LAKKSHMI PRABHA V

Enthusiastic Junior embryologist eager to contribute to team success through hard work, attention to detail and excellent organizational skills. Clear understanding of WHO protocol and ESHRE recommendations Motivated to learn, grow and excel in the field of embryology. Compassionate Junior Embryologist with expertise in oocyte screening, sperm analysis and preparation, sperm cryopreservation and documentation. Methodical and detailed professional with an hands-on experience of 6 months. Comfortable working in a highly loaded stressful, fast-paced environment.

MSc Human Clinical Embryology and Assisted Conception:

Title: An analysis of variability between human acrosome reaction assessed by PSA, PNA & CD46

Duration: April 2021-July 2021

Brief Description:

One of the main aims in performing this study was to understand the efficiency of staining using multiple probes and to obtain results showing increased accuracy in detecting AR. The results of this study indicated that there is a significant difference observed between the probes used for assessing the AR showcasing that distinct staining profile of the AR dyes will provide differential results of AR. The results also showcase that staining using multiple probes will help in obtaining more efficient and accurate results regarding acrosome status. Staining using probes that bind to OAM cannot produce results as accurate as staining using multiple probes as once the spermatozoa has undergone acrosome reaction and outer membrane is shed, there is no way for binding to happen. Similarly, staining using IAM probes cannot effectively produce accurate results because if the spermatozoa has not undergone acrosome reaction, exposure of inner acrosomal contents does not occur. Thus, for future studies related to assessing AR, usage of more than one probe could be considered in order to obtain accurate results. This will further assist in understanding the sperm physiology and function

BTech Biotechnology:

Title: Influence of DDIT4 modulate the stem cell by activation of mTOR pathway in Breast Cancer

Duration: October 2018- April 2019

Brief Description:

In this study, we have analyzed various microarray datasets based on their experimental setup and the dataset GSE27515 has been narrowed down for which the enrichment and the pathway analysis have been performed. The differential gene expression studies have been performed to understand the expression and function of the genes involved in mTOR pathway where after a thorough analysis; a single gene DDIT4 has been narrowed down for further study. The Gene-Ontology studies for the functions of the genes have been analyzed simultaneously. The mRNA nucleotide sequence for the gene DDIT4 has been retrieved for which the primer has been designed and checked for their physical parameters. Simultaneously the RNA has been isolated from the cell lines and the cDNA was synthesized. The DDIT4 gene has been amplified using the cDNA. Further the plasmid DNA has been isolated from the pcDNA 3.1 (+) vector which was used for the process of cloning DDIT4 and has been purified using the PCR purification kit.

 Kapil Sanjay Sharma, Lakkshmi Prabha Venkatasubramani, Kavya Prasad, Bhamare Amruta Nitin, Ayyavu Mahesh. MarinOmics- Current and Future Perspectives. Nature Springer (Invited Chapter)

• Andrology- Sperm Analysis - Concentration, Motility, Morphology & Viability
• Sperm Preparation, Cryopreservation & Thawing
• Embryology – ICSI, Fert checks & Embryo grading
• Embryo Biopsy
• Sperm Acrosome assessment using flow cytometry
• Sperm DNA fragmentation
• Genomics- Microarray dataset analysis, Network analyst, PANTHER
• Molecular Biology- Plasmid DNA isolation, cloning of plasmid DNA; Western Blotting
• Primer designing
• Cell culture techniques

• Andrology- Sperm Analysis - Concentration, Motility, Morphology & Viability
• Sperm Preparation, Cryopreservation & Thawing
• Embryology – ICSI, Fert checks & Embryo grading
• Embryo Biopsy
• Sperm Acrosome assessment using flow cytometry
• Sperm DNA fragmentation
• Genomics- Microarray dataset analysis, Network analyst, PANTHER
• Molecular Biology- Plasmid DNA isolation, cloning of plasmid DNA; Western Blotting
• Primer designing
• Cell culture techniques