Shreya Shambuling Shahapur
Hyderabad,Telangana
Overview
2
2
years of professional experience
Work History
Undergraduate Researcher & First Author
Manipal School of Life Sciences, MAHE
Manipal, Karnataka
01.2024 - 06.2024
- Patented research project: AN ANTHOCYANIN-INFUSED STARCH-BASED BIOPOLYMER FILM FOR REAL-TIME FOOD SPOILAGE DETECTION AND METHOD, and has been submitted to the regional Indian Patent Office - Chennai (RefApp. No. 202541040640 filed on April 28, 2025).
- This project aimed to develop an intelligent, eco-friendly food packaging film by integrating anthocyanins extracted from red cabbage into starch-based bioplastics. These bioplastics were designed to act as pH-sensitive indicators for food freshness, making them particularly suitable to detect spoilage in products such as meat and dairy.
- Keywords: Starch, Bioplastic, Glycerol, Anthocyanin, Silver Nanoparticles, pH indicator, Food Packaging
- Key Contributions: Extracted and purified anthocyanins from Red Cabbage (plant source) using ethanol-methanol solvents, rotary evaporation, and HPLC characterization to check purity and identify particular metabolites. Further tested the anthocyanin color response through a pH sensitive assay and estimated the concentration of the extract. Synthesized an optimal and efficient starch-based biopolymer via the solvent casting method, incorporating glycerol as a plasticizer and varying anthocyanin concentrations.
Researcher
Manipal School of Life Sciences, MAHE
Manipal, Karnataka
08.2024 - 06.2025
- Project 2: Anthocyanin-Infused Starch Bioplastics: A Novel Approach for Enhanced Wound Healing via In Vitro and In Vivo Studies.
- This interdisciplinary project explored the development of a multifunctional wound dressing using anthocyanin-loaded starch bioplastics with integrated silver nanoparticles. It aimed to evaluate their efficacy in accelerating wound healing through a combination of antioxidant, anti-inflammatory, and antimicrobial mechanisms.
- Affiliation: Department of Biophysics & Public Health Genomics, MSLS, MAHE
- Mentors: Dr. Nirmal Mazumder (Guide), Dr. Bharath Prasad (Co-guide)
- Key Contributions: Designed and synthesized starch-based bioplastics loaded with anthocyanins and AgNPs, using solvent casting methods. Performed in-depth chemical characterization: FTIR, UV-Vis spectroscopy, HPLC for anthocyanin profiling.
Intern
NTHRYS Biotech Labs
12.2023 - 12.2023
- Company Overview: A Govt. Registered Research Company
- I worked on a project that targeted brain cancer by reactivating the silenced p14ARF gene, crucial for stabilizing p53 and inducing tumor suppression. This project presented a fascinating opportunity to implement processes such as drug dialysis, SDS-PAGE, RT-PCR, Extraction of DNA/RNA from agarose gels, ELISA, agarose gel electrophoresis, PCR primer design, DNA Extraction from Bacteria, Isolation of membrane-soluble proteins, and Gelatin Coomassie Blue Staining.
- A Govt. Registered Research Company
- The project focused on the epigenetic modulation of p14ARF in brain cancer treatment, targeting the brain tissue of glioblastoma-bearing mice, with key objectives to reverse promoter methylation and histone modifications, thereby restoring p14ARF expression.
- Certificate of Internship: This is to certify that Ms. Shreya Shambuling Shahapur pursuing BSc Biotechnology from Manipal School of Life Sciences has done Internship under the field of Biotechnology.
