Samyuktha Lakkireddy

To obtain a suitable position in a renowned healthcare/pharmaceutical industry using critical thinking, communication, team work skills, and ability to work independently.

Thesis title: Role of genetic mutations and differential gene expression profiling in Chronic Myeloid Leukemia (CML) patients. Investigation of gene mutations in signaling and metabolic pathways and analyzed their role in the development, progression, and therapy response of CML patients

Expertise in Molecular Biology Techniques including:

• Mutational analysis of genes by Genomic DNA isolation, Spectrophotometric techniques, Agarose gel electrophoresis, PCR-RFLP approach and sequencing
• Pharmacogenomics study
• Analysis of differential gene expression using quantitative Real-Time PCR (qRT-PCR) of angiogenesis genes in CML patients and healthy individuals
• Chromosomal analysis by Karyotyping,
• In silico studies using Bioinformatic tools

Expertise in Immunological techniques including: Protein extraction, Characterization and Purification using Chromatographic techniques, SDS-PAGE; Plasmid extraction, Transformation, Conjugation, Clinical biochemical tests, ELISA 

Microbiological techniques: Plating techniques, Cell viability tests, Staining techniques, Micrometry, Enzymatic assays 

Statistical analysis: Fischer's exact test, Student T-test, Logistic Regression analysis, Odds ratio calculations using SNPstats, Multifactor Dimensionality Reduction Analysis (MDR), Spearmann Rank Correlation Coefficient

• Role of Angiogenesis in Chronic Myeloid Leukemia, Samyuktha Lakkireddy, Sangeetha Aula, Atya Kapley, Vedanta Narasimhaswamy Adimadhyam, Kaiser Jamil, International Journal of Recent Scientific Research, 2014, 5, 1311-1321, 5.971
• Association of The Common CYP1A1
• 2C Variant (Ile462Val Polymorphism) with Chronic Myeloid Leukemia (CML) in Patients Undergoing Imatinib Therapy, Samyuktha Lakkireddy, Sangeetha Aula, A.V.N. Swamy, Atya Kapley, Raghunadha Rao Digumarti, Kaiser Jamil, Cell Journal (Yakhteh), 2015, 17, 510-519, 2.479
• Association of Vascular Endothelial Growth Factor A (VEGFA) and its Receptor (VEGFR2) Gene Polymorphisms with Risk of Chronic Myeloid Leukemia and Influence on Clinical Outcome, Samyuktha Lakkireddy, Sangeetha Aula, Atya Kapley, A.V.N. Swamy, Raghunadha Rao Digumarti, Vijay Kumar Kutala, Kaiser Jamil, Molecular Diagnosis and Therapy, 2015, 20, 33-44, 4.074
• Association of DNA repair gene XPC Ala499Val (rs2228000 C>T) and Lys939Gln (rs2228001 A>C) polymorphisms with the risk of chronic myeloid leukemia: A case-control study in a South Indian population, Lakkireddy S, Aula S, Kapley A, Gundeti S, Kutala VK, Jamil K., J Gene Med., 2021, 23, 7, e3339, 10.1002/jgm.3339, 4.565
• In Silico Docking Studies of Vascular Endothelial Growth Factor-A (VEGFA): Possible Implications in Chronic Myeloid Leukemia (CML) Therapy, Lakkireddy Samyuktha, Jayaraman Archana, Aula Sangeetha, Kapley Atya, Kutala Kumar Vijay and Jamil Kaiser
• Current Proteomics, 2021, 18, 4, 0.837
• Biological Interactions In Vitro of Zinc Oxide Nanoparticles of Different Characteristics, Sangeetha Aula, Samyuktha Lakkireddy, A.V.N. Swamy, Atya Kapley, Kaiser Jamil, Tata Narasinga Rao, Kaliyan Hembram, Materials Research Express, 2014, 1, 035041, 0.968
• Biophysical, biopharmaceutical and toxicological significance of biomedical nanoparticles, Sangeetha Aula, Samyuktha Lakkireddy, Kaiser Jamil, Atya Kapley, A.V.N. Swamy, Harivardhan Reddy Lakkireddy, RSC Advances, 2015, 5, 47830, 3.84
• Route of administration induced in vivo effects and toxicity responses of Zinc Oxide nanorods at molecular and genetic levels, Sangeetha Aula, Samyuktha Lakkireddy, Atya Kapley, Rakesh Kumar Sharma, Shantveer G Uppin, Kaiser Jamil, International Journal of Nano Dimension, 2018, 9, 158-169, 0.766
• In Vivo Interactions of Nanosized Titania Anatase and Rutile Particles Following Oral Administration, Sangeetha Aula, Samyuktha Lakkireddy, Atya Kapley, Neha Hebalkar, Rakesh Kumar Sharma, Shantveer G Uppin and Kaiser Jamil, Nano Progress, 2020, 2, 11-20
• Induction of Oxidative Stress, DNA Damage, Hematological and Histopathological Effects of Silica Nanoparticles and Ethanol Solvent in Swiss Albino Mice, Sangeetha Aula, Samyuktha Lakkireddy, Atya Kapley, Rakesh Kumar Sharma, Shantveer G Uppin and Kaiser Jamil, Nano Progress, 2020, 2, 32-39
• Influence of Administration Route and Dose on Biodistribution Profile and Effects of PEG-PLA Nanoparticles in Mice, Sangeetha Aula, Samyuktha Lakkireddy, Atya Kapley, A.V.N. Swamy, Rakesh Kumar Sharma, Shantveer G Uppin, Kaiser Jamil, European Journal of Engineering science and Technology, 2020, 3, 18-40
• Evaluation of biological effects and toxicity of Cetyltrimethyl ammonium bromide stabilized Silver nanoparticles and Cetyltrimethyl ammonium bromide alone following intravenous injection in mice, Sangeetha Aula, Samyuktha Lakkireddy, Atya Kapley, A.V.N. Swamy, Rakesh Kumar Sharma, Shantveer G Uppin, Kaiser Jamil, Current Nanomedicine, 2021, 11, 70-80

• Association of Folate pathway gene polymorphisms in the susceptibility of Chronic Myeloid Leukemia, Recent Advances in Research and Treatment of Human Diseases, Indian Academy of Biomedical Sciences (IABS), Hyderabad, 01/09-01/11/15
• Analysis of CYP1A1 polymorphism in the susceptibility of CML in South Indian population, Emerging trends in Biomarker research- Prospects and new challenges, Jawaharlal Nehru Institute of Advanced Studies (JNIAS), Hyderabad, Telangana, 09/21-09/22/13, Best presentation award
• Risk factors and their implications in the development of Acute Leukemia in children, Environmental Impacts, Health Implications and Therapeutic Approaches (ISEHT- 2012), Vikrama Simhapuri University, Nellore, Andhra Pradesh, 07/15-07/17/12
• Study of Biomarkers in Leukemia, Biomarkers for Diseases- Prospects & Challenges, Jawaharlal Nehru Institute of Advanced Studies (JNIAS), Hyderabad, Telangana, 02/24-02/25/12

Training undergone:

• Three months, Basic techniques of biochemistry, involving various biochemical analyses, RT-PCR, protein determination assays, Department of Microbiology, M. S. University of Baroda, Vadodara, Gujarat, India
• Clinical Research, Clinical Data Management, Oracle clinical, RAVE and basics of Pharmacovigilance
• Base and Advanced SAS