Passong Immanual R Chhakchhuak
Hyderabad,TG
Summary
As a dedicated research professional passionate about interdisciplinary research. My expertise includes transcription biology, RNA biology, E. coli genetics and molecular biology, honed through rigorous training and hands-on experience. I approach problems from multiple perspectives, and developing comprehensive solutions.
I am detail-oriented, methodical, and efficient in experimental design and execution, ensuring reliable results. I seek a full-time position that offers professional challenges and growth opportunities.
I bring strong organizational skills and problem-solving abilities. I am eager to help the team achieve company goals and confident in my ability to make a positive impact as a team member.
Overview
10
10
years of professional experience
Work History
Research Associate II
Centre For DNA Fingerprinting And Diagnostics
04.2024 - Current
- Initiated the investigation into transcription termination machinery of Bacillus subtilis.
- Monitored progress against project timelines, ensuring timely completion of deliverables while maintaining high-quality standards.
- Provided technical guidance on experimental design and data interpretation issues for team members as needed.
- Gathered, arranged, and corrected research data to create representative graphs and charts highlighting results for presentations.
- Conducted comprehensive literature reviews to inform study designs and methodology decisions.
Research Associate
Indian Association for the Cultivation of Science
07.2023 - 03.2024
- Set up equipment, organized inventory and maintained facilities.
- Provided technical guidance on experimental design and data interpretation issues for team members as needed.
- Conducted comprehensive literature reviews to inform study designs and methodology decisions.
- Collaborated with multidisciplinary teams to drive project.
- Explored Bacillus subtilis transcription termination machinery.
Research Scholar
Centre For DNA Fingerprinting And Diagnostics
06.2016 - 10.2023
- Participated in multiple projects related to the function of E. coli transcription termination factor Rho.
- Evaluated statistical models for validity and reliability, ensuring accurate interpretation of research outcomes.
- Enhanced understanding of complex phenomena through advanced quantitative and qualitative data analysis techniques.
- Presented findings at prestigious conferences, sharing valuable insights with international audiences and building professional relationships among peers.
- Mentored junior researchers, providing guidance on study design, data analysis, and manuscript preparation for successful submissions to journals.
- Conducted comprehensive literature reviews to identify gaps in existing knowledge and inform future research directions.
- Developed new research methodologies that streamlined data collection processes, resulting in increased efficiency and accuracy of results.
M.Sc. Dissertation
MKU, Madurai
10.2014 - 08.2015
- Construction of gus hair-pin RNA (hpRNA), mobilization into Agrobacterium
- Construction of gus hpRNA for studying gene silencing
- This work is for further studies in plant pathogen relationship between Rhizoctonia solani and rice.
Education
Doctor of Philosophy (Biotechnology) -
Centre for DNA Fingerprinting and Diagnostics
07.2023
Master of Biotechnology -
Madurai Kamaraj University
07.2015
Bachelor of Biotechnology -
St. Anthony’s College (affiliated with N.E.H.U)
06.2013
Skills
- Microbiology and aseptic techniques
- PCR-based technologies- PCR, PCR-based SDM, cDNA preparation etc
- Basic biochemistry- PAGE, western and southern blots
- Transformation, transduction (phage-based P1 and λ transduction), linear DNA electroporation, Agrobacterium-mediated transformation
- Gene expression analysis- Real-Time -PCR experiments (semi-quantitative and quantitative)
- Cell-based assays/enzymatic assays- β-gal assay, ATPase assay (malachite green and radioactive ATPase assay)
- Random mutagenesis and setting up screens for selection
- In vitro based assays- in vitro transcription, in vitro transcription assay, Electrophoresis Mobility Shift Assay (EMSA), and Reverse Transcription
- Identification of novel genes from public databases (EST, Genomic and Protein databases) using programs and search tools like CLUSTAL W, BLAST, etc
- Sequence analysis of PCR products/genes generated by ABI
- Familiar with genome databases like Ecocyc, NCBI, etc
- Theoretical knowledge practical skills in genetics, genomics, molecular biology, genetic engineering, and biochemistry
- Basic knowledge of Python
