Sahista Talukdar
Hyderabad
Summary
- Highly motivated and dynamic individual with 12 years of experience in drug metabolism and pharmacokinetics.
- Expertise in invivo ADME, mammalian cell line handling, cell-based assays, and troubleshooting. Able to quickly learn new techniques and research methods.
- Skilled in leading, motivating, multitasking, and organizing team members for effective outcomes.
- A team player with innovative thinking and problem-solving abilities.
- Got certified with udemy for clinical data management course.
Overview
13
13
years of professional experience
1
1
Certification
Work History
Principle Scientist
Pharmajen Pvt Ltd
Hyderabad
07.2024 - Current
- Developed and conducted experiments to study- in vitro.
- Maintained accurate records of experimental protocols and results in accordance with regulatory requirements.
Associate Research Scientist
Sai Life Science Pvt ltd
Hyderabad
11.2021 - 07.2024
- Cell line handling & maintenance, Cell revival, Splitting, Freezing, Mycoplasma detection, Record and log book maintenance for cell biology lab
- Cell based assays, Cell culture techniques and their troubleshooting, Handled and used various mammalian cell lines for Invitro assays (e.g., HepG2, Caco-2, MDCK) Established drug transport and permeability of across biological barriers using invitro system including CACO-2 and MDCKII and MDCKII-MDr 1 cell line model
- Developed a team of junior scientists for permeability assay, cell maintenance and cell culture activity
- Developed Pgp and BCRP transport Substrate and Inhibitor assessment using CACO-2 cell line
- Established permeability assay using Bovine serum albumin in MDCKII MDR1 cell line
- Designed and Responsible for mammalian cell line (C ACO-2 and MDCKII) seeding on routine basis maintain them for respective time incubation and performing permeability experiment with data interpretation and trouble shooting
- In- vitro half-life or clearance rate of NCE drug in hepatocyte using multiple species
- Cytotoxicity assays by using MTT in cell lines like Hep G2
- Managed one project to ensure release quality data of multiple assays within time line.
Associate Scientist
Syngene International Limited
06.2020 - 11.2021
- Conducting in vitro studies of New chemical entities for Binding assay such as Ultracentrifugation based hepatocyte binding assay, Ultracentrifugation based microsomal binding assay, Ultracentrifugation based brain tissue binding assay, Ultracentrifugation based Plasma Protein binding assay and Ultracentrifugation based Media(RPMI-1640) binding assay
- Troubleshooting in experimental work in a day to day activity and also help to other team member.
Senior Executive
Cliantha Research Ltd
10.2019 - 05.2020
- Method validation and sample analysis for cell based NAb assay using L929 cell line for Adalimumab drug
- Method development and validation of NK cell activity for cytotoxicity assay using K562 cell line
- Sample analysis for TFPI using ELISA method.
