PhD graduate specializing in nanoparticle-based drug delivery, with expertise in formulation design, molecular diagnostics, cell and animal studies, and immunology. Experienced in developing innovative research strategies, managing cross-functional projects, and producing high-quality scientific documentation. Driven to advance biotechnology and therapeutic development through cutting-edge research. Seeking a role in biotech or pharma to apply expertise in translational research and drug development
Overview
9
9
years of professional experience
Work History
Instructor
AcadSort
10.2021 - Current
After my lab timing, I train students preparing for esteemed assessments, such as the Eton (King's) Scholarship examination, AP Biology exam, Cambridge IGCSE Biology exam, A-Level Biology, and MCAT.
PhD Research
Institute of Medical Sciences, Banaras Hindu University
Varanasi
01.2020 - 03.2025
Design and Optimization of a Hybrid Nanoparticle-Amphotericin B Formulation Developed a novel Amphotericin B formulation using graphene oxide-CNT hybrid nanoparticles to enhance drug efficacy, prevent aggregation, and improve stability
Graphene Quantum Dots (GQDs) for Targeted Drug Delivery and Bioimaging Engineered GQDs as a dual-function cargo for drug delivery and bioimaging, optimizing controlled release, cellular uptake, and therapeutic efficiency
Nanoparticle Uptake, Cytotoxicity, and Immune Response Analysis Investigated the intracellular fate of drug-loaded nanoparticles in macrophages, assessing cytotoxicity, degradation, and immune modulation in vitro and in vivo
Mannosylated GQD-Amphotericin B for Leishmaniasis Treatment Evaluated the efficacy, biocompatibility, biodistribution, and immune response of mannose-functionalized GQD-Amphotericin B nanoparticles for targeted Leishmaniasis therapy
Visiting Doctoral Fellow
Institute of Nano Science and Technology
Mohali
01.2023 - 05.2023
Synthesized mannose-functionalized Graphene Quantum Dots (GQDs) conjugated with Amphotericin B and evaluated their in vitro safety and antileishmanial efficacy
Designed and optimized nanocarrier systems, including ultradeformable particles (UDPs), solid lipid nanoparticles (SLNs), and nanofibers
Gained hands-on experience with nanoparticle characterization techniques such as DLS, XRD, AFM, FTIR, and SEM
Researcher
DetectoGen Inc.
MA
02.2021 - 11.2022
Concurrent with my PhD research, contributed to the clinical validation of a monoclonal antibody-based multiplexed capture ELISA for visceral leishmaniasis using urine samples
Managed sample collection, performed ELISA, conducted data analysis, and prepared reports while ensuring compliance with regulatory standards for clinical research
Assistant Lecturer
Aakash Institute
07.2017 - 02.2019
Prepared students for the Zoology portion of the National Eligibility-cum-Entrance Test (NEET) for admission into undergraduate medical courses across India
MSc Dissertation
Institute of Science, BHU
01.2017 - 05.2017
Developed and validated a Streptozotocin-induced mouse model for Alzheimer's disease, focusing on biochemical and molecular alterations
Gained hands-on experience in animal handling, stereotaxy, intracerebroventricular (ICV) administration, transcardial perfusion, histology, light and fluorescence microscopy, histochemistry, Western blotting, and biochemical assays
Summer Intern
Indian Association for the Cultivation of Science (IACS)
Kolkata
05.2016 - 06.2016
Monitored Tissue Factor-GFP activity in live mammalian cells, contributing to research on the coagulation cascade
Utilized molecular biology techniques, including PCR, gel electrophoresis, bacterial culture, transformation, plasmid isolation, restriction digestion, and hybridization
Education
Doctor of Philosophy (PhD) - Dept. of Medicine
Institute of Medical Sciences, Banaras Hindu University
India
03.2025
Master of Science (MSc) - Zoology
Institute of Science, Banaras Hindu University
India
06.2017
Bachelor of Science (BSc) - Zoology (Honors), Botany, Chemistry
Institute of Science, Banaras Hindu University
India
07.2015
Skills
Nanoparticle synthesis: thermal exfoliation, spray-assisted CVD, hydrothermal and solvothermal synthesis, green synthesis, thin film hydration, microemulsion method
Characterization techniques: DLS, XRD, FTIR, UV-Visible spectroscopy, and fluorescence and electron microscopy
Diagnostic Techniques: ELISA, Direct Agglutination Test (DAT), PCR, RT-PCR, FACS
Cell culture: cytotoxicity assay, antileishmanial assay, uptake study
International Webinar on Materials Synthesis and Characterization, Dr RML Awadh University and Florida Polytechnic University, 2020
Conferences And Presentations
Best Poster Award, Advanced Materials for Better Tomorrow (AMBT), BHU, 10/23
Best Poster Award, Recent Trends in Animal Sciences (RTAS), BHU, 2022
Travel Grant Recipient, ESCMID Global 2024, Barcelona, Spain, 04/24
