Prachi Tewari

Meticulous, analytical researcher and educator with more than 5 years of educational and hands-on experience in molecular biology, immunology, plant biotechnology. Adaptive team player with in-depth knowledge of data collection, confidential document control and problem-solving.

• PCR Amplification
• Gel Electrophoresis (SDS-PAGE, Western Blotting)
• Cloning
• DNA Isolation
• Plasmid Isolation
• RNA Isolation
• C-DNA Preparation
• Protein purification
• Semiquantitative and Real time PCR
• Flow cytometry
• Si RNA
• Tissue / Cell Culture (primary & cancerous)
• Preclinical cancer model (skin) work and animal handling
• Immunological techniques (MLR, NK cell activity, lymphoproliferation, cytokine analysis etc)
• Biochemical analysis
• Microscopy

• Benzanthrone induced immunotoxicity via oxidative stress and inflammatory mediators in Balb/c mice., Tewari P, Roy R, Mishra S, Mandal P, Yadav A, Chaudhari BP, Chaturvedi RK, Dwivedi PD, Tripathi A, Das M., Immunobiology, 2015, Mar; 220(3):369-81.
• Deoxynivalenol induced mouse skin tumor initiation: Elucidation of molecular mechanisms in Human Hacat keratinocytes., Sakshi Mishra, Prachi Tewari, Bhushan P. Chaudhari, Premendra D. Dwivedi, Haushila P. Pandey, Mukul Das., Int J Cancer, 2016, Nov 1;139(9):2033-46.
• A novel function of TLR4 in mediating the immunomodulatory effect of Benzanthrone, an environmental pollutant., Prachi Tewari, Payal Mandal, Ruchi Roy, Somya Asthana, Premendra D Dwivedi, Mukul Das, Anurag Tripathi., Toxicology Letters, 2017, DOI: 10.1016/j.toxlet.2017.05.008.
• Argemone oil, an edible oil adulterant, induces systemic immunosuppression in Balb/c mice in an oral 28 days repeated dose toxicity study., Mandal P, Tewari P, Kumar S, Yadav S, Ayanur A, Chaturvedi RK, Das M, Tripathi A., Chem Biol Interact, 2018, doi: 10.1016/j.cbi.2018.04.013.
• Troponin 1 of human filarial parasite Brugia malayi: cDNA cloning, expression, purification, and its immunoprophylactic potential., Kushwaha V, Tewari P, Mandal P, Tripathi A, Murthy PK., Parasitol Res, 2019, doi: 10.1007/s00436-019-06316-8.
• Leishmania donovani molecules recognized by sera of filaria infected host facilitate filarial infection., Verma R, Kushwaha V, Pandey S, Thota JR, Vishwakarma P, Parmar N, Yadav PK, Tewari P, Kar S, Shukla PK, Murthy PK., Parasitol Res, 2018, doi: 10.1007/s00436-018-5981-9. Epub 2018 Jun 26.
• Immune Response Profiling of Cocktails of Brugia malayi Vaccine Candidates DIM-1, Calponin and Troponin 1 in BALB/c Mice., Murthy PK, Tewari P, Mandal P, Kushwaha V., Acta Parasitol, 2023, Dec;68(4):929-936. doi: 10.1007/s11686-023-00725-7.

• National conference on Impact of Environmental Xenobiotics on Human Health and Biodiversity, 03/30/18 - 03/31/18, Second best poster presentation, Benzanthrone, a textile dye intermediate, induces immunosuppression by causing mitochondrial dysfunction and activating DNA damage response pathways.
• International conference on Nature, Science and Modern Lifestyle, Best oral presentation

• Research Associate, Department of Zoology, Lucknow University, Lucknow, 06/2017 - 12/2019, Molecular characterization of immunomodulatory protein(s) of human filarial parasite Brugia malayi
• Project Assistant, National Botanical Research Institute (NBRI), Lucknow, 03/2009 - 09/2010, Functional analysis of novel petal abscission and senescence related genes from Rose and Gladioli