Research Scientist
Piramal Pharma Solutions Pvt Ltd
06.2012 - 10.2019
- Invitro-ADME: Conducting in vitro studies of New chemical entities such as Physico-chemical properties (Aq
- Solubility by UV measurement and LC-MS/MS detection, Partition coefficient(Log P), Distribution co-efficient(Log D)), Permeability by PAMPA method, Inhibition of CYP 450 enzymes (3A4, 2D6, 1A2, 2C19, 2C9,2B6 & 2C8 by fluorometric and HLM based methods, Plasma protein binding, plasma/blood stability, in- vitro half-life or clearance rate of NCE using liver microsomes,S9 fraction and hepatocyte stability from multiple species
- Chemical Stability (SGF, FASSIF, FESSIF) and using different bio relevant media
- Designed and Responsible for Time dependent based CYP inhibition and CYP inhibit ion using cocktail substrate for 7 isoforms (3A4, 2D6, 2C9, 2C19, 1A2, 2A6 & 2B6) for Phase1
- Responsible for UGT inhibition for 5 isoform (UGT1A1, UGT1A3) for Phase-2
- Designed and Responsible for mammalian cell line (CACO2, MDCK and LLC_PK1 ) s Seeding on routine basis maintain them for respective time incubation and performing g permeability experiment with data interpretation and trouble shooting
- Regular screening of compounds, data generation, compilation and report preparation on and preparation of different detailed assay procedure SOP for all ADME assay and Cell based assay
- Cell-Culture: - Cell line handling & maintenance, Cell revival, Splitting, Freezing, Mycoplasma detection, Record and log book maintenance for cell biology lab
- Cell based assays, Cell culture techniques and their troubleshooting, Handled and used various mammalian cell lines for Invitro assays (e.g
- HepG2, THP-1, Caco-2, MCF-7, MDCK, HEK-293.) Cytotoxicity assays by using MTT & CCK-8 in cell lines like THP-1, Hep G2, and HEK- 293, all 10 different cell line of different tissue type.Performed ELISA assay whenever required
- Cytokine (TNFα and IL6) Inhibition study of various drugs on THP1
- MDR1 gene Transfected in mammalian cell line MDCK
- Using CACO2 cell line model for different assay like bidirectional, unidirectional assay, BCRP substrate inhibition, Pgp inhibition and substrate assay
- MDCK and LLCPK1 using cell line model for permeability.
QC Chemist
Leamak Healthcare pvt ltd
Ahmedabad
06.2011 - 05.2012
- Responsible for QC test for API drug and related document preparation.
- During my period, I have able provide support to team member and company for Successfully got WHO Certify
Education
M.Sc. in Biochemistry -
Charotar Institute of Applied Sciences
05.2010
B.Sc. in Biochemistry -
St. Xavier's College
06.2008
12th Std -
Ankur School
05.2005
Skills
- Communication
- Presentation
- Negotiation
- Multitasking
- Positive approach
- Laboratory management
- Project management
- Experimental design
- Cell Culture
- Experiment protocol development
- Time management
- Adaptability
- High throughput screening
- Data documentation
- Teamwork
- Supervision
- Guidance
- Procedure planning
- Resource management
Certification
- Participated in workshop on DNA Purification and RADP for Fingerprinting organized byFICCI-EU-GTZ Biotech Resource Center & Conducted by Xavier Research Foundation
- LCMS - Piramal Discovery Solution Pvt Ltd
- Fundamental of Mass spectrometer by ABSCIEX INDIA
- Quantification of the impurity with Waters LCMSMS, XEVO technology by Waters.
- Got certified with udemy for clinical data management course
Publications
- 1. "Design, Synthesis, In Silico Studies and In Vitro Anticancer Activity of 3-(4-Methoxyphenyl)azetidine Derivatives(Dec-2020) Deepa R. Parmar, Rahul H. Rayani, Anand G. Vala, Rakesh V. Kusurkar, Roshani K. Manvar, Sahista N. Talukdar, Preeti, Vishwanath Zunjar, Satyanarayana Battula, Jigar Y. Soni
- 2. "Discovery of new anticancer thiourea-azetidine hybrids: design, synthesis, in vitro antiproliferative, SAR, in silico molecular docking against VEGFR-2, ADMET, toxicity, and DFT studies" July 21,2021, Elsevier Bioragnaic Chemistry .(July 2021) Deepa R. Parmar, Jigar Y. Soni, Ramakrishna Guduru, Rahul H. Rayani, Rakesh V. Kusurkar, Anand G. Vala, Sahista N. Talukdar, Ibrahim H. Eissa, Ahmed M. Metwaly, Ahmed Khalil, Vishwanath Zunjar, Satyanarayana Battula
Projects
- College Student, 06/2003, 05/2010, Participated in workshop on DNA Purification and RADP for Fingerprinting organized by FICCI-EU-GTZ Biotech Resource Center & Conducted by Xavier Research Foundation.
- Fundamental of Mass spectrometry by ABSCIEX INDIA. Quantification of the impurity with Waters LCMSMS, XEVO technology by Waters.
