Summary
Overview
Work History
Education
Skills
Workshops And Trainings
Conferences And Presentations
Languages
Scholarships And Fellowships
Publications
Book Chapters
Timeline
Generic

Prasoon Madhukar

Varanasi

Summary

PhD graduate specializing in nanoparticle-based drug delivery, with expertise in formulation design, molecular diagnostics, cell and animal studies, and immunology. Experienced in developing innovative research strategies, managing cross-functional projects, and producing high-quality scientific documentation. Driven to advance biotechnology and therapeutic development through cutting-edge research. Seeking a role in biotech or pharma to apply expertise in translational research and drug development

Overview

9
9
years of professional experience

Work History

Instructor

AcadSort
10.2021 - Current
  • After my lab timing, I train students preparing for esteemed assessments, such as the Eton (King's) Scholarship examination, AP Biology exam, Cambridge IGCSE Biology exam, A-Level Biology, and MCAT.

PhD Research

Institute of Medical Sciences, Banaras Hindu University
Varanasi
01.2020 - 03.2025
  • Design and Optimization of a Hybrid Nanoparticle-Amphotericin B Formulation Developed a novel Amphotericin B formulation using graphene oxide-CNT hybrid nanoparticles to enhance drug efficacy, prevent aggregation, and improve stability
  • Graphene Quantum Dots (GQDs) for Targeted Drug Delivery and Bioimaging Engineered GQDs as a dual-function cargo for drug delivery and bioimaging, optimizing controlled release, cellular uptake, and therapeutic efficiency
  • Nanoparticle Uptake, Cytotoxicity, and Immune Response Analysis Investigated the intracellular fate of drug-loaded nanoparticles in macrophages, assessing cytotoxicity, degradation, and immune modulation in vitro and in vivo
  • Mannosylated GQD-Amphotericin B for Leishmaniasis Treatment Evaluated the efficacy, biocompatibility, biodistribution, and immune response of mannose-functionalized GQD-Amphotericin B nanoparticles for targeted Leishmaniasis therapy

Visiting Doctoral Fellow

Institute of Nano Science and Technology
Mohali
01.2023 - 05.2023
  • Synthesized mannose-functionalized Graphene Quantum Dots (GQDs) conjugated with Amphotericin B and evaluated their in vitro safety and antileishmanial efficacy
  • Designed and optimized nanocarrier systems, including ultradeformable particles (UDPs), solid lipid nanoparticles (SLNs), and nanofibers
  • Gained hands-on experience with nanoparticle characterization techniques such as DLS, XRD, AFM, FTIR, and SEM

Researcher

DetectoGen Inc.
MA
02.2021 - 11.2022
  • Concurrent with my PhD research, contributed to the clinical validation of a monoclonal antibody-based multiplexed capture ELISA for visceral leishmaniasis using urine samples
  • Managed sample collection, performed ELISA, conducted data analysis, and prepared reports while ensuring compliance with regulatory standards for clinical research

Assistant Lecturer

Aakash Institute
07.2017 - 02.2019
  • Prepared students for the Zoology portion of the National Eligibility-cum-Entrance Test (NEET) for admission into undergraduate medical courses across India

MSc Dissertation

Institute of Science, BHU
01.2017 - 05.2017
  • Developed and validated a Streptozotocin-induced mouse model for Alzheimer's disease, focusing on biochemical and molecular alterations
  • Gained hands-on experience in animal handling, stereotaxy, intracerebroventricular (ICV) administration, transcardial perfusion, histology, light and fluorescence microscopy, histochemistry, Western blotting, and biochemical assays

Summer Intern

Indian Association for the Cultivation of Science (IACS)
Kolkata
05.2016 - 06.2016
  • Monitored Tissue Factor-GFP activity in live mammalian cells, contributing to research on the coagulation cascade
  • Utilized molecular biology techniques, including PCR, gel electrophoresis, bacterial culture, transformation, plasmid isolation, restriction digestion, and hybridization

