Summary
Work History
Education
Skills
Websites
Accomplishments
Additional Information
Languages
Timeline
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MALLAR DASGUPTA

MALLAR DASGUPTA

Kolkata

Summary

Skilled multitasker with superior work ethic and good teamwork, problem-solving and organizational skills. Willing to take on any task to help team. Reliable and dedicated team player with hardworking and resourceful approach.

Work History

Research Internship

Department of genetics, Ballygunge Science College, Calcutta University

This research internship was based on the screening of the ATP7B gene for mutations in Wilson's disease (WD) patients. Wilson's disease is primarily a genetic disorder of copper metabolism caused by a mutation in the ATP7B gene, which generally affects people of variable age groups with diverse hepatic and neurological symptoms. The project comprised of:

a) Collection of blood samples of WD patients (from Bangur Institute of Neurosciences, Kolkata)

b) Genomic DNA isolation

c) PCR amplification of genomic DNA and analyzing PCR products on PAGE gel.

d) Study of homozygous/heterozygous mutations in exons of ATP7B by analyzing the sequencing data in chromatogram; and correlation of respective mutation patterns with the phenotype (Brain MRI scan) of the patients. Within this course, I also learned how to analyze MRI scans, how to calculate cognitive scores (ACE and FAB), and how to detect mutations (Splicing and non- splicing) through the help of various software like mutation tester, Polyphen-2, SNPs & GO, SIFT, FATHMM, Splice AI, ESE 3.0, etc.

Master’s Dissertation

Indian Institute of Technology, Indore

Abstract/ Overview: This project is primarily based upon exploration of certain suitable methods for Salmonella typhimurium OmpA antigen delivery as suitable vaccine candidates. My work encompasses two different antigen delivery platforms to be looked at:


(1) Nanoparticle-mediated encapsulation: which involves encapsulation of OmpA with chitosan nanoparticle and characterization with functional studies to find out the in-vitro release and immunogenicity of the protein. Direct administration of antigens has several disadvantages,

including low immunogenicity and need for multiple dose administration to elicit a sufficiently strong immune response. The nature of subunit antigen or purified antigen is toxic due to non- biodegradable nature and also it provides a considerable level of immune response with high dose. Nanoparticle encapsulation addresses this issues by providing benefits such as low dosage to induce similar immune response, eliminating the need of multiple doses, enhancing immunogenicity, enhanced circulation in bloodstream and preferential uptake by Antigen presenting cells(APCs), controlled release in bloodstream and modulating immune responses.

Being made up of polymeric based substances, one significant advantage is their biodegradable, non-toxic nature in human environment.


(2) Engineered Outer membrane vesicles: When gram-negative microbes undergo stressful conditions, they secrete these outer membrane vesicles in response to environment. They are spheroid vesicles of size 10-300 nm naturally enriched with immuno-active components, including LPS, nucleotide acids, lipids, outer-membrane proteins (OMPs), periplasmic proteins, inner membrane proteins and cytoplasm proteins. Some of these components serve as pathogen-associated molecular patterns (PAMPs) The OMVs play crucial role in nutrient uptake, biofilm formation and cellular pathogenesis. This part of the project involves (i) Cloning and isolation of OMVs containing gene of interest OmpA (ii) Characterization and functional studies.


Thus, both parts of the project aim to evaluate the different ways of Salmonella antigen delivery in eliciting a protective immunity response.

Bachelor’s Dissertation

St. Xavier’s College (Autonomous), Kolkata

The project comprised of a thorough literature study and the writing of a report in review paper format. Klebsiella pneumoniae is a gram-negative pathogen, which supports biofilm formation due to the release of certain signaling molecules called autoinducers, by a mechanism called quorum sensing. Most Gram-negative microbes maintain inter-species communication via LuxI- LuxR system, where LuxI produces N-acyl homoserine lactones (AHLs) and LuxR produces an orphan cognate receptor SdiA, which is not synthesized by Enterobacter sp. This review establishes SdiA as a double-edged sword and explores both the role of SdiA in AHL (+) condition to maintain AI-2 communication and biofilm formation and the role of SdiA in AHL (-) condition to repress thr fimbriae expression and stopping biofilm formation. This consequently leads to an interesting revelation that inter-species cell-to-cell rapport is possible by AI-1 and AI-2, which can further help in short-range horizontal gene transfer mechanisms.

