlighting results for presentations.
• Produced actionable information that improved {Yield}.
• Collaborated with staff to develop and implement best practices and accomplished all team goals.
• Collaborated with team members to initiate best practices to achieve organizational goals.

• Tracked collected specimens by initialing, dating and noting times of collection.
• Maintained strict aseptic fields when collecting biological samples, minimizing staff and patient infection risks.
• Recorded, reported and posted test results using [Software].
• Set up, maintained and verified sterility of lab equipment and tools.
• performed skill test like

- semen analysis

- IUI

- DFI

-PRP.