Naganathswamy Mandi

Dynamic individual with hands-on experience in cell line development and process development and talent for navigating challenges. Brings strong problem-solving skills and proactive approach to new tasks. Known for daptability, creativity, and results-oriented mindset. Committed to making meaningful contributions and advancing organizational goals. Developed cell lines for four biosimilar mAbs with productivity in the range of 2 - 3.5 g/L. Scaled-up at 2 L and 5 L scales and process optimization for bioreactor studies to achieve the high cell count and high productivity. Successful expression of Fab molecule in periplasmic space in E. Coli and considered for product development. Several research papers published in peer-reviewed journals (National and International).

• ICAR-JRF awarded during Master's program
• CSIR -SRF awarded during Doctoral program

• Asn12 and Asn278: Critical Residues for In-Vitro Biological Activity of Reteplase, Naganath Mandi, Kalyana R. Sundaram, Sunil K. Tandra, Suman Bandyopadhyay, and Sriram Padmanabhan, Advances in Hematology, 2010, 2010
• Construction of a novel zero background prokaryotic expression vector: potential advantages, Naganath Mandi, Prakash kotwal and Sriram Padmanabhan, Biotechnology Letters, 31, 12, 1905-1910, 2009
• High yielding recombinant Staphylokinase in bacterial expression systemcloning, expression, purification and activity studies, Naganath S Mandi, Sudheerbabu Soorapaneni, Sachin Rewanwar, Prakash Kotwal, BhaskarJyoti Prasad, Goutam Mandal and Sriram Padmanabhan, Protein expression and Purification, 64, 1, 69-75, 2009
• Over-expression of proteins using a modified pBAD24 vector in E. coli expression system, Sampali Banerjee, Shardul S. Salunkhe, Anjali D. Apte-Deshpande, Naganath S. Mandi, Goutam Mandal and Sriram Padmanabhan, Biotechnology Letters, 31, 7, 1031-1036, 2009
• A Novel Cytokine Derived Fusion Tag for Over-Expression of Heterologous Proteins in E. coli, Sampali Banerjee, Anjali Apte Deshpande, Naganath Mandi and Sriram Padmanabhan, International Journal of Biological and Life Sciences, 5, 3, 129-133, 2009
• Genetic typing of bovine viral diarrhoea virus isolates from India, Mishra, N., Pattnaik, B., Vilcek, S., Patil, S.S., Swamy, N., Bhatia, S and Pradhan, H.K., Vet Microbiol., 104, 3-4, 207-12, 2004
• Characterization of 59 kDa gelatin binding fragment of buffalo plasma fibronecetin, Nizamuddin Ahmed and Naganath Swamy, Indian Journal of Biochemistry and Biophysics, 39, 113-118, 2002
• Beta-defensin antibiotic peptides in the innate immunity of the buffalo: in vivo and in vitro studies, Das H, Swamy N, Sahoo G Ahmed SU, More T, Altern Lab Anim., 36, 4, 429-40, 2008
• Screening of Bacterial Recombinants: Strategies and Preventing False Positives, Sriram Padmanabhan, Sampali Banerjee, and Naganath Mandi, Molecular Cloning - Selected Applications in Medicine and Biology, Gregory G Brown, INTECH, 2011

• Training on mammalian cell line development for Bioptherapeutics at NeuClone Pty Ltd, Sydney, Australia
• Training on Six sigma [Green belt]

• Lethal Gene as a tool for zero background cloning, Naganath Mandi, Shardul Salunkhe and Sriram Padmanabhan, WO/2010/018592 A2, 02/18/10, PCT/IN2009/000377
• Expression of heterologous proteins in bacterial system using a GM-CSF fusion tag, Sampali Banerjee, Anjali Apte-Deshpande, Naganath Mandi and Sriram Padmanabhan, WO/2010/001414 A1, 01/07/10, PCT/IN2009/000372