Pradeep Bist

Dynamic Researcher with a Ph.D. in Microbiology, vast experience in emerging infectious diseases, molecular biology, cancer, cell biology, and microbiology research, with a proven track record in publications, and committed to advancing scientific knowledge and contributing to innovative solutions.

• American Society of Microbiologists (ASM) 1996 – present
• Society of Biochemists, Indian Institute of Science, Bengaluru, India 1998 - present
• Microbiology Society of Singapore, NUS, Singapore 2013 – present

• Oct 2023 Spatiotemporal regulation of capsid by Ubiquitination and phosphorylation events during dengue virus infection. Duke University Joint Symposium, DukeNUS.
• Oct 2018 The concerted action of Small RabGTPase and exocyst vesicular trafficking mediates bacterial Expulsion from bladder epithelium. DUNES Symposium.
• Oct 2018 Requirement of a Distinct Inflammasome-microtubule Complex for Mast Cell Exocytosis of Granules. DUNES Symposium.
• Oct 2017 Collaboration between Distinct Rab Small GTPase Trafficking Circuits Mediates Bacterial Clearance from the Bladder Epithelium. DUNES Symposium.
• Sept 2013 Essential role of NOD-RIP2 axis in innate immunity against Acinetobacter baumannii infection. DUKE NUS DUNES Symposium.
• Sept 2013 ZNRF4 negatively regulates NOD2-mediated NF-kB signalling. DUNES Symposium. Aug 2012 NOD2 regulators in infection control, inflammation, and disease. SingHealth Duke-NUS Scientific Congress.
• Nov 2010 Annexin-1 interacts with NEMO and RIP1 to constitutively activate NF-kB signaling: Implication in breast cancer metastasis. Frontiers in Cancer Science. Cancer Science Institute. NUS
• April 2005 Mitochondrial DNA ligases of Trypanosoma brucei. Kinetoplastid Molecular Cell Biology Meeting. Marine Institute, Woods Hole, MA.
• Aug 2003 Mg2+ binding facilitates base flipping by EcoP15I DNA methyltransferase. FAOMB Conference.
• Nov 2002 Role of Magnesium in the methylation reaction catalyzed by type III restriction enzymes. Society of Biological Chemists (India), 71st Annual Meeting. Society of Biochemists.
• Dec 2001 SAM acts as an allosteric effector in the cleavage reaction catalyzed by Type III restriction enzymes. Society of Biological Chemists (India), 70th Annual Meeting. Society of Biological Chemists.
• March 2001 Molecular mechanisms that affect expression and stability of type III restriction enzymes. National Symposium on stability and stabilization of biomolecules.. National Symposium on stability and stabilization of biomolecules. Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh.
• Sept 2000 ISCOMs-Protein preparation of Immunoreactive C-terminal half of VP1 of FMDV genes elicited high-titer antibodies: Specific and cheap diagnostics. International symposium on recent advances in molecular biology, allergy, and immunology. Maharaja Sayajiro University of Baroda, Gujarat, India.
• Dec 2000 Molecular Probes for Foot-and-mouth disease virus detection and typing. Society of Biological Chemists (India), 69th Annual Meeting. Society of Biological Chemists.
• Dec 2000 SAM is required for DNA cleavage by type III restriction-modification enzymes. International Union of Biochemistry and Molecular Biology. Indian Institute of Science, Bangalore, India.
• May 1999 Sandwich nucleic acid hybridization assay as a penside test for serotyping of foot-and- mouth disease. Fifteenth annual Clinical virology symposium and Animal Meeting, Pan American Society for Clinical Virology. University of South Florida, College of Medicine, Tampa, Florida.
• Nov 1998 Activation of Foot and Mouth Disease (FMD) virus-associated endonuclease: A novel method of inactivation in FMD vaccine production.. 5th International Veterinary Immunology Symposium. University of Ludhiana, India.
• Sept 1998 Two hundred years of vaccination- A view. All India Veterinary Conference. Madras University, India.
