Summary
Overview
Work History
Education
Skills
Accomplishments
Core Competency
Domain Specific Skills
Hobbies and Interests
Training
Languages
Disclaimer
Timeline
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Medha Sudarshan

Medha Sudarshan

Bengaluru

Summary

A dedicated Research Professional with three years of experience in Next Generation Sequencing (NGS) and Molecular Biology, committed to advancing the field of NGS research. Driven by a passion for Biotechnology, I aim to continuously expand my expertise and skill set, contributing to innovative solutions and advancements. My enthusiasm for research tools and data analytics in Biosciences fuels my desire for both personal and organizational growth.

Overview

Work History

Senior Research associate, NGS Department

Syngene International Pvt Ltd
Bengaluru
10.2024 - Current
  • Optimized and executed V-gene Library preparation and sequencing of mouse hybridoma cells by NGS
  • Optimized the library preparation for V-gene sequencing of mouse single cell
  • Worked on RNA extraction, V-gene sequence amplification and Library preparation from rabbit single cell
  • Performed Amplicon sequencing for several projects using NGS platform
  • Worked on targeted sequencing for a project on knock-out mice generation
  • Successfully executed Molecular cloning and scale-up for various projects
  • Conducted gene expression studies using qPCR technique.

Research associate, NGS Department

Syngene International Pvt Ltd
Bengaluru
08.2024 - 10.2024
  • Performed whole genome bacterial sequencing, scRNA sequencing
  • Performed CD 16 Genotyping experiments for SNP studies
  • Executed HLA typing sequencing runs
  • Performed several Molecular cloning experiments and well versed with molecular biology techniques
  • Worked on an Assay Development Project involving the Optimization of different Assays to test the commercial viability of in-house synthesized Taq Polymerase and Reverse Transcriptase enzymes
  • Performed RNA Isolation from various mouse tissue samples
  • Outlined key research output in documents, spreadsheets, and reports
  • Used databases, physical records, and digital resources to complete deep research
  • Maintained inventory, equipment, and materials in an organized manner
  • Have trained peers in various experimental techniques.

Education

Master of Science - Biotechnology

School of Life Sciences, Jain University
01.2021

Bachelor of Science - Biotechnology, Biochemistry, Genetics

Jyoti Nivas (Autonomous) College
01.2019

PUC - PCMB

Sri Kumaran Childrens Home PUC College
01.2016

SSLC -

A V Education Society
01.2014

Skills

  • Report writing
  • Documentation abilities
  • Analyzing using Softwares
  • Data presentation
  • Inventory assessments
  • Effective communication skills
  • Training skills
  • Trouble Shooting and Optimizing

Accomplishments

Business Achievement Award for contributing as a team member in one of the client's projects

Core Competency

  • Expertise in NGS QC (Qubit and Tape Station), Library preparation and NGS Sequencing runs using Miseq instrument
  • Well-versed in Molecular biology techniques like agarose gel electrophoresis, PCR, qPCR, cloning and scale-up

Domain Specific Skills

  • Agarose Gel Electrophorosis
  • Polymerase Chain Reaction
  • QPCR
  • DNA and RNA Extraction
  • Cloning (Digestion-Ligation, Infusion)
  • Cloning Scale-up
  • NGS Sequencing
  • Primer Designing
  • Analyzing Using Graph Pad Prism Sequencing
  • Analysis using Sequencher Software
  • Use of instruments like Qubit, Tapestation, tecan spark plate reader Quant studio, Maxwell RSC Instrument

Hobbies and Interests

Professional dancer

Training

  • Molecular Biology Techniques such as ELISA, Western blotting and SDS PAGE
  • Basics of cell culture and maintaining Cell lines
  • Flow Cytometry ADCC Assay Single Cell experiments using R NADIA Instrument
  • ScRNA sequencing using HIVE

Languages

English
First Language

Disclaimer

The Information provided by me is true and correct to my knowledge and belief

Timeline

Senior Research associate, NGS Department

Syngene International Pvt Ltd
10.2024 - Current

Research associate, NGS Department

Syngene International Pvt Ltd
08.2024 - 10.2024

Master of Science - Biotechnology

School of Life Sciences, Jain University

Bachelor of Science - Biotechnology, Biochemistry, Genetics

Jyoti Nivas (Autonomous) College

PUC - PCMB

Sri Kumaran Childrens Home PUC College

SSLC -

A V Education Society
Medha Sudarshan