- Working knowledge of Pymol
Research
- Research Scholar, CDFD, Hyderabad, 06/2016, Present, Microbial Biochemistry and Transcription Biology. Participated in multiple projects related to the function of E. coli transcription termination factor Rho. Studied the role of nascent RNA in the regulation of Rho-dependent transcription termination. Studied the modulation of Rho-dependent transcription termination by termination factor NusG. Studied the functional interaction sites between Rho and NusG. Studied the Rho regulation of toxin-antitoxin modules of cryptic prophages., Bacterial transduction, Reverse transcription, real-time PCR, EMSA, ATPase assay, Molecular Cloning, Microarray, Statistical Analysis, Sequence analysis, β-gal assay, random mutagenesis, basic Python programming, in vitro transcription assay
- M.Sc. Dissertation, MKU, Madurai, 10/2014, 08/2015, Construction of gus hair-pin RNA (hpRNA), mobilization into Agrobacterium. Construction of gus hpRNA for studying gene silencing. This work is for further studies in plant pathogen relationship between Rhizoctonia solani and rice., Molecular cloning, hpRNA Construction, Southern Blot, Agrobacterium mediated transformation
Personal Information
- Date of Birth: 01/22/1991
- Gender: Male
- Nationality: Indian
- Marital Status: Unmarried
Postersandconferences
- NusG-dependent terminators of factor-dependent transcription termination, Passong Immanuel R Chhakchhuak and Ranjan Sen, 20th Transcription Assembly meeting (CDFD, Hyderabad), 07/25/2018
- Exploring the NusG-dependent transcription termination signals of E.coli, Passong Immanuel R Chhakchhuak and Ranjan Sen, 2nd Meeting on Molecular microbiology (CDFD, Hyderabad), 07/10/2019
- Exploring the NusG-dependent transcription termination signals of E.coli, Passong Immanuel R Chhakchhuak and Ranjan Sen, Biological Transactions: From Molecules to Organisms (IISc, Bangalore), 01/17/2019
- In vivo regulation of bacterial Rho-dependent transcription termination by the nascent RNA, Passong Immanuel R Chhakchhuak and Ranjan Sen, Society of Biological Chemists (Sister Nivedita University, Kolkota), 12/08/2022
Awards
- Doctor of Philosophy from Regional Centre for Biotechnology, Faridabad (2023)
- Awarded Department of Biotechnology, India- Junior Research Fellowship (2016)
- Qualified Joint Graduate Entrance Examination for Biology and Interdisciplinary Life Sciences (JGEEBILS) conducted by Tata Institute of Fundamental Research (TIFR) (2015)
- Awarded Department of Biotechnology (DBT), India - CEEB Fellowship Received scholarship for M.Sc (2013)
- MKU, Madurai, Tamil Nadu top 7th rank in M.Sc. Biotechnology
- NEHU, Shillong, Meghalaya top 8th rank in B.Sc. Biotechnology
Publications
- Mechanism of Action of Bacterial Transcription Terminator Rho, Immanuel Chhakchhuak, P. R., Khatri, A., and Sen, R., 85, 157-168, https://doi.org/10.16943/ptinsa/2018/49436, 2018
- Rho-dependent transcription termination regulates the toxin–antitoxin modules of cryptic prophages to silence their expression in Escherichia coli, Hafeezunnisa, M., Chhakchhuak, P. I. R., Krishnakumar, J., and Sen, R., FEBS Lett., 595, 2057–2067, https://doi.org/10.1002/1873-3468.14150, 2021
- In vivo regulation of bacterial Rho-dependent transcription termination by the nascent RNA, Chhakchhuak, P. I. R., and Sen, R., Journal of Biological Chemistry, 298, 102001, https://doi.org/10.1016/j.jbc.2022.102001, 2022
References
- Dr. Ranjan Sen, Scientist VII, Director, Indian Association for the Cultivation of Science, 2A & B Raja S C Mullick Road, Kolkata, West Bengal, 700032, India, +91-33-24734688 (Direct), +91-33-24734971 (Extn:1101), director@iacs.res.in
- Dr. Subhadeep Chatterjee, Scientist VI, Lab Plant-Microbe Interaction, Centre for DNA Fingerprinting and Diagnostics, Inner Ring Road, Uppal, Hyderabad, Telangana, 500039, India, +91-40-27216108, subhadeep@cdfd.org.in
- Dr. Manjula Reddy, Scientist VII, Dean of Academics, Molecular Oncology, Centre for DNA Fingerprinting and Diagnostics, Inner Ring Road, Uppal, Hyderabad, Telangana, 500039, India, +91-40-27216112, bashyam@cdfd.org.in
Timeline
Research Associate II
Centre For DNA Fingerprinting And Diagnostics
04.2024 - Current
Research Associate
Indian Association for the Cultivation of Science
07.2023 - 03.2024
Research Scholar
Centre For DNA Fingerprinting And Diagnostics
06.2016 - 10.2023
M.Sc. Dissertation
MKU, Madurai
10.2014 - 08.2015
Doctor of Philosophy (Biotechnology) -
Centre for DNA Fingerprinting and Diagnostics
Master of Biotechnology -
Madurai Kamaraj University
Bachelor of Biotechnology -
St. Anthony’s College (affiliated with N.E.H.U)