• Feb 1998 Truncated C-terminal 16kD VP1 of Foot-Mouth-Disease virus produced in heterologous host as the type-specific diagnostic reagent. Second Pan Commonwealth Veterinary Conference on Animal health & production in rural areas, Bangalore, India. Indian Veterinary Research Institute, Bangalore, India
• Sept 1997 Purified E.coli expressed protein of FMDV as a diagnostic reagent. XIIth Annual Convention of the Indian Virological Society and National Symposium on resurgence of vector-borne viral diseases. Veterinary Institute, Anand, Gujarat, India.

• 2021 – 2023 PI: Overcoming antibiotic resistance in wound infections. IDRI- SCF/004/2021 SGH Research Grant (score-wise and review stood first) $ 50,000.00
• 2021 - 2022 Co-PI on Professor Andrea Kwa SCF/2021 Grant, $ 50,000.00
• 2001 – 2003 Postdoctoral Fellowship, Department of Biotechnology, New Delhi, Grant INR Rs 10 Lakhs

• (Oct 2025, h-factor: 29, Citation: 2568; https://scholar.google.com/citations?user=gonsRAsAAAAJ&hl=en)
• Andrea Mencarelli#, Bist Pradeep#, HW Choi, HJ Khameneh, Alessandra Mortellaro, Soman N. Abraham. Mobilization of Inflammasome Components for Anaphylactic Degranulation by Mast Cells. Nature Immunology. 2024.pr;25(4):693-702. #equal authorship. Media release link: https://www.duke-nus.edu.sg/newshub/media-releases/breakthrough-discovery-identifies-first-step-in-allergic-reactions.
• Maharah Binte Abdul Mahid, Bist Pradeep, Kristmundur Sigmundsson, Muhammad Danial Bin Mohd Mazlan, Satoru Watanabe, Milly M Choy, Subhash G Vasudevan, Kitti Wing Ki Chan. An Improved Focus-Forming Assay for Determination of the Dengue Virus Titer. BioProtocol. 2024. Oct 20;14(20):e5084.
• CX Cheng, MJA Tan, KWK Chan, MMJ Choy, N Roman, DDR Arnold, AM Bifani, SYZ Kong, Bist Pradeep, BK Nath, CMD Swarbrick, JK Forwood, Subhash G Vasudevan. Serotype-Specific Regulation of Dengue Virus NS5 Protein Subcellular Localization. ACS Infect Dis. 2024 Jun 14;10(6):2047-2062.
• Byron W Hayes, Hae Woong Choi, APS Rathore, Chunjing Bao, Jianling Shi, Yul Huh, Michael W Kim, Andrea Mencarelli, Bist Pradeep, Lai Guan Ng, Changming Shi, Joo Hwan Nho, Aram Kim, Hana Yoon, Donghoon Lim, Johanna L Hannan, J Todd Purves, Francis M Hughes Jr, Ru-Rong Ji, Soman N Abraham. Recurrent bacterial infections drive persistent bladder dysfunction and pain via sensory nerve sprouting and chronic mast cell activation. Science Immunology. 2024 Mar;9(93).
• Vasiliki Zogali, Dimitrios Kiousis, Stefania Vouytra, Georgia Kalyva, Maharah Binte Abdul Mahid, Bist Pradeep, Kitti Wing Ki Chan, Subhash G Vasudevan, Gerasimos Rassias.Carbozole to indolazepine scaffold morphing leads to potent cell-active dengue antivirals. Eur J Med Chem. 2024 Mar 15;268:116213.
• Maruo, Takuya, Sugiyama, Yukina; Kawarai, Shinpei, Anzai, Shinichi, Bist Pradeep, N, Soman Abraham. Cutaneous anaphylactoid reaction to polyoxyethylene hydrogenated castor oil in dogs. Veterinary Dermatology. 2024 Jun;35(3):263-272.
• Mencarelli A, Gunawan M, Yong KSM, Bist Pradeep, Tan WWS, Tan SY, Liu M, Huang EK, Fan Y, Chan JKY, Choi HW, Abraham SN, Chen Q. A humanized mouse model to study mast cells mediated cutaneous adverse drug reactions. Leukoc Biol. 2020 May;107(5):797-807.