Education

Doctor of Philosophy (PhD) - Dept. of Medicine

Institute of Medical Sciences, Banaras Hindu University
India
03.2025

Master of Science (MSc) - Zoology

Institute of Science, Banaras Hindu University
India
06.2017

Bachelor of Science (BSc) - Zoology (Honors), Botany, Chemistry

Institute of Science, Banaras Hindu University
India
07.2015

Skills

  • Nanoparticle synthesis: thermal exfoliation, spray-assisted CVD, hydrothermal and solvothermal synthesis, green synthesis, thin film hydration, microemulsion method
  • Characterization techniques: DLS, XRD, FTIR, UV-Visible spectroscopy, and fluorescence and electron microscopy
  • Diagnostic Techniques: ELISA, Direct Agglutination Test (DAT), PCR, RT-PCR, FACS
  • Cell culture: cytotoxicity assay, antileishmanial assay, uptake study
  • Animal studies: antileishmanial study, toxicity assessment, PK studies, histology, tissue biodistribution study
  • HPLC & Lyophilizer (operator level efficiency)
  • Flow cytometry data analysis using FCAP Array software
  • Statistical tools: GraphPad Prism 80, Origin 2018 and Excel
  • Image analysis tools: Fiji (ImageJ), ZEN

Workshops And Trainings

  • Nanomaterials Synthesis & Characterization, SATHI, BHU, 06/23
  • Sophisticated R&D Equipment Training, SATHI BHU, 12/22
  • RT-PCR & Next-Generation Sequencing Workshop, SERB, BHU, 11/23
  • Diploma in Statistics & Computing, BHU, 01/22 - 10/22
  • CeNSE DBT Nano-biotechnology Alliance (C-DNA) Familiarization workshop, IISc, Bangalore, 09/20
  • International Webinar on Materials Synthesis and Characterization, Dr RML Awadh University and Florida Polytechnic University, 2020

Conferences And Presentations

  • Best Poster Award, Advanced Materials for Better Tomorrow (AMBT), BHU, 10/23
  • Best Poster Award, Recent Trends in Animal Sciences (RTAS), BHU, 2022
  • Travel Grant Recipient, ESCMID Global 2024, Barcelona, Spain, 04/24
  • Poster Presentation, International conference on Trends in Emerging Nanoscience, Institute of Nanoscience and Technology, Mohali, India, 11/23
  • Outreach Grant Recipient, ECCMID, Copenhagen, Denmark, 04/23
  • E-Poster Presentation, ASTMH Annual Meeting, Seattle, USA, 2022
  • International Travel Support, SERB, Govt. of India, ICOPA, Copenhagen, Denmark, 08/22
  • Poster Presentation, CIDSCON, Kolkata, India, 07/22

Languages

  • English, Fluent
  • Hindi, Fluent
  • Bengali, Working Proficiency
  • Spanish, Elementary proficiency

Scholarships And Fellowships

  • CSIR-UGC: Junior Research Fellowship (JRF) & NET Eligibility, 2019
  • ICMR: Junior Research Fellowship (JRF), 2019
  • Graduate Aptitude Test in Engineering (GATE), Life Sciences, 2020

