R Studio
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Rameshkumar G
Lead Research Associate
Bangalore,Karnataka
Summary
Hard working professional with 4+ years experience in molecular and genomics diagnosis industry as lead research associate in a CAP accredited lab. Also, a proficient tester, designer and debugger with an in-depth understanding of building and running automated liquid handling systems for sample preparation and Next-generation sequencing library preparation workflows. Reliable team player with excellent communication and problem-solving skills.
Overview
12
12
years of professional experience
5
5
years of post-secondary education
Work History
Lead Research Associate
Medgenome Labs, Ltd
Bangalore, Karnataka
04.2019 - Current
- Asia’s leading genomics and molecular CAP accredited diagnostic company
- Streamlined laboratory processes to meet tight deadlines for multiple projects.
- Controlled costs and optimized spending via restructuring of budgets for consumables, capital assets, inventory purchasing and technology upgrades.
- Expanded cross-functional organizational capacity by collaborating across departments on priorities, functions and common goals.
- Researched and selected automation tools, prioritizing cost-effective solutions to shorten diagnostic workflows.
- Validated production deployment changes, providing reports on discovered issues to manager.
- Implemented end-to-end automation for Covid19 diagnostics, reducing test costs by 20%.
- Identified areas needing improvement to review with senior leadership and develop plans for improvement.
- Successfully optimized and validated several commercial NGS library preparation in automatic liquid handler such as Biomek 4000, Agilent BRAVO and Micorlab STAR.
- Designed standardized processes and protocols in BRAVO to continuously improve operational efficiency through positive iteration.
- Recently validated and implemented end-to-end library preparation protocol for routine testing in BRAVO option B, reducing turnaround time by 2 days.
- Evaluated other liquid handling machines such Dragonfly, Epmotion and QIAgility for PCR and NGS protocols.
- Trained test technicians and operators on liquid handling machines and software.
- Completed testing and validation projects by deadline and under budget for company-wide initiatives.
Senior Research Associate
Medgenome Labs Ltd
Bangalore, Karnataka
03.2017 - 03.2019
- Executed DNA extraction for all samples related to HLA typing using automated extractor QIAsymphony and QIAcube
- Collaborated with team members to develop and implement best practices and accomplished all team goals.
- Performed routine high resolution HLA typing experiments and documented.
- Conducted RT-PCR and Sanger data analyses on regular basis.
- Set up equipment, organized inventory and maintained facilities.
- Helped team meet regulatory requirements by coordinating documentation and filings.
- Performed research into new products to increase knowledge and to provide valuable contributions.
- Led team of 5 on validation projects and successfully met key milestones
Senior Research Fellow
Madras Diabetes Research Foundation
Chennai, Tamilnadu
11.2015 - 03.2017
- Worked on Indo-Danish project, identifying gut microbiome signatures in Indian and Danish study participants associate with pre-diabetes and T2D.
- Oversaw studies, documented data and extrapolated results.
- Followed best practices and scientific protocol to reach defensible conclusions based on solid evidence.
- Performed regular Caco2 cell culture (reviving, passaging, growing and harvesting) experiments and documented findings.
- Conducted multiplex ELISA with serum and plasma for inflammatory markers using cutting edge Luminex technology.
- Worked both independently and collaboratively in fast-paced laboratory environment.
- Generated high-quality, professional papers for submission to scientific publications.
Junior Research Fellow
Institute of Genomics and Integrative Biology, CSIR-IGIB
New Delhi, National Capital Territory of Delhi
04.2013 - 11.2015
- Worked in project: Genetics and system biology of childhood obesity in India and Denmark
- Interacted with study participants by preparing for examinations, obtaining consent and collecting specimens.
- Genotyped first phase of EWAS using Affymetrix Axiom Genome-Wide EUR 1 in GeneTitan with sample size of 3000 to 4000.
- Performed whole genome imputation for fine mapping associated loci using IMPUTE2 software.
- Maintained detailed records of project data and materials, updating information when changes arose.
- Recorded data from research to allow for accurate analysis.
- Computed complex statistical analyses and interpreted data using R studio and PLINK.
- Initiated, planned and executed research projects under supervision of senior scientist.
- Communicated project status to principal investigator through technical documentation and presentations.
Research Assistant
Masila’s Cancer Diagnostics Pvt.Ltd
Chennai, Tamilnadu
05.2007 - 05.2010
- Planned, modified and executed research techniques, procedures and tests.
- Validated blood and specimen collection orders, alerting nurses or physicians of discrepancies between order and nursing station logs.
- Evaluated cancer biomarkers using fluorescence spectroscopy in blood & urine.
- Developed macros, special formulas and other actions to produce reliable and consistent statistical reviews.
- Coordinated with lab technicians and nurses, for two Clinical trial studies conducted in parallel with two different cancer hospitals
- Participated in Medical camp in Periyapattinam village (Ramnad), Tamilnadu, India in July 2007, about Cancer Screening and Awareness Program.
Education
M.Sc - Molecular biology
University of Madras
Chennai 04.2010 - 04.2012
B.Sc - Biochemistry
Periyar University
Salem 04.2004 - 05.2007
Skills
- NGS library preparation
Accomplishments
- Awarded as Star employee of the year 2020 and member of Medgenome Elite club
- Contributed to the quality control and quality assurance team and which lead to successfully recognition by various accreditation agencies such as National Accreditation Board for Testing and Calibration Laboratories (NABL – India), The college of American pathology (CAP), The European Federation for Immunogenetics (EFI) and pharmaceutical companies
- Co-authored 5 peer reviewed journals published in Genome Medicine, PLoS ONE etc with a cumulative impact factor 37
Software
SAS Base
Timeline
Lead Research Associate
Medgenome Labs, Ltd
04.2019 - Current
Senior Research Associate
Medgenome Labs Ltd
03.2017 - 03.2019
Senior Research Fellow
Madras Diabetes Research Foundation
11.2015 - 03.2017
Junior Research Fellow
Institute of Genomics and Integrative Biology, CSIR-IGIB
04.2013 - 11.2015
M.Sc - Molecular biology
University of Madras
04.2010 - 04.2012
Research Assistant
Masila’s Cancer Diagnostics Pvt.Ltd
05.2007 - 05.2010
B.Sc - Biochemistry
Periyar University
04.2004 - 05.2007
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