Ramkishan Gaddam
Biotechnologist
Hyderabad
Summary
To work in an organization where people are always encouraged to generate a continuous stream of innovative ideas and to evolve new perspective in the multifaceted areas of challenging communication environment.
Overview
18
18
years of professional experience
3
3
Languages
Work History
Research Associate
Riya Argo Biotech Company
04.2023 - Current
- Working on Banana Tissue culture and Marketing.
SRF and JRF
Cell, Molecular Biology & Trait Engineering Laboratory, Accelerated Crop Improvement Program, ICRISAT
02.2013 - 03.2023
- Working on regeneration and Genetic transformation of chickpea, molecular characterization of putative transgenics and bioassay for insect resistance.
Part Time Lecturer
Nizam College
01.2012 - 01.2013
- Teaching in Department of Botany for UG course.
Lecturer
Gaayathri Degree & PG College
01.2006 - 01.2010
- Teaching in Department of Botany for both UG & PG courses.
Education
M.Tech (Bio-Tech) -
Jawaharlal Nehru Technological University
HyderabadMaster’s Degree in Botany - Cytogenetics, Genetics and Molecular Genetics
Osmania University
Bachelor of Science - undefined
Kakatiya University
Skills
Genomic DNA & RNA isolation from plants
Plasmid DNA isolation by using kit and manual method
Protein Extraction from plants & Yeast
Nucleic acids quantification by using qubit and Spectrophotometer
Agarose Gel electrophoresis
SDS-PAGE
Gel extraction and PCR product purification
Polymerase chain reaction (PCR)
cDNA synthesis
Restriction digestion
competent cells preparation
Transgenic analysis by using PCR
Tissue culture
Media and stocks preparation
Multiple Shoot Induction
Shoot Elongation induction
Root induction
Argo-bacterium mediated transformation
Maintenance of transgenic events in green house
Microbiology
Glycerol Stock preparation
Maintenance of Bacterial cultures (streaking)
Biochemical Techniques
ELISA for the quantitative detection of Cry2Aa
Bioinformatics Tools
Biological sequence retrieval from NCBI & Sequence analysis
Primer designing
Entomology
Insect Bioassay
Contained field trials
Personal Information
- Father's Name: G. Shankaraiah
- Date of Birth: 06/04/1982
- Gender: Male
- Nationality: Indian
- Marital Status: Married
Awards
GATE qualified, 2009, 87.73 percentile
Publications
- J. B. Nayak
- R. Sunil kumar, G. Ramkishan, Larvicidal Efficacy of Annona squamosa Plant Extracts Against Culex Quin quefasciatus Mosquito Larvae, International Journal of Scientific Research, 4, 7, 141-142, 2015
Courses Workshops Attended
- IPPC-2019, 5 days, Hyderabad
- IAPPS & ICRISAT Training Programmed to PG Teachers of Affiliated colleges, 5 days, Warangal
- Molecular & Phytochemical Techniques in Plant Sciences, 6 days, Warangal
- National Conference on Current Trends in Plant Sciences, 2 days, Warangal
- Training Programme to PG Teachers of Affiliated colleges, 5 days, Warangal
- International Conference on Plant Biotechnology & Molecular Biology, 3 days, Warangal
- National Conference on Plant Sciences in human welfare, 2 days, Warangal
References
- Dr. D. Srinivas Reddy, Lead Scientist, ATGC Biotech Pvt Ltd, Hyderabad, dsreddy@atgc.in, +91 9849049205
- Dr. M Aruna, Professor, Dept. of Botany, Telangana University, Nizamabad, aruna_maddela@yahoo.com, +91 9849049205
Projects
Genetic Transformation of Chickpea for Insect Resistance, Chickpea (Cicer arietinum L.) is an annual, self-pollinated grain legume crop. About 80-90 percent of total pest damage observed in chickpea is due to a single pest that is gram pod borer, Helicoverpa armigera (Hubner), (Lepidoptera). This pest has acquired resistance to almost all kinds of insecticides to varying degrees. So, the need for development of in-built resistance to the H.armigera has taken a vital role in crop improvement. In the present study axillary meristem explants from the invitro germinated seedlings of chickpea cultivar JG11were co-cultivated with Agrobacterium tumefaciens LBA4404 strain harboring the vector pGreen29-cry2Aa under the control of the constitutive 35S CaMV promoter were used to develop the transgenic chickpea plants with insect resistance. Molecular analysis of the putative transformants by PCR revealed the presence of the cry2Aa gene. Further, ELISA tests confirmed the protein expression in transgenic events.
Disclaimer
I hereby declare that the information furnished is true and correct to the best of my knowledge and belief.
Timeline
Research Associate
Riya Argo Biotech Company
04.2023 - Current
SRF and JRF
Cell, Molecular Biology & Trait Engineering Laboratory, Accelerated Crop Improvement Program, ICRISAT
02.2013 - 03.2023
Part Time Lecturer
Nizam College
01.2012 - 01.2013
Lecturer
Gaayathri Degree & PG College
01.2006 - 01.2010
M.Tech (Bio-Tech) -
Jawaharlal Nehru Technological University
Master’s Degree in Botany - Cytogenetics, Genetics and Molecular Genetics
Osmania University
Bachelor of Science - undefined
Kakatiya University