Manali Sasane
Thane
Summary
Highly-motivated employee with desire to take on new challenges. Strong work ethic, adaptability, and exceptional interpersonal skills. Adept at working effectively unsupervised and quickly mastering new skills.
Overview
2
2
years of professional experience
1
1
Certification
Work History
R and D Project Lead (Molecular Biology)
Himedia Laboratories
Thane
2021.08 - 2023.05
- Development of Infectious organisms’ detection Kits with SyBr, Probe, or Semiq PCR.
- Optimization of various parameters (Primer and probe conc, DNA conc) and thermal cycling conditions required for PCR.
- Standardization of optimized protocol of detection kits with clinical samples.
- Manual and Automated extraction of Nucleic acids, Quantification and Gel analysis.
- Designing of Primers, Probes and Synthetic DNA using NCBI BLAST and Net Primer software.
- Quality Control testing of raw material, filling, packaging, labeling and dispatching along with MSDS and trasport guidelines.
- involved in regulatory activities that includes maintaining QMS, QA documentation of the data. application of CDSCO application, preparing research file and technical data file for CE-IVD application.
- Maintained accurate records of all project activities including changes, milestones achieved.
- Created project performance reports for management review.
- Analyzed customer requirements in order to develop solutions that meet their needs.
- Provided support for troubleshooting during system development process.
Molecular Laboratory Technician
Panacea Laboratory
Mumbai
2021.01 - 2021.08
- Executing and performing routine analysis of CoVID test using CRISPR Cas9 developed kits. Developed by the TATAMD group
- Documentation procedure, writing SOPs., Inter laboratory Comparison activities.
- Maintained detailed records of test results for review by supervisors or other personnel as required.
- Maintained laboratory equipment by performing calibration checks and troubleshooting any malfunctions.
- Provided technical support to colleagues regarding methods used in experiments or data interpretation.
- Resolved customer complaints relating to laboratory services in a timely manner.
- Identified potential problems during testing process and took corrective action as necessary.
- Assisted with training new lab technicians on proper operation of instruments, safety protocols, and other relevant topics.
- Monitored safety compliance within the laboratory environment to ensure adherence to regulations.
Education
Ph.D. - Life Sciences
Mumbai University
Mumbai2024-05
Master of Science - Biotechnology
Mumbai University
Mumbai2013-04
Bachelor of Science - BIotechnology
Mumbai University
Mumbai2011-04
Skills
- Project Management
- Project Documentation
- Project Development
- Quality Control
- Report Writing
- Quality Assurance
TECHNICAL SKILLS
- Ms Office: Competent in Word, Excel and PowerPoint.
- Molecular Software: Familiar with NCBI primer desinging, ClustalW, UPGMA,
- Statistical Software: Fundamental knowledge of Graphpad Prism, Image J, Real Time machine Software.
LABORATORY SKILLS
- PLANT TISSUE CULTURE: Media standardisation, micropropagation callus and cell suspension culture.
- MOLECULAR BIOLOGY: DNA and RNA extraction, Real time PCR, Sequencing, Primer Designing, AGE and Gel Analysis
- BIOCHEMISTRY: Enzymatic and biochemical assay.
- INSTRUMENTATION: Fundamental knowledge and Handling experience of HP-TLC, HPLC, UV-VIS spectrophotometer, MIcrotiter Plate, Reader, PCR, Real time PCR, Gel-Doc Image Analyser.
- ANIMAL TISSUE CULTURE: Cell line maintenance and passaging, Cytotoxicity testing, MTT Assay.
- MICROBIOLOGY: Antimicrobial Assay, MIC determination.
Certification
- Completed ISO 15189:212 for the Medical Testing laboratory training program by Medical Education and Learning Point (MElAP).
Affiliations
- Presented poster on Covid-19 detection kit validation at EECMID, Portugal (2022).
- Worked as a visiting faculty lecturer for UG and PG Biotechnology.
- Cleared PhD Entrance Examination (2014).
- ATREC: Attended workshop on Basic techniques in Understanding Epigenetics (2019).
- National Chemical Laboratory: Attended workshop on Plant tissue culture techniques, molecular profiling, and fingerprinting. (2019).
- Attended Lecture seminar on Research Methodology and Scientific writing (2019).
- Jaihind College: Attended lecture seminar and workshop on neuroscience perception vs. Recognition. (2016).
- Piramal Diagnostics: completed hands-on training on molecular biology techniques (2010)
Languages
Marathi
First Language
English
Intermediate (B1)
B1
Hindi
Intermediate (B1)
B1
References
1) Dr. Indu Anna George
Associate Professor
Dept of Life Sciences, University of Mumbai
Mob:9821477971
2) Dr. Seema Agashe
Assistant Professor
Dept. Of Biotechnology, K.V. Pendharkar college
Mob: 9920014577
3) Dr. Kamlesh Jangid
Scientist
Agarkar Research Institute
Mob: 956110781
Timeline
R and D Project Lead (Molecular Biology)
Himedia Laboratories
2021.08 - 2023.05
Molecular Laboratory Technician
Panacea Laboratory
2021.01 - 2021.08
Ph.D. - Life Sciences
Mumbai University
Master of Science - Biotechnology
Mumbai University
Bachelor of Science - BIotechnology
Mumbai University
- Completed ISO 15189:212 for the Medical Testing laboratory training program by Medical Education and Learning Point (MElAP).
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