Paloma Dutta
Molecular Biologist
Berhampore
Summary
Enthusiastic, Motivated, Diligent and Versatile. Holding degree of Master of Science in Molecular Biology & Biotechnology Stream. Excellence in Communication, team work, scientific operations. Well versed in different PCR, Sanger sequencing, NGS, Animal cell culture Western Blotting, HPLC. Skilled at analyzing, multitasking and troubleshooting.
Overview
7
7
years of professional experience
16
16
years of post-secondary education
1
1
Certification
3
3
Languages
Work History
Senior Molecular Technician
Center of Advanced Research on Diagnostics in Cancer(C-CARD), Karkinos Healthcare, IIT Guwahati
- Current
- Development and implementation of SOPS for molecular oncology lab
- Initiated and standardized of RT PCR and sequence-based assay for cancer diagnosis
- Performing Whole Genome Sequencing, targeted gene panel sequencing in Illumina Novaseq and Nextseq Sequencer
- Laboratory inventory management as per institute inventory management policy.
Scientific Officer
Tata Medical Center, Kolkata
01.2021 - 02.2023
- My project involved in standardization of different Real Time PCR, DDPCR assay for molecular oncology test development
- Performing routine DNA and RNA extraction from blood and FFPE tissue, RT-PCR, Fragment Analysis, Sanger sequencing, NGS of clinical samples
- Responsible for interpretation and implementation of established procedures to prepare appropriate specimens for cytogenetic and FISH analysis.
Junior Research Fellow
Amity University KOLKATA
09.2018 - 12.2019
- Identification of differentially expressed genes during EMT transition and to understand the physiological significance of those changes
- Assessment of different EMT markers expression at both RNA and protein level is done using NMUMG cells as a model system
- Analysis of RNAseq data which intoned changes at the transcriptional level during induction of EMT by TGF-beta exposure
- The RNAseq data was then further validated by real time PCR analysis and expression of those genes at protein level also checked by Western Blotting.
Research Assistant
IISER-KOLKATA
02.2018 - 08.2018
- Project involved to characterize the IP6 mediated dimer of BTK
- In this project, I have performed protein (BTK) purification using NI-NTA column by HPLC method
- After purifying protein, I have done Gel filtration assay for deducing the interaction between BTK and IP6.
Summer Intern
CSIR-IICB
05.2017 - 06.2017
- Dissertation named 'Ellagic acid induces ROS generation caused anti-proliferation of colon carcinoma cells (HCT-116)- in vitro study'
Education
Master Of Science - Molecular biology & Biotechnology
University of Kalyani
Kalyani 08.2015 - 07.2017
Bachelor of Science - Zoology
Krishnath College, University of Kalyani
04.2001 - 04.2015
Higher Secondary (10+2) -
B.M.K.G.H.S., WBCHSE
School Final (10th Standard) -
B.M.K.G.H.S., WBBSE
West BengalSkills
PCR
NGS
Cell culture
Western Blot
HPLC
FISH
DNA / RNA isolation
Sanger Sequencing
Protein purification
Light Microscopy
Microbial culture
Chromosome analysis
Accomplishments
- GATE (Life science) - 2017, 2019
- TIFR-JGGEBILS - 2017,2019
Personal Information
- Blood Group: B +ve
- Date of Birth: 10/08/94
- Nationality: Indian
- Marital Status: Single
References
- Dr. Deepak Kumar Mishra, Senior Consultant, Lab Hematology & Molecular Genetics, Department of Laboratory Services, TATA Medical Center, Newtown, Kolkata, 700160, deepak.mishra@tmckolkata.com
- Dr. Santanu Palchaudhuri, Assistant Director, AIBNK, Amity University Kolkata, Newtown, Kolkata, 700156, santanu.palchaudhuri@gmail.com
- Dr. Rahul Das, Assistant Professor, Department of Biology, IISER KOLKATA, Kalyani, Kolkata, West Bengal, 741246, rahul.das@iiserkol.ac.in
Certification
[Cancer Genomics], [Decode] - [One month]
Timeline
[Cancer Genomics], [Decode] - [One month]
09-2023
Scientific Officer
Tata Medical Center, Kolkata
01.2021 - 02.2023
Junior Research Fellow
Amity University KOLKATA
09.2018 - 12.2019
Research Assistant
IISER-KOLKATA
02.2018 - 08.2018
Summer Intern
CSIR-IICB
05.2017 - 06.2017
Master Of Science - Molecular biology & Biotechnology
University of Kalyani
08.2015 - 07.2017
Bachelor of Science - Zoology
Krishnath College, University of Kalyani
04.2001 - 04.2015
Senior Molecular Technician
Center of Advanced Research on Diagnostics in Cancer(C-CARD), Karkinos Healthcare, IIT Guwahati
- Current
Higher Secondary (10+2) -
B.M.K.G.H.S., WBCHSE
School Final (10th Standard) -
B.M.K.G.H.S., WBBSE