Sadhana Singh Sagar
Hyderabad
Summary
Experienced Ph.D. scientist in microbiology and infectious disease biology, specialising in experimental design and assay optimisation, cell culture, and partly work on enteroid organoids. Developed advanced methodologies in CRISPR-Cas biology and Molecular biology techniques, executing complex laboratory projects and analysing biological data. Committed to advancing translational life-science R&D and small-molecule discovery.
Overview
2
2
Languages
17
17
years of professional experience
Work History
Biologist
Guidehouse & National Institutes of Health (NIH)
08.2023 - 11.2025
- Produced virus-like particles for Norovirus strains
- Genotyped patient stool samples to identify Norovirus and determine patient secretor status, enhancing understanding of viral infection patterns.
- Execute infectious-disease research workflows with emphasis on reproducibility, assay performance and interpretation of experimental results.
- Demonstrated expertise in molecular, RNA, and cell biology techniques through hands-on research and experimentation.
- Maintained rigorous documentation of experimental procedures and results, upholding high standards of scientific integrity.
- Assisted in protocol development and implementation for endroid organoids (EOs) at NIH, facilitating advanced research capabilities.
Research Volunteer
Georgetown University
Washington, DC
05.2022 - 08.2023
- Conducted research on Hepatitis Delta Virus, contributing to understanding of viral dynamics under Prof. John Casey.
- Supported molecular and infectious disease research in an interdisciplinary laboratory, facilitating collaboration and innovation.
- Mentored students to support achievement of target grades.
- Attended conferences to learn about new research and present findings.
- Supervised student projects, facilitating progress and encouraging deeper learning through application.
Scientist B / Permanent Faculty
Center for Water Resources Development and Management
Kozhikode
07.2019 - 04.2022
- Completed PI-level project planning for control of waterborne pathogens, integrating bacteriophage cocktails with herbal formulations to improve intervention efficacy.
- Led applied research on bacteriophage-mediated control of waterborne bacterial pathogens.
- Developed and evaluated experimental approaches for quorum sensing and antimicrobial interventions, enhancing pathogen mitigation strategies.
- Investigated quorum-sensing mechanisms in Vibrio cholerae, Escherichia coli and Salmonella typhimurium, evaluating essential-oil-based interventions to inform future pathogen control methods.
Postdoctoral Fellow
South China Agricultural University
Guangzhou
01.2019 - 12.2019
- Investigated CRISPR-Cas systems to understand their role in reversing antibiotic resistance in Pseudomonas aeruginosa UCBPP PA14.
- Analysed and interpreted complex datasets, drawing meaningful conclusions to inform research.
- Coordinated safe and successful laboratory experiments designed to test theories proposed in classes.
- Coordinated with senior researchers to design research methodologies, ensuring adherence to project objectives.
Teaching / Research Associate, Microbiology
Rani Lakshmi Bai Central Agricultural University
Jhansi
01.2016 - 12.2019
- Prepared bacteriophage cocktails from potent isolated phages, assessing their effectiveness against multidrug-resistant pathogens to support development of targeted therapies.
- Conducted research on bacteriophage-based pathogen control, phage resistance, bacterial virulence, and antimicrobial strategies to advance understanding of microbial interactions.
- Synthesised nanoparticles using lytic bacteriophages, evaluating their efficacy against bacterial pathogens to contribute to innovative treatment solutions.
Guest Faculty, Biochemistry
Veer Bahadur Singh Purvanchal University
Jaunpur
01.2015 - 12.2016
- Delivered lectures in immunology, microbiology, animal physiology, and plant physiology, mentoring postgraduate students in dissertation research to foster academic development.
- Collaborated with team members to achieve target results.
- Managed complaints with calm, clear communication and problem-solving.
- Maintained clean, safe working environments to eliminate accident risks.
Researcher
Department of Microbiology, CSJM University
Kanpur
01.2009 - 12.2011
- Investigated biofilm formation, antimicrobial resistance, and virulence characteristics of bacterial isolates to understand their impact on infection control.
- Conducted research on heavy-metal-remediating bacteria to identify potential bioremediation applications in environmental management.
- Collaborated with colleagues on research assignments to inform strategy.
- Presented oral and written analysis at meetings and conferences to share research outcomes.
- Conducted in-depth research utilising various techniques, including qualitative and quantitative methods.
Education
Ph.D. - Environmental Microbiology
Babasaheb Bhimrao Ambedkar University
Lucknow, India01-2016
M.Phil. - Microbiology
Vinayaka Missions University
Salem, Tamil Nadu, India01-2009
M.S. - Microbiology
Chhatrapati Shahu Ji Maharaj University
Kanpur, India01-2008
Skills
- Virus-like particle production
- CRISPR-Cas research
- Cell culture
- Enteroid organoids
- Molecular biology and diagnostics
- Pathogen genotyping
- Assay optimisation
- Antimicrobial screening
- Biofilm assays
- Quorum-sensing assays
- RNA Biology
- Bacterial characterization
- Genetic analysis
- Phage-based antimicrobials
- Phage-antibiotics
- Norovirus
- Hepatitis Delta Virus research
- Pseudomonas aeruginosa
- Assay development
- MDR pathogens
- Grant Writing
- Data interpretation
- Project coordination
Custom
extensive experience developing and optimizing microbiological, biofilm, quorum-sensing, antimicrobial and pathogen-control assays., research experience involving CRISPR-Cas systems, bacterial genetics/phenotyping, pathogen characterization and molecular diagnostics., experience evaluating antimicrobial candidates, bacteriophages, antibiotics, essential oils and nanoparticle-based interventions., current NIH work on Norovirus patient samples, secretor status and virus-like particle/HBGA interaction studies., PI-level project experience, experimental planning, troubleshooting, data interpretation and scientific reporting., research spanning antimicrobial discovery, antibiotic resistance, diagnostics, pathogen control and environmental/public-health applications.
Languages
English
Advanced
C1
Hindi
Advanced
C1
Timeline
Biologist
Guidehouse & National Institutes of Health (NIH)
08.2023 - 11.2025
Research Volunteer
Georgetown University
05.2022 - 08.2023
Scientist B / Permanent Faculty
Center for Water Resources Development and Management
07.2019 - 04.2022
Postdoctoral Fellow
South China Agricultural University
01.2019 - 12.2019
Teaching / Research Associate, Microbiology
Rani Lakshmi Bai Central Agricultural University
01.2016 - 12.2019
Guest Faculty, Biochemistry
Veer Bahadur Singh Purvanchal University
01.2015 - 12.2016
Researcher
Department of Microbiology, CSJM University
01.2009 - 12.2011
Ph.D. - Environmental Microbiology
Babasaheb Bhimrao Ambedkar University
M.Phil. - Microbiology
Vinayaka Missions University
M.S. - Microbiology
Chhatrapati Shahu Ji Maharaj University