• Arifuzzaman M, Mobley VR, Choi HW, Bist Pradeep, Salinas CA, Brown ZD, Chen SL, Staats HF, Abraham SN. MRGRP-mediated activation of local mast cells clears cutaneous bacterial infection and protects against reinfection. Sci Advances. 2019 Jan 2;5(1).
• Miao Y#, Bist Pradeep#, Wu J, Zhao Q, Li QJ, Wan Y, Abraham SN. Collaboration between Distinct Rab Small GTPase trafficking circuits mediates bacterial clearance from the bladder epithelium. Cell Host Microbe. 2017 Sep 13;22(3):330-342. #equal authorship.
• Bist Pradeep#, Cheong WS#, Ng A, Dikshit N, Kim BH, Pulloor NK, Khameneh HJ, Hedl M, Shenoy AR, Balamalurildhar V, Malik NBA, Hong M, Neutzner A, Chin KC, Kobayashi KS, Bertoletti A, Mortellaro A, Abraham C, MacMicking JD, Xavier RJ, Sukumaran B.E3 Ubiquitin ligase ZNRF4 negatively regulates NOD2 signalling and induces tolerance to MDP. Nat Commun. 2017 Aug 18;8:16141. #equal authorship.
• Sun B, Sundström KB, Chew JJ, Bist Pradeep, Gan ES, Tan HC, Goh KC, Chawla T, Tang CK, Ooi EE. Dengue virus activates cGAS through the release of mitochondrial DNA. Sci Rep. 2017 Jun 15;7(1):3594.
• Bist Pradeep, Kim SS, Pulloor NK, McCaffrey K, Nair SK, Liu Y, Lin R, Krishnan MN. ArfGAP Domain-Containing Protein 2 (ADAP2) Integrates Upstream and Downstream Modules of RIG-I Signaling and Facilitates Type I Interferon Production. Mol Cell Biol. 2017 Mar 1;37(6).
• Ningegowda R, Shivananjun NS, Rajendran P, Basappa, Rangappa KS, Chinnathambi A, Li F, Achar RR, Shanmugam MK, Bist Pradeep, Alharbi SA, Lim LHK, Sethi G, Priya BS.A novel 4,6-disubstituted-1,2,4-triazolo-1,3,4-thiadiazole derivative inhibits tumour cell invasion and potentiates the apoptotic effect of TNFα by abrogating NF-κB activation cascade. Apoptosis. 2017 Jan;22(1):145-157.
• Nair S, Bist Pradeep, Dikshit N, Krishnan MN. Global functional profiling of human ubiquitome identifies E3 ubiquitin ligase DCST1 as a novel negative regulator of Type-I interferon signaling. Sci Rep. 2016 Oct 26;6:36179.
• Arora S, Lim W, Bist Pradeep, Peramalsamy R, Lukman HM, Li F, Welker LB, Yan B, Sethi G, Tambyah PA, Fairhurst AM, Alonso S, Lim LH. Influenza A virus enhances its propagation through the modulation of Annexin-A1 dependent endosomal trafficking and apoptosis. Cell Death Differ. 2016 Jul;23(7):1243-56.
• Dikshit N, Bist Pradeep, Fenlon SN, Pulloor NK, Chua CE, Scidmore MA, Carlyon JA, Tang BL, Chen SL, Sukumaran B. Intracellular Uropathogenic E. coli Exploits Host Rab35 for Iron Acquisition and Survival within Urinary Bladder Cells. PLoS Pathog. 2015 Aug 6;11(8).
• Bist Pradeep, Phua QH, Shu S, Yi Y, Anbalagan D, Lee LH, Sethi G, Annexin-A1 controls an ERK-RhoA-NFκB activation loop in breast cancer cells. BBRC. 2015 May 22;461(1):47-53.
• Lee KG, Kim SS, Kui L, Voon DC, Mauduit M, Bist Pradeep, Bi X, Pereira NA, Liu C, Sukumaran B, Rénia L, Ito Y, Lam KP. Bruton's tyrosine kinase phosphorylates DDX41 and activates its binding of dsDNA and STING to initiate type 1 interferon response. Cell Rep. 2015 Feb 24;10(7):1055-65.