Education

Class 10th -

Patha Bhavan School, WBBSE

Class 12th - undefined

DPS Ruby Park, CBSE

Bachelor’s - undefined

St. Xavier’s College (Autonomous), Kolkata, Calcutta University

Master’s - undefined

Indian Institute of Technology, Indore, Indian Institute of Technology, Indore

Skills

    PCR

    Agarose gel electrophoresis

    SDS-PAGE

    Streak plating and media preparation

    Nanoparticle synthesis (By ionic gelation method)

    UV-VIS spectrophotometry

    Fluorimetry

    Column-based protein purification

    Absorbance estimation using nanodrop/microplate reader

    Sonication

    Membrane filtration

    Steps of cloning

    Cell culture

    Dynamic light scattering (DLS)

    Auto dock tools (Molecular docking)

    Scanning Electron microscopy (SEM)

    Nuclear magnetic resonance (NMR)

    Isothermal colorimetry (iTC)

    Lyophilization

    Western Blotting

    Bioprinting technology (From a hands-on workshop in IIT Indore)

Accomplishments

    1) Received 2nd position in SN Bose Science fair (Vigyan Mela) for successful demonstration of the eco-friendly project: “Reduce, Reuse and Recycle: Ways of combating pollution”

    2) Received 2nd position for giving a successful mini-presentation on Modern Trends in Microbiology, Chap XVII on the topic of “Snake venom peptide, an emerging warrior against biofilm-forming skin bacterium Staphylococcus epidermidis “

    3) Received 2nd position for successful demonstration of the project “Reduce, Reuse and Recycle: Ways of combating pollution” in the Inter-College Model-making competition EUREKA, a part of SIGMA, the annual science fest of St. Xavier’s College, Kolkata organized by SXC Science Association.

    4) Submission of a solution to the problem statement of “Analytical procedure to determine phenol content using the catabolic pathway of a dmp reporter gene in Pseudomonas sp. Strain CF600” in Gujarat Biotechnology University (GBU) Ideathon.

    5) Member of the content team, Industrial and Research Conclave (2022-2023), and Academic Council, IIT Indore.

    6) Member of PG advisory committee of EBSB (Ek Bharat Swachh Bharat) club, IIT Indore.

Additional Information

Course/Workshops done:

1) Attended a 2-day hands-on training program on Biotechnology activities from Birla Industrial and Technological Museum (BITM), Kolkata.

2) Attended and completed a 2-month course organized by the London School of Hygiene and Tropical Medicine (LSHTM) and Liverpool School of Medicine

Topic: Vector Borne diseases and how to control them

3) Completed a 2-month online internship at the Indian Institute of Technology (IIT) Indore. Topic: Helicobacter pylori and Epstein-Barr Virus mediated gastric and oral cancer progression.

4) Completed a 1-month online internship at Vidyasagar University, West Bengal

Topic: Thermal ecology, environment, communities, global change, and runaway collapse in ecosystems.

5) Successful completion of one month project under the guidance of Dr. Anindya Sundar Panja, Postgraduate Department of Biotechnology, Oriental Institute of Science and Technology, Burdwan

Topic: Ricinus communis leaf fluorescence analysis: an essential ethnomedicinal Plant

6) Completed an internship as a research analyst in HAAS School of Business, University of California, Berkeley.

Skills learned: data handling, analytics, visualization, and representation of huge data sets using statistics.

7) Participated in the Technological Fest of IIT Indore, Techno-Samwad, and gave a presentation on the topic of Epigenetics: The principle, causes, and effects of changes.

8) Attended the online workshop of Frontiers in Biological Sciences (FIBS) regarding the assessment of the COVID-19 pandemic.

9) Attended a webinar on Pandemics and Natural disasters: Understanding and coping” organized by the Internal Quality Assurance Cell (IQAC) and Academic Council.