Poster Presentation, International conference on Trends in Emerging Nanoscience, Institute of Nanoscience and Technology, Mohali, India, 11/23
Outreach Grant Recipient, ECCMID, Copenhagen, Denmark, 04/23
E-Poster Presentation, ASTMH Annual Meeting, Seattle, USA, 2022
International Travel Support, SERB, Govt. of India, ICOPA, Copenhagen, Denmark, 08/22
Poster Presentation, CIDSCON, Kolkata, India, 07/22
Languages
English, Fluent
Hindi, Fluent
Bengali, Working Proficiency
Spanish, Elementary proficiency
Scholarships And Fellowships
CSIR-UGC: Junior Research Fellowship (JRF) & NET Eligibility, 2019
ICMR: Junior Research Fellowship (JRF), 2019
Graduate Aptitude Test in Engineering (GATE), Life Sciences, 2020
Publications
Amine-Functionalized Graphene Quantum Dots Conjugated with Amphotericin B: Synthesis, Characterization, and In-Vitro Evaluation for Visceral Leishmaniasis Treatment., Madhukar P.; Kesarwani R.; Panday S.; Singh V.K.; Gedda M.R.; Shaz M.A.; Kumar R.; Sundar S., ACS Omega, Under Review, 2025
Anti-leishmanial therapies: Overcoming current challenges with emerging therapies., Sundar S.; Madhukar P.; Kumar R., Expert Review of Anti-infective Therapy, 2024
Dual Drug Delivery for Augmenting Bacterial Wound Complications via Tailored Ultradeformable Carriers., Arora, K.; Dhruw, B.; Pm, S.; Madhukar P.; Sundar, S.; Mudavath, S. L., Bioconjug Chem, 2024
Evaluation of Blood Based Quantitative PCR as a Molecular Diagnostic Tool for Post Kala-Azar Dermal Leishmaniasis (PKDL)., Kumar, A.; Singh, V. K.; Madhukar P.; Tiwari, R.; Roy, R.; Rajneesh; Mehrotra, S.; Sundar, S.; Kumar, R., Mol Biol Rep, 2024
Nanotechnology-Based Strategies in Parasitic Disease Management: From Prevention to Diagnosis and Treatment., Tiwari, R.; Gupta, R. P.; Singh, V. K.; Kumar, A.; Rajneesh, N.; Madhukar P.; Sundar, S.; Gautam, V.; Kumar, R., ACS Omega, 2023
Post Kala-Azar Dermal Leishmaniasis in the Indian Sub-Continent: Challenges and Strategies for Elimination., Kumar, A.; Singh, V. K.; Tiwari, R.; Madhukar P.; Rajneesh; Kumar, S.; Gautam, V.; Engwerda, C.; Sundar, S.; Kumar, R., Front Immunol, 2023
Ethyl Acetate Extract of Colletotrichum Gloeosporioides Promotes Cytotoxicity and Apoptosis in Human Breast Cancer Cells., Rai, N.; Gupta, P.; Verma, A.; Tiwari, R. K.; Madhukar P.; Kamble, S. C.; Kumar, A.; Kumar, R.; Singh, S. K.; Gautam, V., ACS Omega, 2022
Nanodiagnostics in Leishmaniasis: A New Frontiers for Early Elimination., Gedda, M. R.; Madhukar P.; Shukla, A.; Mudavath, S. L.; Srivastava, O. N.; Singh, O. P.; Sundar, S., Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, 2021
Post Kala-Azar Dermal Leishmaniasis: A Threat to Elimination Program., Gedda, M. R.; Madhukar P.; Singh, B.; Kumar, D.; Singh, A. K.; Upadhyay, S.; Singh, O. P.; Sundar, S., PLoS Neglected Tropical Diseases, 2020
Evaluation of Safety and Antileishmanial Efficacy of Amine Functionalized Carbon-Based Composite Nanoparticle Appended With Amphotericin B: An in Vitro and Preclinical Study., Gedda, M. R.; Madhukar P. (Equal contribution); Vishwakarma, A. K.; Verma, V.; Kushwaha, A. K.; Yadagiri, G.; Mudavath, S. L.; Singh, O. P.; Srivastava, O. N.; Sundar, S., Front Chem, 2020
Epigenetic Aspects of Engineered Nanomaterials: Is the Collateral Damage Inevitable?, Gedda, M. R.; Babele, P. K.; Zahra, K.; Madhukar P (Equal Contribution)., Frontiers in Bioengineering and Biotechnology, 2019
Blood Biomarkers for Ischemic Stroke Subtype Differentiation: A Systematic Review., Pandey, S. P.; Madhukar P.; Dev, P.; Joshi, D.; Mishra, V. N.; Chaurasia, R. N.; Pathak, A., Cardiovascular & Hematological Disorders-Drug Targets, 2018
Nanomedicine in Cardiovascular Diseases: Diagnostic and Therapeutic Innovations for Better Health., Madhukar, P.; Singh K.V.; Chauhan S.B.; Tiwari R.; Kumar A.; Rajneesh; Kumar R., Springer Nature, 2024
MicroRNAs-Mediated Regulation of Immune Responses in Parasitic Infection., Roy, R.; Madhukar, P.; Singh, V. K.; Tiwari, R.; Kumar, A.; Rajneesh; Rai, M.; Gautam, V.; Sundar, S.; Kumar, R., MicroRNA in Human Infectious Diseases, 2024
Post-Stroke Treatment Strategies, Management, and Rehabilitation: Where We Stand?, Gedda, M. R.; Pandey, S. P.; Zahra, K.; Madhukar, P.; Pathak, A., Advancement in the Pathophysiology of Cerebral Stroke, 2019
Timeline
Visiting Doctoral Fellow
Institute of Nano Science and Technology
01.2023 - 05.2023
Instructor
AcadSort
10.2021 - Current
Researcher
DetectoGen Inc.
02.2021 - 11.2022
PhD Research
Institute of Medical Sciences, Banaras Hindu University
01.2020 - 03.2025
Assistant Lecturer
Aakash Institute
07.2017 - 02.2019
MSc Dissertation
Institute of Science, BHU
01.2017 - 05.2017
Summer Intern
Indian Association for the Cultivation of Science (IACS)
05.2016 - 06.2016
Doctor of Philosophy (PhD) - Dept. of Medicine
Institute of Medical Sciences, Banaras Hindu University
Master of Science (MSc) - Zoology
Institute of Science, Banaras Hindu University
Bachelor of Science (BSc) - Zoology (Honors), Botany, Chemistry