Medha Karnik S. R.

• Successfully developed a multiplex PCR-based protocol for detecting microbial dysbiosis in cervicovaginal lavage to identify women at risk for cervical cancer (Two Patents)
• Quantified inflammatory protein levels in cervicovaginal lavage using a commercially available ELISA kit to assess their correlation with disease severity
• Optimized and developed an untargeted LC-HRMS (Triple TOF 6600, Sciex) approach for identifying metabolic signatures in cervical cancer using cervicovaginal lavage
• Studied microbial diversity in cervical cancer using 16S rRNA metagenomics
• Optimized DNA extraction and PCR-based assay for detecting vaginal pathogens, including Trichomonas vaginalis, bacterial vaginosis-associated bacteria, and Candida species from cervicovaginal samples
• Performed Gram staining and Nugent scoring to evaluate bacterial vaginosis
• Administered questionnaires, obtained informed consent, and collected cervicovaginal lavage samples for the research study
• Contributed to the identification of fibronectin as a potential biomarker for TB severity assessment, with implications for early diagnosis and treatment monitoring
• Investigated the functional studies of Eupatorium glandulosum extract and its bioactive compound, 1-Tetracosanol
• Center of Excellence in Molecular Biology and Regenerative Medicine Laboratory (CEMR, A DST-FIST sponsored center and ICMR-Collaborating Center of Excellence - ICMR-CCoE).

• Quantified pluripotent stem cell markers, including OCT4, SOX2, NANOG, and KLF4, in mammospheres derived from tumor samples
• Developed a PCR assay for the detection of tick-borne diseases in dogs and horses
• Validated the PCR kit for accuracy, sensitivity, and specificity in detecting tick-borne diseases in dogs

• Collection of canine mammary tumor samples for the research study
• Cultured mammospheres from tumor samples using suspension cell culture techniques

• Assisted in developing molecular assays to study the genome instabilities
• Developed GAL1 2-Deoxy galactose system, a counter selectable marker system to determine the chromosome loss in C. tropicalis using the SAT-1 Flipping strategy

• Investigated mutations in the exonic and intronic regions of Nicotinamide Riboside Kinase 1 (NMRK1) and nicotinamide nucleotide adenylyltransferase 2 (NMNAT2), key genes in the NMN biosynthesis pathway, in Leber congenital amaurosis (LCA) patients.
• Performed Sanger sequencing for genetic analysis and mutation detection
• Conducted bioinformatics analysis to interpret sequencing data and identify potential pathogenic variants

• Successfully purified Hu protein using chromatographic techniques for structural and functional

characterization

• Performed gel filtration FPLC (Fast Protein Liquid Chromatography) to analyze the molecular weight and

oligomeric state of the purified Hu protein

• Contributed to understanding the molecular mechanisms of H. pylori nucleoid organization and DNArelated

processes