RAHUL REGHUNATH

• Operate NanoString Digital Spatial Profiler (DSP) for high-resolution spatial analysis of gene expression in tissue samples for precision medicine and disease modeling
• Design and execute spatial transcriptomics assays, optimizing sample preparation, hybridization, and data protocols
• Conduct multiplexed gene expression analysis using nCounter for RNA, miRNA, and protein assays
• Perform data analysis using GeoMx DSP and nSolver, providing insights into gene expression, immune profiling, and tissue micro-environment
• Collaborate with cross-functional teams to interpret data and contribute to therapeutic development
• Maintain DSP and nCounter systems, ensuring continued research excellence

• Lead client meetings to confirm project requirements
• Oversee lab operations and ensure quality control
• Drive digital marketing initiatives for brand visibility
• Manage sales and customer relationships for extraction kits for plants and animal tissues

• Supported senior staff with day-to-day tasks, ensuring adherence to CGLP guidelines
• Executed DNA isolation for PacBio and Nanopore sequencing in diverse tissue types
• Performed library preparations for Small RNA, Exome, MeDIP Seq, and HiC sequencing
• Managed Nanopore Mk1C and provided training for lab personnel

• Conducted DNA/RNA extraction and library prep for Illumina platforms (MiSeq, HiSeq)
• Set up reactions for Sanger sequencing and performed QC of sequencing samples
• Handled ABI 3730XL and assisted with Next-Generation Sequencing (NGS) tasks
• Managed metagenome and genotyping by sequencing projects and prepared comprehensive QC reports

• Performed QC for Sanger sequencing samples (Agarose Gel Electrophoresis, Nanodrop)
• Assisted in sample purification using various extraction kits

Project: Characteristics of Pollen-grain in Adenanthera Species