Prem Kumar Gupta, Ph.D.

Accomplished Senior Scientist with over 15 years of experience in Life Sciences research, specializing in LC-MS/MS method development and analysis of small molecules, peptides, and proteins. Experienced in leading teams and managing laboratory operations to ensure compliance with industry standards. Committed to advancing scientific knowledge through innovative research and effective communication.

• H. Hendrickson, P.K. Gupta, R. Pathak, M. Hubbard, M. Hauer-Jensen, Biopterin Metabolome- Unlocking the Mysteries of Ionizing Radiation Injury, ABRF Annual Conference, 2013, Palm Springs, CA, USA.
• Gupta, P.K., J. Brown, M. Hauer-Jensen, H.P. Hendrickson, Development and Cross-Species Validation of High-throughput UPLC-MS/MS Methodology for the Determination of Underivatized Plasma Citrulline, AAPS Annual Meeting and Symposium, 2010, New Orleans, LA, USA.
• Gupta, P.K., G. Barone, B.G. Gurley, H.P. Hendrickson, Clinical Pharmacokinetics and Metabolism of Berberine and Hydrastine Following an Oral Dose of Goldseal Supplement, AAPS Annual Meeting and Symposium, 2009, Los Angeles, CA, USA.
• Gupta, P.K., G. Barone, B.G. Burley, H.P. Hendrickson, Metabolism of Berberine and Hydrastine Following a Single Oral Dose of Goldseal Supplement, American Chemical Society Regional Meeting, 2008, Little Rock, AR, USA.
• Gupta, P.K., M. Hubbard, G. Barone, B.G. Gurley, H.P. Hendrickson, Validation of a Liquid Chromatography-Tandem Mass Spectrometric Assay for the Determination of Hydrastine and Berberine in Human Serum, AAPS Annual Meeting and Symposium, 2008, Atlanta, GA, USA.
• Gupta, P.K., P. Jewaria, S. Sopory, N. Bhalla-Sarin, Mechanism of Multiple Shoot Formation from the Cotyledonary Node of Cajanus Cajan, BioSpark-2007, School of Life Sciences, Jawaharlal Nehru University, 2007, New Delhi, India.

• AAPS Postdoctoral Travel Award to attend and present poster during 2008 annual meeting in Atlanta, GA.
• Received Council for Scientific and Industrial Research (CSIR), India, research fellowship for doctoral studies.
• Ranked 3rd in the All-India Entrance Examination for Ph.D. Life Sciences program of Jawaharlal Nehru University.
• Ranked 1st in the All-India Entrance Examination for M.Sc. Biotechnology program of Agra University.

• Yasheshwar, Gaurav, Ekkbal, R., Gupta, P., & Gyawali, R. (2024). System Pharmacological approach to investigate and validate multitargeted and therapeutic effect of furocoumarins of Apium graveolens L. for treatment of kidney disease. Advances in Pharmacological and Pharmaceutical Sciences, 2024(1), 5543561.
• P. Gupta, A. Rustagi, P.K. Gupta, Herbicide-tolerant soybean: Past, present, & future, Soybean: History, taxonomy, genetics, breeding, diseases, and biotechnology, Apple Academic Press, Florida, USA
• Sood, V., Rawat, D., Khanna, R., Sharma, S., Gupta, P. K., Alam, S., & Sarin, S. K. (2017). Study of carnitine/acylcarnitine and amino acid profile in children and adults with acute liver failure. Journal of Pediatric Gastroenterology and Nutrition, 64(6), 869-875.
• Gupta, P. K., Barone, G., Gurley, B. J., Fifer, E. K., & Hendrickson, H. P. (2015). Hydrastine pharmacokinetics and metabolism after a single oral dose of goldenseal (Hydrastis canadensis) to humans. Drug Metabolism and Disposition, 43(4), 534-552.
• Biju, P. G., Gubrij, I., Garg, S., Gupta, P. K., Hauer-Jensen, M., & Burnett, A. F. (2014). Radiation lethality potentiation in total body irradiated mice by a commonly prescribed proton pump inhibitor, pantoprazole sodium. International journal of radiation biology, 90(7), 554-559.
• Pathak, R., Pawar, S. A., Fu, Q., Gupta, P. K., Berbée, M., Garg, S., ... & Hauer-Jensen, M. (2014). Characterization of transgenic Gfrp knock-in mice: implications for tetrahydrobiopterin in modulation of normal tissue radiation responses. Antioxidants & Redox Signaling, 20(9), 1436-1446.
• Gupta, P. K., Brown, J., Biju, P. G., Thaden, J., Deutz, N. E., Kumar, S., ... & Hendrickson, H. P. (2011). Development of high-throughput HILIC-MS/MS methodology for plasma citrulline determination in multiple species. Analytical Methods, 3(8), 1759-1768.
• Krager, K. J., Patak, R., Pawar, S. A., Gupta, P., Fu, Q., Berbée, M., ... & Burns, N. A., De Novo Biosynthesis of Tetrahydrobiopterin is Critical in Protecting against Ionizing Radiation. Free Radical Biology and Medicine, 53, S182.
• Gupta, P. K., Hubbard, M., Gurley, B., & Hendrickson, H. P. (2009). Validation of a liquid chromatography–tandem mass spectrometric assay for the quantitative determination of hydrastine and berberine in human serum. Journal of pharmaceutical and biomedical analysis, 49(4), 1021-1026.
• Satelli, A., Rao, P. S., Gupta, P. K., Lockman, P. R., Srivenugopal, K. S., & Rao, U. S. (2008). Varied expression and localization of multiple galectins in different cancer cell lines. Oncology reports, 19(3), 587-594.

Developed a novel LC-MS/MS method for simultaneous therapeutic drug monitoring of four first-line anti-TB drugs using dried blood spot samples. The method is currently under clinical evaluation by Indian Council of Medical Research (ICMR) and Central Drug Standards Control Organization (CDSCO) for developing an IVD kit.