Pallavi
Zirakpur
Summary
Highly-motivated employee with desire to take on new challenges. Strong work ethic, adaptability, and exceptional interpersonal skills. Adept at working effectively unsupervised and quickly mastering new skills.
Overview
4
4
years of professional experience
Work History
Research Associate
Aurigene Oncology Limited
04.2024 - Current
- Performing regular sample analysis for In-process, Harvest and FPAC stages using Flowcytomtery, Cytotoxicity and PCR based assays.
- Validation of IFNgamma ELISA, CD348 and Cell count.
- Release of Critical raw materials used for production usage using 5 day potency assay that mimics the production process.
- Preparation and maintenance of data trends.
- Regular monitoring and Lab management.
- Revival, subculture and cryopreservation of cells.
- Performing Cytokine profiling and Sear horse assay to test the cytokine profile of effector cells and their persistence.
- Preparation and review of SOPs, STPs, Validation protocols and reports.
- Handling of QMS elements.
Officer
Lupin Biotech
03.2023 - 03.2024
- Worked on Mabs and protein molecules.
- Performing regular sample analysis using potency based assays, cellbased assay, ELISA and RT-PCR.
- Involved in Validation of Binding ELISA for Ranibizumab.
- Regular maintenance of cell-lines. Subculture and Revial of cell lines.
- Validation of RT-PCR based method for Quantitation of HC-DNA in Denosumab.
- Performing cell based bioassay for Etanercept, Ranibizumab, Aflibercept and Pegfilgrastim.
- Preparation of reports, SOPs, Method validation protocols.
- Handling of QMS elements.
Associate
Stelis Biopharma limited
Zirakpur
08.2021 - 03.2023
- Performing regular sample analysis by Cell-culture(TCID50), ELISA, PCR based assays
- TCID-50 (Cell-culture based viral titer testing): Passaging, maintaining of cell line
- Preparation of Media for cells, Regular monitoring of cells for morphology checks and viral infection, and testing of viral titer
- ELISA: Determination of host cell proteins, residual enzyme, potency binding assay.
- PCR Based: Extraction of viral DNA, detection by RT-PCR, Mycoplasma contamination determination of PCR
- Method transfer of HCP, TCID50 and Potency ELISA.
- Preparation of SOPs, reports for validation and documents regarding testing and assay execution.
- Decontamination: Extraction of Viral DNA using kit based method and Quantitation using RT-PCR.
- Potency based Assay: Optimising the concentration of secondary antibody and other parameters in assay.
Education
Master of Science - Biotechnology
Panjab University
Chandigarh01.2021
Bachelor of Science - Biotechnology
Panjab University
Chandigarh01.2019
Skills
- Cell culture
- Method validation
- Quality management systems
- Critical raw material release
- Problem solving
- QPCR
- Flow cytometry
- Prism
Accomplishments
New Technology Trailblazer
Timeline
Research Associate
Aurigene Oncology Limited
04.2024 - Current
Officer
Lupin Biotech
03.2023 - 03.2024
Associate
Stelis Biopharma limited
08.2021 - 03.2023
Master of Science - Biotechnology
Panjab University
Bachelor of Science - Biotechnology
Panjab University