• Anbalagan D, Yap G, Yuan Y, Pandey VK, Lau WH, Arora S, Bist Pradeep, Wong JS, Sethi G, Nissom PM, Lobie PE, Lim LH. Annexin-A1 regulates microRNA-26b
• And microRNA-562 to directly target NF-κB and angiogenesis in breast cancer cells. PLoS One. 2014 Dec 23;9(12):e114507.
• Pulloor NK, Nair S, McCaffrey K, Kostic AD, Bist Pradeep, Weaver JD, Riley AM, Tyagi R, Uchil PD, York JD, Snyder SH, García-Sastre A, Potter BV, Lin R, Shears SB, Xavier RJ, Krishnan MN. Human genome-wide RNAi screen identifies an essential role for inositol pyrophosphates in Type-I interferon response. PLoS Pathog. 2014 Feb 27;10(2):e1003981.
• Bist Pradeep, Dikshit N, Koh TH, Mortellaro A, Tan TT, Sukumaran B. The Nod1, Nod2, and Rip2 axis contributes to host immune defense against intracellular Acinetobacter baumannii infection. Infect Immun. 2014 Mar;82(3):1112-22.
• Bist Pradeep, Shu S, Lee H, Arora S, Nair S, Lim JY, Dayalan J, Gasser S, Biswas SK, Fairhurst AM, Lim LH.J. Annexin-A1 regulates TLR-mediated IFN-β production through an interaction with TANK-binding kinase 1. J Immunol. 2013 Oct 15;191(8):4375-82.
• Kurup A, Issac A, Loh JP, Lee TB, Chua R, Bist Pradeep, Chao CC, Lewis M, Gubler DJ, Ching WM, Ooi EE, Sukumaran B Scrub typhus with sepsis and acute respiratory distress syndrome. J Clin Microbiol. 2013 Aug;51(8):2787-90.
• Manu KA, Shanmugam MK, Ong TH, Subramanian A, Siveen KS, Perumal E, Samy RP, Bist Pradeep, Lim LH, Kumar AP, Hui KM, Sethi G. Emodin suppresses migration and invasion through the modulation of CXCR4 expression in an orthotopic model of human hepatocellular carcinoma. PLoS One. 2013;8(3):e57015.
• Ng FS, Wong KY, Guan SP, Mustafa FB, Kajiji TS, Bist Pradeep, Biswas SK, Wong WS, Lim LH. Annexin-1-deficient mice exhibit spontaneous airway hyperresponsiveness and exacerbated allergen-specific antibody responses in a mouse model of asthma. Clin Exp Allergy. 2011 Dec;41(12):1793-803.
• Manu KA, Shanmugam MK, Rajendran P, Li F, Ramachandran L, Hay HS, Kannaiyan R, Swamy SN, Vali S, Kapoor S, Ramesh B, Bist Pradeep, Koay ES, Lim LH, Ahn KS, Kumar AP, Sethi G. Plumbagin inhibits invasion and migration of breast and gastric cancer cells by downregulating the expression of chemokine receptor CXCR4. Mol Cancer. 2011 Sep 1;10:107.
• Shanmugam MK, Manu KA, Ong TH, Ramachandran L, Surana R, Bist Pradeep, Lim LH, Kumar AP, Hui KM, Sethi G. Inhibition of CXCR4/CXCL12 signaling axis by ursolic acid leads to suppression of metastasis in transgenic adenocarcinoma of mouse prostate model. Int J Cancer. 2011 Oct 1;129(7):1552-63.
• Bist Pradeep, Leow SC, Phua QH, Shu S, Zhuang Q, Loh WT, Nguyen TH, Zhou JB, Hooi SC, Lim LH. Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. Oncogene. 2011 Jul 14;30(28):3174-85.
• Chua AW, Hay HS, Rajendran P, Shanmugam MK, Li F, Bist Pradeep, Koay ES, Lim LH, Kumar AP, Sethi G. Butein downregulates chemokine receptor CXCR4 expression and function through suppression of NF-κB activation in breast and pancreatic tumor cells. Biochem Pharmacol. 2010 Nov 15;80(10):1553-62.
