Renu Mulik

• Actively participated in the development of the bacterial colitis model and the study of brain-gut axial dysbiosis in the colitis model (c57BL/6) by rectal administration of five pathogenic organisms, including Enterococcus faecalis, E. coli, Pseudomonas aeruginosa, Streptococcus mutans, Proteus mirabilis, and the cocktail of organisms
• Performing the behaviour study Morris water maze, open field test, T maze, Y maze, Novel object recognition (NOR),Elevator plus maze
• Performing the anaesthesia, cardiac puncture and sac collection of the brain, spleen, kidney, heart, ilium
• Caring out molecular study RT-PCR, IHC (Immunohistochemistry), western blotting , SDS
• Additionally, studying the effect of bile acid deoxycholic acid and propionate (anti-apoptosis and pro-apoptosis) in the PC12 and N2a cells using the MTT assay
• Assisted three PhD scholars in animal behavior (c57BL/6) in performing Morris water maze, T maze, Y maze, Novel object recognition (NOR), Elevated plus maze and oral gavage,revised neurobehavioural severity test (NSS-R) test and collecting the faecal sample, collection of blood by cardiac puncture
• Proficient in culturing and maintaining cancer cell line (HOS, HEK) neuronal cell line ( PC12, N2a), primary cell culture, tissue culture SVZ and DG
• Assisting the phd scholar in the culturing multipotent neural stem/progenitor cells from dentate gyrus and subventricular zone of a single adult mouse
• With a strong background in cell biology, I have skillfully handled thawing and the subsequent revival of frozen cell cultures
• SDS page ,Western Blotting of the Hif1a, Beta actin gene and Immunocytochemistry (ICC) of the Subventricular zone
• Proficient in isolating RNA from various samples and performing c-DNA synthesis for the gene expression analysis
• Conducting the RT-PCR experiment to analyse gene expression patterns of virulence genes
• Performed a range of cell-based assays, such as MTT assays, Fluorescence imaging and Western Blotting assays to assess the efficacy of the drug
• Studied FACS to investigate the cell cycle dynamics of N2a and PC12 cells
• Utilizing fluorescent markers flow cytometry and propidium iodide to gain the insights into interpreting the movement of cells through distinct phases of their life cycle
• Also illuminates the intricate regulatory pathways involved in cell division and expansion for these distinct cell cultures
• By combining these techniques, gain a deeper understanding of important cellular functions and their applications in neuroscience
• The review article entitled "The role of secondary bile acids deoxycholic acid and lithocholic acid in the unfolding protein response (UPR) and Endoplasmic reticulum stress activation of the down regulatory cascade." is ready for submission
• Colony qPCR (MDA-T32) thyroid cancer and performed the gel electrophoresis