Publications

  • Amine-Functionalized Graphene Quantum Dots Conjugated with Amphotericin B: Synthesis, Characterization, and In-Vitro Evaluation for Visceral Leishmaniasis Treatment., Madhukar P.; Kesarwani R.; Panday S.; Singh V.K.; Gedda M.R.; Shaz M.A.; Kumar R.; Sundar S., ACS Omega, Under Review, 2025
  • Anti-leishmanial therapies: Overcoming current challenges with emerging therapies., Sundar S.; Madhukar P.; Kumar R., Expert Review of Anti-infective Therapy, 2024
  • Dual Drug Delivery for Augmenting Bacterial Wound Complications via Tailored Ultradeformable Carriers., Arora, K.; Dhruw, B.; Pm, S.; Madhukar P.; Sundar, S.; Mudavath, S. L., Bioconjug Chem, 2024
  • Evaluation of Blood Based Quantitative PCR as a Molecular Diagnostic Tool for Post Kala-Azar Dermal Leishmaniasis (PKDL)., Kumar, A.; Singh, V. K.; Madhukar P.; Tiwari, R.; Roy, R.; Rajneesh; Mehrotra, S.; Sundar, S.; Kumar, R., Mol Biol Rep, 2024
  • Nanotechnology-Based Strategies in Parasitic Disease Management: From Prevention to Diagnosis and Treatment., Tiwari, R.; Gupta, R. P.; Singh, V. K.; Kumar, A.; Rajneesh, N.; Madhukar P.; Sundar, S.; Gautam, V.; Kumar, R., ACS Omega, 2023
  • Post Kala-Azar Dermal Leishmaniasis in the Indian Sub-Continent: Challenges and Strategies for Elimination., Kumar, A.; Singh, V. K.; Tiwari, R.; Madhukar P.; Rajneesh; Kumar, S.; Gautam, V.; Engwerda, C.; Sundar, S.; Kumar, R., Front Immunol, 2023
  • Ethyl Acetate Extract of Colletotrichum Gloeosporioides Promotes Cytotoxicity and Apoptosis in Human Breast Cancer Cells., Rai, N.; Gupta, P.; Verma, A.; Tiwari, R. K.; Madhukar P.; Kamble, S. C.; Kumar, A.; Kumar, R.; Singh, S. K.; Gautam, V., ACS Omega, 2022
  • Nanodiagnostics in Leishmaniasis: A New Frontiers for Early Elimination., Gedda, M. R.; Madhukar P.; Shukla, A.; Mudavath, S. L.; Srivastava, O. N.; Singh, O. P.; Sundar, S., Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, 2021
  • Post Kala-Azar Dermal Leishmaniasis: A Threat to Elimination Program., Gedda, M. R.; Madhukar P.; Singh, B.; Kumar, D.; Singh, A. K.; Upadhyay, S.; Singh, O. P.; Sundar, S., PLoS Neglected Tropical Diseases, 2020
  • Evaluation of Safety and Antileishmanial Efficacy of Amine Functionalized Carbon-Based Composite Nanoparticle Appended With Amphotericin B: An in Vitro and Preclinical Study., Gedda, M. R.; Madhukar P. (Equal contribution); Vishwakarma, A. K.; Verma, V.; Kushwaha, A. K.; Yadagiri, G.; Mudavath, S. L.; Singh, O. P.; Srivastava, O. N.; Sundar, S., Front Chem, 2020
  • Epigenetic Aspects of Engineered Nanomaterials: Is the Collateral Damage Inevitable?, Gedda, M. R.; Babele, P. K.; Zahra, K.; Madhukar P (Equal Contribution)., Frontiers in Bioengineering and Biotechnology, 2019
  • Blood Biomarkers for Ischemic Stroke Subtype Differentiation: A Systematic Review., Pandey, S. P.; Madhukar P.; Dev, P.; Joshi, D.; Mishra, V. N.; Chaurasia, R. N.; Pathak, A., Cardiovascular & Hematological Disorders-Drug Targets, 2018

Book Chapters

  • Synthesis of Porous Silicon-based Nanoparticles., Madhukar, P.; Singh S.; Sundar S., Elsevier, 2025
  • Characterization techniques for mesoporous silicon nanoparticles., Madhukar, P.; Murya P.; Kashyap S.; Sundar S., Elsevier, 2025
  • Nanomedicine in Cardiovascular Diseases: Diagnostic and Therapeutic Innovations for Better Health., Madhukar, P.; Singh K.V.; Chauhan S.B.; Tiwari R.; Kumar A.; Rajneesh; Kumar R., Springer Nature, 2024
  • MicroRNAs-Mediated Regulation of Immune Responses in Parasitic Infection., Roy, R.; Madhukar, P.; Singh, V. K.; Tiwari, R.; Kumar, A.; Rajneesh; Rai, M.; Gautam, V.; Sundar, S.; Kumar, R., MicroRNA in Human Infectious Diseases, 2024
  • Post-Stroke Treatment Strategies, Management, and Rehabilitation: Where We Stand?, Gedda, M. R.; Pandey, S. P.; Zahra, K.; Madhukar, P.; Pathak, A., Advancement in the Pathophysiology of Cerebral Stroke, 2019

Timeline

Visiting Doctoral Fellow

Institute of Nano Science and Technology
01.2023 - 05.2023

Instructor

AcadSort
10.2021 - Current

Researcher

DetectoGen Inc.
02.2021 - 11.2022

PhD Research

Institute of Medical Sciences, Banaras Hindu University
01.2020 - 03.2025

Assistant Lecturer

Aakash Institute
07.2017 - 02.2019

MSc Dissertation

Institute of Science, BHU
01.2017 - 05.2017

Summer Intern

Indian Association for the Cultivation of Science (IACS)
05.2016 - 06.2016

Doctor of Philosophy (PhD) - Dept. of Medicine

Institute of Medical Sciences, Banaras Hindu University

Master of Science (MSc) - Zoology

Institute of Science, Banaras Hindu University

Bachelor of Science (BSc) - Zoology (Honors), Botany, Chemistry

Institute of Science, Banaras Hindu University
Prasoon Madhukar