Manali Sasane
Thane
Summary
Highly-motivated employee with desire to take on new challenges. Strong work ethic, adaptability, and exceptional interpersonal skills. Adept at working effectively unsupervised and quickly mastering new skills.
Overview
2
2
years of professional experience
1
1
Certification
Work History
R and D Project Lead (Molecular Biology)
Himedia Laboratories
Thane
08.2021 - 05.2023
- Development of Infectious organisms’ detection Kits with SyBr, Probe, or Semiq PCR.
- Optimization of various parameters (Primer and probe conc, DNA conc) and thermal cycling conditions required for PCR.
- Standardization of optimized protocol of detection kits with clinical samples.
- Manual and Automated extraction of Nucleic acids, Quantification and Gel analysis.
- Designing of Primers, Probes and Synthetic DNA using NCBI BLAST and Net Primer software.
- Quality Control testing of raw material, filling, packaging, labeling and dispatching along with MSDS and trasport guidelines.
- involved in regulatory activities that includes maintaining QMS, QA documentation of the data. application of CDSCO application, preparing research file and technical data file for CE-IVD application.
- Maintained accurate records of all project activities including changes, milestones achieved.
- Created project performance reports for management review.
- Analyzed customer requirements in order to develop solutions that meet their needs.
- Provided support for troubleshooting during system development process.
Molecular Laboratory Technician
Panacea Laboratory
Mumbai
01.2021 - 08.2021
- Executing and performing routine analysis of CoVID test using CRISPR Cas9 developed kits. Developed by the TATAMD group
- Documentation procedure, writing SOPs., Inter laboratory Comparison activities.
- Maintained detailed records of test results for review by supervisors or other personnel as required.
- Maintained laboratory equipment by performing calibration checks and troubleshooting any malfunctions.
- Provided technical support to colleagues regarding methods used in experiments or data interpretation.
- Resolved customer complaints relating to laboratory services in a timely manner.
- Identified potential problems during testing process and took corrective action as necessary.
- Assisted with training new lab technicians on proper operation of instruments, safety protocols, and other relevant topics.
- Monitored safety compliance within the laboratory environment to ensure adherence to regulations.
Education
Ph.D. - Life Sciences
Mumbai University
Mumbai05-2024
Master of Science - Biotechnology
Mumbai University
Mumbai04-2013
Bachelor of Science - BIotechnology
Mumbai University
Mumbai04-2011
Skills
- Project Management
- Project Documentation
- Project Development
- Quality Control
- Report Writing
- Quality Assurance
TECHNICAL SKILLS
- Ms Office: Competent in Word, Excel and PowerPoint
- Molecular Software: Familiar with NCBI primer desinging, ClustalW, UPGMA,
- Statistical Software: Fundamental knowledge of Graphpad Prism, Image J, Real Time machine Software
LABORATORY SKILLS
- PLANT TISSUE CULTURE: Media standardisation, micropropagation callus and cell suspension culture
- MOLECULAR BIOLOGY: DNA and RNA extraction, Real time PCR, Sequencing, Primer Designing, AGE and Gel Analysis
- BIOCHEMISTRY: Enzymatic and biochemical assay
- INSTRUMENTATION: Fundamental knowledge and Handling experience of HP-TLC, HPLC, UV-VIS spectrophotometer, MIcrotiter Plate, Reader, PCR, Real time PCR, Gel-Doc Image Analyser
- ANIMAL TISSUE CULTURE: Cell line maintenance and passaging, Cytotoxicity testing, MTT Assay
- MICROBIOLOGY: Antimicrobial Assay, MIC determination
Certification
- Completed ISO 15189:212 for the Medical Testing laboratory training program by Medical Education and Learning Point (MElAP).
Affiliations
- Presented poster on Covid-19 detection kit validation at EECMID, Portugal (2022).
- Worked as a visiting faculty lecturer for UG and PG Biotechnology.
- Cleared PhD Entrance Examination (2014).
- ATREC: Attended workshop on Basic techniques in Understanding Epigenetics (2019).
- National Chemical Laboratory: Attended workshop on Plant tissue culture techniques, molecular profiling, and fingerprinting. (2019).
- Attended Lecture seminar on Research Methodology and Scientific writing (2019).
- Jaihind College: Attended lecture seminar and workshop on neuroscience perception vs. Recognition. (2016).
- Piramal Diagnostics: completed hands-on training on molecular biology techniques (2010)
Languages
Marathi
First Language
English
Intermediate (B1)
B1
Hindi
Intermediate (B1)
B1
Timeline
R and D Project Lead (Molecular Biology)
Himedia Laboratories
08.2021 - 05.2023
Molecular Laboratory Technician
Panacea Laboratory
01.2021 - 08.2021
Ph.D. - Life Sciences
Mumbai University
Master of Science - Biotechnology
Mumbai University
Bachelor of Science - BIotechnology
Mumbai University