- Characterization of Bio control and Aromatic Hydrocarbon Utilizing Abilities of Pseudomonas aeruginosa P4" under supervision of Dr. Aditi Buch, Charotar Institute of Applied Sciences, Gujarat University
Languages
- English
- Hindi
- Gujarati
Accomplishments
Best performance of the year
Piramal Discovery Solution Pvt Ltd
I have got 2 awards for best performance of the year from Piramal Discovery Solution.
Sai Lifescience PVT Ltd
Good work appreciation award by client
Affiliations
- " Design, Synthesis, In Silico Studies and In Vitro Anticancer Activity of 3‐(4‐
Methoxyphenyl)azetidine Derivatives(Dec-2020) Deepa R. Parmar, Rahul H. Rayani, Anand G. Vala, Rakesh V. Kusurkar, Roshani K. Manvar, Sahista N. Talukdar, Preeti, Vishwanath Zunjar, Satyanarayana Battula, Jigar Y. Soni
2. " Discovery of new anticancer thiourea-azetidine hybrids: design, synthesis, in vitro antiproliferative, SAR, in silico molecular docking against VEGFR-2, ADMET, toxicity, and DFT studies" July 21,2021, Elsevier Bioragnaic Chemistry .(July 2021) Deepa R. Parmar, Jigar Y. Soni, Ramakrishna Guduru, Rahul H. Rayani, Rakesh V. Kusurkar, Anand G. Vala, Sahista N. Talukdar, Ibrahim H. Eissa, Ahmed M. Metwaly, Ahmed Khalil, Vishwanath Zunjar, Satyanarayana Battula
DECALARTION
- I hereby declare that the above written particulars are true to the best of my knowledge and belief.
References
References available upon request.
Timeline
Principle Scientist
Pharmajen Pvt Ltd
07.2024 - Current
Associate Research Scientist
Sai Life Science Pvt ltd
11.2021 - 07.2024
Associate Scientist
Syngene International Limited
06.2020 - 11.2021
Senior Executive
Cliantha Research Ltd
10.2019 - 05.2020
Research Scientist
Piramal Pharma Solutions Pvt Ltd
06.2012 - 10.2019
QC Chemist
Leamak Healthcare pvt ltd
06.2011 - 05.2012
M.Sc. in Biochemistry -
Charotar Institute of Applied Sciences
B.Sc. in Biochemistry -
St. Xavier's College
12th Std -
Ankur School
Similar Profiles
Randhir Kumar PrasadRandhir Kumar Prasad
Stacker at Engineering and Allied Services Pvt. Ltd, Lift & Shift India Pvt. Ltd, Roadwings International Pvt. Ltd, Western Carriers India Pvt. Ltd, Tractor India Pvt. Ltd, Transworld Terminal Pvt. LtdStacker at Engineering and Allied Services Pvt. Ltd, Lift & Shift India Pvt. Ltd, Roadwings International Pvt. Ltd, Western Carriers India Pvt. Ltd, Tractor India Pvt. Ltd, Transworld Terminal Pvt. Ltd
Ahmad Shah BarakzaiAhmad Shah Barakzai
Internship Student at Caribbean Infocom India Pvt. Ltd (CII Pvt. Ltd)Internship Student at Caribbean Infocom India Pvt. Ltd (CII Pvt. Ltd)
SOMANATH ANAND NAIKSOMANATH ANAND NAIK
Purchase Manager,B2B Manager,Bsv Depo Manager at SK GROUP-SKITES PHARMA PVT LTD & SKANT INDIA PVT LPurchase Manager,B2B Manager,Bsv Depo Manager at SK GROUP-SKITES PHARMA PVT LTD & SKANT INDIA PVT L
Narayanasamy VelusamyNarayanasamy Velusamy
Associate Director at Novartis PharmaceuticalsAssociate Director at Novartis Pharmaceuticals
Kiran PanigrahiKiran Panigrahi
Director - Customer Success at ModMed IndiaDirector - Customer Success at ModMed India