• Laboratory animal science animal welfare and ethics Dr.Vijay pal.Singh
• Legislation in India-On Lab Animal Science & Experimentation Dr.Vivek Tyagi,Expert Consultant, CPCSEA
• Practical Training: Handling and Restraining of Mice ,Rar,Guinea Pig and Rabbit
• Anatomy of lab Animal Dr.Inderjeet Yadav,NBRC
• Birds in neuroscience research Dr.Soumya Iyengar,NBRC
• Perioperative care and analgesia Crucial role of laboratory animal models in stem cell research and regenerative medicine Dr.Taru Sharma Director,NIAB,Hyderabad
• Good welfare make good science” -Vera Baumans Utrecht University,TheNetherlands
• Nutrition and Feeding -Dr.Johannes Cornelius Meijer, Utrecht University ,The Netherlands
• Animal Models of osteoporosis,rheumatoid arthritis and osteoarthritis Dr.Mohan Wani,Director,NCCS
• Arrive and Prepare guidelines -Dr.Anupama,ILBS
• Transgenics and Genome Editing Technology:Mechanism,Applications and future prospects
• Guidelines for filling form B and other regulatory aspects.-Dr.Kripale Pandae,Expert consultant,CPCSEA,Dr,Siddharth Pandey
• Demonstration of experimental techniques :Collection of body fluids and Injection techniques (oral gavage, ip,im,iv)
• Demonstration of experimental techniques: Suturing
• Genetic standardization to maintain pure strains of Laboratory rodents -Dr.Bela Desai,DB
• Animal Models for poultry research -Dr Debasis Nayak IISER,Bhopal
• Occupational health Hazards and Safety-Dr.Ramachandra S.G,Director,NARFBR,Hyderabad
• Basics of Rodent Anaesthesia,Surgery and Perioprative care, Hands-on Assessment
• Basic of surgery & Anesthesia-Dr.Sushil Kumar Bits Pilani
• Drug repurposing in pre- clinical research- Dr Somdatta Sen Saphera Pharma
• Rodent Necropsy for data generation- Dr.Anupama,ILBS
• Manipulation of gene expression in Hippocampus and analysis of spatial memory-Dr.Sourav Banerjee NBRC
• Perfusion techniques, Security assessment and humane endpoints - A Practical approach to implement refinement in experimental procedure
• Alternative in Animal Experimentation( Knowledge of tumor organoid model (PDTO), Spheroid, 3D model under the guidance of )- Dr.Suresh Poosala
• Cell culture method for understanding brain disorder -Dr.Pankaj Seth NBRC
• Animal Model for screening new drugs in Cardiovascular Research-Dr.Harlokash,AIIMS New Delhi
• History of animal welfare in india -Dr.Sujoy Khanna LUVAS,Hisar
• Effective Health Monitoring,Diseases and Pathology Dr.Nagarajan,NII,New Delhi
• Necropsy Procedure & handling animal tissues for Histo-pathological examination
• Laboratory animal facility design and equipments-Dr.Shital Suryavanshi TIFR,Mumbai
• Zebrafish as the vivo model system : From basics of rearing to applications in Biomedical research-Dr.Rajender Motiani,RBC,Faridabad
• Laboratory animal import,breeding strategies and supply for experiments- Dr.Shital Suryavanshi TIFR, Mumbai
• AALAS International Standards and accreditation Process-KB Patel ILBS,New Delhi, Introduction to GLP guidelines Protocol Preparation & Self Study - KB Patel ILBS, New Delhi
• Presentation of protocols - DR Anirban Basu/ Dr.Sourav Banerjee / Dr.Inderjeet/Dr.Vijaypal
• Valedictory function and Certificate Distribution - Dr.S.K Dutta,Joint Commissioner (Animal Welfare)& (Member Secretary (CPCSEA)
• I also explore the research work on learning and memory in zebra finches and crows under the supervision of Dr Soumya Iyengar
• The work presentation of of Dr.Anindya Ghosh-roy, investigated the work of nervous system development and injury repair in C Elegans
• Exploring the study of glial cells i.e astrocytes and oligodendrocytes inducing zika virus and brain fog under the supervision of pankaj seth lab.

• During a Tenure as a trainee under the mentorship of Dr Ilpy Cherian in the neurosurgery department:
• I acquired practical skills in neuroimaging techniques, encompassing MRI, T1, T2, and FLAIR Image analysis
• I actively participated in Deep Brain Stimulation tractography with the integration of augmented reality technology
• I contributed to patient evaluations and collaborated closely with the medical team to formulate treatment strategies
• I conducted extensive literature reviews and research to support ongoing neurosurgical initiatives
• I was involved in cutting-edge research focused on leveraging augmented, mixed, and virtual reality for Deep Brain
• Stimulation tractography, enhancing the visualization of white matter in patients with diverse tumour profiles
• I possess expertise in neuroimaging techniques, encompassing Magnetic Resonance Angiography (MRA), Magnetic
• Resonance Imaging (MRI), and Computed Tomography (CT), facilitating comprehensive neurological diagnosis and research.

Hosted Neurosurgery International Conference – 27th -29th Jan 2022

● “2nd Annual Kims International Neurosurgery Conference & Inauguration of Aesculap Neurosurgical skill lab at Krishna Institute of Medical Science Deemed To Be University " 27 th -29th January , KIMS DU college,KARAD,MAHARASHTRA

● Coordinating guest and faculty logistics, managing registrations, funds ,overseeing the gala program, securing a suitablevenue, arranging accommodations and travel, and executing scientific sessions with utmost care were part of ourmeticulous planning.