• Singh K, Sinha S, Malonia SK, Bist Pradeep, Tergaonkar V, Chattopadhyay S. Tumor suppressor SMAR1 represses IkappaBalpha expression and inhibits p65 transactivation through matrix attachment regions. J Biol Chem. 2009 Jan 9;284(2):1267-78. doi: 10.1074/jbc.M801088200. Epub 2008 Nov 3.
• Dey A, Wong ET, Bist Pradeep, Tergaonkar V, Lane DP. Nutlin-3 inhibits the NFkappab pathway in a p53-dependent manner: implications in lung cancer therapy. Cell Cycle. 2007 Sep 1;6(17):2178-85.
• Biswas SK, Bist Pradeep, Dhillon MK, Kajiji T, Del Fresno C, Yamamoto M, Lopez-Collazo E, Akira S, Tergaonkar V. Role for MyD88-independent, TRIF pathway in lipid A/TLR4-induced endotoxin tolerance. J Immunol. 2007 Sep 15;179(6):4083-92.
• Bist Pradeep, Madhusoodanan UK, Rao DN. A mutation in the Mod subunit of EcoP15I restriction enzyme converts the DNA methyltransferase to a site-specific endonuclease. J Biol Chem. 2007 Feb 9;282(6):3520-30.
• Ghosh S, Tergaonkar V, Rothlin CV, Correa RG, Bottero V, Bist Pradeep, Verma IM, Hunter T. Essential role of tuberous sclerosis genes TSC1 and TSC2 in NF-kappaB activation and cell survival. Cancer Cell. 2006 Sep;10(3):215-26.
• Bist Pradeep, Rao DN. Identification and mutational analysis of Mg2+ binding site in EcoP15I DNA methyltransferase: involvement in target base eversion. J Biol Chem. 2003 Oct 24;278(43):41837-48.
• Patil PK, Suryanarayana V, Bist Pradeep, Bayry J, Natarajan C.Integrity of GH-loop of foot-and-mouth disease virus during virus inactivation: detection by epitope specific antibodies. Vaccine. 2002 Jan 15;20(7-8):1163-8.
• Bist Pradeep, Sistla S, Krishnamurthy V, Acharya A, Chandrakala B, Rao DN.S-adenosyl-L-methionine is required for DNA cleavage by type III restriction enzymes. J Mol Biol. 2001 Jun 29;310(1):93-109.
• Suryanarayana VV, Viswanathan S, Ratish G, Bist Pradeep, Prabhudas K, Gajendragad MR, Natarajan C. E. coli expressed proteins as diagnostic reagents for typing of foot-and-mouth disease virus. Arch Virol. 1999;144(9):1701-12.
• Bayry J, Prabhudas K, Bist Pradeep, Reddy GR, Suryanarayana VV. Immunoaffinity purification of foot and mouth disease virus type-specific antibodies using recombinant protein adsorbed to polystyrene wells. J Virol Methods. 1999 Aug;81(1-2):21-30.
• Suryanarayana V, Madanmohan B, Bist Pradeep, Natarajan C, Tratschin JD. Serotyping of foot-and-mouth disease virus by antigen capture reverse transcriptase/polymerase chain reaction. J Virol Methods. 1999 Jun;80(1):45-52.
• Suryanarayana V, Bist Pradeep, GR Reddy, LD Misra. Serotyping of foot and mouth disease virus from aerosols in the infected area. 1999. Current Science. Vol 76, No. 10: 1388-1391. https://www.currentscience.ac.in/Volumes/76/10/1388.pdf
• Olivia Goethals, Doortje Borenberghs, Kitti Wing Ki Chan, Sven Van Brandt, Steffen Jaensch, Milly Choy, Peggy Geluykens, Asmae El Bouayadi, Dax Lauwers, Pradeep Bist, Bart Stoops, Jeroen Van de Ven, Peter Vermeulen, Sarah Megens, Danielle Peeters, Suzanne Kaptein, Johan Neyts, Subhash Vasudevan, Anil Koul. Identification of a broad-spectrum flavivirus inhibitor targeting NS2A, a previously unidentified target. Nature Commun. 2025