SHARIF SAYYED KARIM
MOLECULAR BIOLOGIST
MUMBAI
Summary
Dedicated and detail-oriented Molecular Biologist with 11 years of experience in a molecular diagnostic laboratory setting. Proficient at conducting a wide range of molecular biology techniques, including PCR, DNA/RNA extraction, qPCR, and data analysis. Dedicated Molecular Biologist with a strong background in the development of molecular diagnostic kits, specializing in nucleic acid extraction and real-time PCR. Experienced Bioinformatician with a proven track record in the development of molecular diagnostic kits through innovative primer and probe design. Proficient in leveraging bioinformatics tools and data analysis to optimize assay performance. Proven expertise in designing, optimizing, and validating molecular biology protocols for diagnostic applications. Proven ability to contribute to research projects, troubleshoot technical issues, and collaborate effectively with multidisciplinary teams. Seeking a challenging position where I can leverage my skills to contribute to the advancement of molecular diagnostics and enhance the development of innovative molecular diagnostics
Overview
11
11
years of professional experience
12
12
years of post-secondary education
Work History
Molecular Biologist
TAQGEN
MUMBAI
06.2016 - Current
- Designed and optimized assays.
- Extracted DNA and genotype samples using SNP technology.
- Facilitated research into genetic causes of disease.
Senior Molecular Biologist
PhD, Amity University
Mumbai
01.2017 - 01.2023
- Led the development and optimization of nucleic acid extraction kits, resulting in pure & high yield extraction efficiency
- Designed and validated real-time PCR kits for the detection & quantification of [HIV
- HBV] through dried blood cards, contributing to the expansion of the company's product portfolio
- Collaborated closely with cross-functional teams, including research and development, quality control, and manufacturing, to ensure seamless kit development and production processes
- Conducted thorough literature reviews and market analyses to identify emerging trends and technologies in molecular diagnostics, informing new product development strategies
- Played a key role in troubleshooting technical issues during kit development, ensuring the delivery of high-quality and reliable diagnostic products
- Povided training and guidance to junior laboratory staff, ensuring adherence to standard operating procedures
- Provided technical support to customers and internal teams, addressing inquiries and troubleshooting issues related to kit performance
- Conducted In silico Anti-HIV Drug inhibition assays
- Assisted in writing and editing research papers for publication in scientific journals
- Participated in scientific conferences and workshops to stay abreast of the latest developments in molecular biology., Topic: in silico and in vitro assessment of natural molecules against HIV integrase
- Projects primarily involves Computer aided Drug discovery, Advanced Molecular biology techniques
- Applied in-silico approaches to identify potential drug candidates for specific disease targets; Pharmacophore Modeling, Virtual Screening: Virtual Screening
- Conducted molecular dynamics simulations and analyzed structural data to assess drug binding stability and interactions
- Molecular Studies of Integrase strand transfer inhibitor INSTI Drug
- Bioassay: In vitro assay and qPCR
- Contributed to the preparation of research reports and publications based on computational drug design studies
- Presented research findings at lab meetings and contributed to the preparation of research manuscript
- Publications: Authored “A computational overview of Integrase strand transfer inhibitor (INSTIs) against emerging and evolving drug-resistant HIV-1 Integrase
- Mutants” has been accepted in Archives of Microbiology (Springer) on 1st
Molecular Biologist
Mumbai
01.2013 - 01.2017
- Assisted in the design and validation of real-time PCR kits for the detection of infectious diseases, demonstrating 100% sensitivity and specificity
- Assisted in the design and validation of real-time PCR kits for the detection of infectious diseases, demonstrating 100% sensitivity and specificity
- Led the bioinformatics team in the design and optimization of primer and probe sequences for molecular diagnostic kits, resulting in 100% sensitivity and specificity
- Utilized bioinformatics tools, such as Primer3, BLAST, and UCSC Genome Browser, to analyze target sequences and design primers and probes for various diagnostic assays
- Conducted in-depth analyses of genomic and transcriptomic data to identify suitable target regions for primer and probe design
- Collaborated with laboratory scientists to troubleshoot and refine assay designs based on experimental results and feedback
- Implemented and maintained bioinformatics databases to organize and store relevant genomic and primer/probe information.
Education
Ph.D - Molecular Biology
Amity University
Mumbai, Maharashtra 01.2017 - 04.2023
M.Tech - Biotechnology and Bioinformatics
Dr D. Y. Patil
01.2008 - 04.2013
Skills
Nucleic acid extraction, real-time PCR, reverseTranscription, gel electrophoresisKit development: Design, optimization, and validation of molecular diagnosticKitsBioinformatics tools: Proficient in using Primer3, BLAST, UCSC GenomeBrowser, and other relevant toolsResearchDesign and execution of experiments, data interpretation, andManuscript preparationBioinformatics tools: Proficient in using molecular modeling tools, dockingAlgorithms, and virtual screening techniquesComputational biology: Experience in molecular dynamics simulations andStructural bioinformaticsDrug discovery: Familiarity with the drug development pipeline andIntegration of computational strategiesCollaboration: Effective communication and collaboration withInterdisciplinary teamsLaboratory management: Equipment calibration, protocol development, andTroubleshootingTeam collaboration: Effective communication and collaboration withMultidisciplinary teamsQuality control: Implementation of rigorous quality control measures toEnsure product reliabilityRegulatory affairs: Preparation of documentation for regulatory submissionsAnd compliance with industry guidelinesAge : 34 yearsGender : MaleFather’s nameDr SBA KarimMother’s Name : Zubeda SayyedMarital Status : MarriedNationality : IndianReligion : Islam
Skilled in genotyping
Accomplishments
- HAFFKINS Institute of Training, Research and Testing, [Mumbai, 2010]
Timeline
Senior Molecular Biologist
PhD, Amity University
01.2017 - 01.2023
Ph.D - Molecular Biology
Amity University
01.2017 - 04.2023
Molecular Biologist
TAQGEN
06.2016 - Current
Molecular Biologist
01.2013 - 01.2017
M.Tech - Biotechnology and Bioinformatics
Dr D. Y. Patil
01.2008 - 04.2013