RAMAKRISHNAN G
Trichy
Summary
Expert in nanoparticle synthesis and cancer cell analysis with a focus on live tracking drug delivery systems. Skilled in using in-vitro cell models and molecular signaling techniques to target cancer cells. Proven ability to collaborate with multidisciplinary teams to develop solutions for drug-resistant cancer. Adept at translating complex data into actionable insights for research and clinical use.
Overview
7
7
years of professional experience
Work History
Doctor of Philosophy
SRM Institute of Science and Technology
Chennai
01.2021 - Current
- Created cancer cell lines resistant to doxorubicin, analyzing their characteristics through targeted gene and protein assays.
Utilized techniques including quantitative PCR, immunohistochemistry, flow cytometry, ELISA, and immunoblotting.
Formulated honeycomb-structured nanoparticles containing various therapeutic agents to inhibit resistant cancer cells.
Assessed cytotoxicity by contrasting impacts on primary healthy cells with those on resistant cells.
POSTGRADUATE PROJECT
SRM Institute of Science and Technology
Chennai
01.2021 - 07.2021
- Identified gene expression heterogeneity in rheumatoid arthritis patients through in silico analysis.
Validated results using primer design and electronic quantitative PCR methods.
Established homology with specific gene regions in affected individuals during the study.
UNDERGRADUATE mini-PROJECT
The New College
Chennai
01.2019 - 07.2019
- Isolated and identified fungal species for amylase enzyme production study.
Quantified amylase production using batch and continuous fermentation methods.
Determined yield of amylase through comparative analysis of fermentation techniques.
Education
PhD - combination nanodelivery to inhibit resistant cancer cells
SRM Institute of Science and Technology
Chengalpattu, INDIA01.2025
M.Sc. - Biotechnology
SRM Institute of Science and Technology
Chengalpattu, INDIA01.2021
B.Sc. - Biotechnology
The New College
Chennai, INDIA01.2019
DMLT -
Madras University
Chennai, INDIA01.2018
CMLT -
Madras University
Chennai, INDIA01.2017
Higher Secondary -
AGM Higher Secondary School
Eragudi, INDIA01.2015
Skills
- Nanoparticle Synthesis
- Bottom-Up Techniques
- Top-Down Techniques
- Synthesis of Mesoporous Silica Nanoparticles
- Fluorescent Nanoparticles
- Confocal Microscopy
- Flow Cytometry
- Dynamic Light Scattering (DLS)
- Scanning Electron Microscopy (SEM)
- Transmission Electron Microscopy (TEM)
- Brunauer-Emmett-Teller (BET) method
- Fourier Transform Infrared Spectroscopy (FTIR)
- X-ray Diffraction (XRD)
- UV-Vis Spectroscopy
- Animal Cell Culture Techniques
- In-silico Simulations
- In-vitro Studies
- Cell Viability
- MTT Assay
- Clonogenic Assay
- Lactate Dehydrogenase
- Intercellular ROS Measurement
- Mitochondrial Membrane Potential Measurement
- Cell Cycle Analysis
- Nuclear Staining
- Apoptosis Detection
- Immunofluorescence
- RNA Isolation
- Electrophoresis
- cDNA Synthesis
- Quantitative PCR
- Primer Design
- Protein Separation
- Cytosolic and Nuclear Separation
- SDS-PAGE
- Western Blotting
- ImageJ Software
- Data Interpretation
- Excel
- Prism Software
Hobbies and Interests
- Yoga
- Gym
- Reading
- Cooking
Projects
Undergraduate Mini-Project, 2019, 6 months, The New College, Amylase enzyme production in fungi was studied by isolating and identifying species. The production of amylase was quantified, and both batch and continuous fermentation methods were used to determine the yield. Postgraduate Project, 2021, 6 months, SRM Institute of Science and Technology, In silico analysis was conducted to identify the heterogeneity of gene expression in rheumatoid arthritis patients. This analysis confirmed the homology with gene regions in individuals and was validated through primer design, validation, and electronic quantitative PCR. Doctor of Philosophy, 2025, 48 months, SRM Institute of Science and Technology, I developed cancer cell lines that are resistant to doxorubicin and studied their characteristics through targeted gene and protein analysis. This included techniques such as quantitative PCR, immunohistochemistry, flow cytometry, ELISA, and immunoblotting. Additionally, I synthesized honeycomb-structured nanoparticles that were loaded with various therapeutic agents, including fluorescent markers, adjuvants, and chemotherapy drugs, to specifically target and inhibit the resistant cancer cells. The cytotoxicity of these treatments was evaluated by comparing their effects on primary healthy cells with those on the cancer-resistant cells.
Disclaimer
I declare that the information furnished above matches my knowledge and belief.
References
- Dr. K. Venkatesan, Associate Professor, venkatek@srmist.edu.in, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology
- Dr. S. Nageswaren, Associate Professor, nageswas@srmist.edu.in, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology
Areas Of Interest
Targeted co-delivery system to resistant cancer cells
Languages
Tamil
First Language
English
Proficient (C2)
C2
Timeline
Doctor of Philosophy
SRM Institute of Science and Technology
01.2021 - Current
POSTGRADUATE PROJECT
SRM Institute of Science and Technology
01.2021 - 07.2021
UNDERGRADUATE mini-PROJECT
The New College
01.2019 - 07.2019
PhD - combination nanodelivery to inhibit resistant cancer cells
SRM Institute of Science and Technology
M.Sc. - Biotechnology
SRM Institute of Science and Technology
B.Sc. - Biotechnology
The New College
DMLT -
Madras University
CMLT -
Madras University
Higher Secondary -
AGM Higher Secondary School
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