10) Attended the offline science fest SIGMA, organized by St. Xavier’s College Science Association. (SXCSA)

11) Attended a 5-days workshop on methods in Cell and Molecular Biology organized by the Indian Institute of Science (IISC)

12) Attended an online seminar on wine preparation and making organized by Yuksom Breweries, Sikkim.

13) Attended the workshop on “Role of Youth as Peacemakers “organized by NSS.

14) Attended the workshop on “Role of Youth in Bridging the Gap against COVID-19” organized by NSS.

15) Attended the workshop on “Role of Youth in Child Protection” organized by NSS.

16) Attended the lecture session on bio-inorganic chemistry organized by the St. Xavier’s College

, Department of Chemistry (as a part of DBT Star College).

17) Completed an offline hands-on training on biological techniques, organized by the Burdwan Institute of Management and Computer Sciences (BIMS) and Oriental Institute of Science and Technology. (OIST)

18) Attended a seminar on computer-aided drug design by Schrodinger in IIT Indore.

19) Attended a seminar on Modelling and Simulation with MATLAB and Simulink, organized by collaboration of Mathworks and the Academic council, IIT Indore.

20) Attended a seminar on “Integrating computational approaches and experimental data for understanding binding affinity of protein-protein complexes” by Prof. Michael Gromiha, IIT Madras.

21) Attended a seminar on “Extracellular Vesicles associated miRNAs in action: Mechanism of packaging, transport and delivery” by Prof. Subhendra Nath Bhattacharyya, from Indian Institute of Chemical Biology (IICB), Kolkata

22) Attended a scientific talk by Dr. Dhananjay Chaturvedi from CSIR-Centre for Cellular and Molecular Biology (CCMB)

Topic: Muscle Repair and Homeostasis: Insights in vivo

23) Attended a workshop on Proteomics, Single Cell Analysis, and SPR Technology by collaboration with Cytiva and IIT Indore.

24) Attended a seminar by Dr. Prashant Singh Chauhan in IIT Indore.

Topic: Advancements in 3D Bioprinting (talk followed by 3D Bioprinter demonstration/ training)

25) Attended a seminar by Dr. Dhananjay Patankar, an Independent Biopharmaceutical Professional Topic: Bridging the Gap: Academia to Biopharma Industry

26) Attended a talk by Prof. Ganesh Nagaraju (IISC Bangalore)

Topic: RAD51 paralogs: Expanding roles in genome maintenance and tumor suppression.


Academic scholarships received:

• Recipient of University Grant’s Commission-Junior Research Fellow (UGC-JRF) Fellowship (AIR-134).

• Recipient of Department of Biotechnology (DBT) Fellowship for Master’s project.

• Recipient of Fr. Eugene Lafont fellowship for academic excellence in St. Xavier’s College (Autonomous), Kolkata.


Exams qualified:

• IELTS = 8.0 (Listening = 8.5, Reading = 8.5, Writing = 7.0, Speaking = 7.5)

• IIT Joint Admission test (AIR 129)

• DBT- Graduate Aptitude Test Biotechnology (AIR 33)

• Tata Institute of Fundamental Research (Qualified for interviews)

• COGJET (For Cognitive sciences)

• Indian Institute of Science (for Interviews)

• Indian Association for Cultivation of Science (AIR 2)

• GATE Biotechnology (AIR-49)

• GATE Life Sciences (AIR-42)

Languages

Bengali
Bilingual or Proficient (C2)
English
Upper intermediate (B2)
Hindi
Upper intermediate (B2)

Timeline

Research Internship

Department of genetics, Ballygunge Science College, Calcutta University

Master’s Dissertation

Indian Institute of Technology, Indore

Bachelor’s Dissertation

St. Xavier’s College (Autonomous), Kolkata

Class 10th -

Patha Bhavan School, WBBSE

Class 12th - undefined

DPS Ruby Park, CBSE

Bachelor’s - undefined

St. Xavier’s College (Autonomous), Kolkata, Calcutta University

Master’s - undefined

Indian Institute of Technology, Indore, Indian Institute of Technology, Indore

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MALLAR DASGUPTA