Education
B.Sc. Biotechnology (Honors) - Biotechnology
Manipal School of Life Sciences, Manipal Academy of Higher Education
Manipal, Udupi, Karnataka06.2025
Class XII - undefined
Suchitra Academy International School
01.2021
Class X - undefined
Sadhu Vaswani International School
01.2019
Skills
- Excel
- Jamovi
- Mega
- UCSC Genome Browser
- BLAST
- Alphafold
- Graphpad
- Biorender
- CEU Mass mediator
- Adobe Illustrator
- KEGG
- PDB
- Uniprot
- Origin
- Schrodinger Maestro
- Image J
- TissueQuant Software
- MASSBANK
- Metaboanalyst
- Light microscopy
- Fluorescent microscopy
- PCR
- RT-PCR
- Sanger sequencing
- Microbiological work
- Cell culture work
- Immunoassays
- Nanoparticle synthesis
- Cellular Assays
- Microbial assays
- MIC assay
- Anti-microbial assay
- Drosophila handling
- Rat handling
- Mice handling
- Wound creation
- Euthanizing animal models
- Administering IP
- SC injections
- Western blotting
- Immunoprecipitation
- Histopathology
- H&E staining
- Immunohistochemistry
- Blood biochemistry analysis
- Drug dialysis
- SDS-PAGE
- Extraction of DNA/RNA from agarose gels
- ELISA
- Agarose gel electrophoresis
- PCR primer design
- DNA Extraction from Bacteria
- Isolation of membrane-soluble proteins
- Gelatin Coomassie Blue Staining
- Next-Generation Sequencing (NGS) workflows
- Ion Torrent sequencing
- Library preparation
- Fluorescent-tagged antibody kits
- SEM
- TEM
- XRD
- HPLC
- MASS Spectroscopy
- Flowcytometry
- UTM
- NMR
- UV-vis Spectroscopy
- WTVR tester
- FTIR
- Optical Tensiometers
- Optical tweezers
- AFM
- DSC
- RAMAN spectroscopy
- Confocal Microscopy
- TGA
- Rotary Evaporator
- Vacuum Concentrator
- Microtome
- TECAN
- UV plate reader
Accomplishments
- 1ST prize for research project at ANVESHAN, GOVT of INDIA, National Competition, 2025-02-20, Ramaiah University of Applied Sciences, Bengaluru, India
- 3RD at the International Level, Chitrakara University, 2025-03-10, Chandigarh, India, Cash prize of Rs. 25000/-
Languages
English
Hindi
Telugu
Kannada
Patents
- Method for synthesis of starch-based biopolymer films with silver nanoparticles and Anthocyanin for enhanced wound healing, 2024-06-18, 202441019535
- A METHOD AND COMPOSITION OF A BIODEGRADABLE FACIAL COSMETIC MASK, 2025-05-08, 202541046182
- AN ANTHOCYANIN-INFUSED STARCH-BASED BIOPOLYMER FILM FOR REAL-TIME FOOD SPOILAGE DETECTION AND METHOD, 2025-04-28, 202541040640
- Method for synthesis of starch-based bio materials with curcumin for wound healing, 2024-12-04, 202441019530
Publications
- Anthocyanin-Incorporated Starch Bioplastic: Characterization Study, Shreya Shahapur, Pooja N, Tilottoma Kargupta, Nirmal Mazumder, Frontiers in Optics + Laser Science, 2024-01-01, JD4A.35, https://doi.org/10.1364/fio.2024.jd4a.35
- Spectroscopic Analysis of Curcumin-Infused Starch Biopolymers, Tilottoma Kargupta, Pooja N, Shreya Shahapur, Nirmal Mazumder, Frontiers in Optics + Laser Science, 2024-01-01, JD4A.36, https://doi.org/10.1364/fio.2024.jd4a.36
- Review paper on Mitochondrial Dysfunction in Alzheimer's and Parkinson's Disease, under preparation
- Review paper on Advances in Anthocyanin-Driven Approaches for Effective Wound Healing, under preparation
- Review paper on CAR T cell therapy - immunology-nanoparticles and nano-enzymes, under preparation
Training
- Poster presentation for MCOPS Research Day, MAHE, 2024-09-14, Starch-Based Smart Film Incorporated with Anthocyanin: A Sustainable Food Quality Sensor
- Presented a working model for the International Science Day KMC, MAHE, 2024-11-14, Starch-bioplastic with Anthocyanin: An Eco-Friendly Sensor for Food Quality Monitoring
- Poster presentation for MAHE- Health Sciences- Research Day 2024, 2024-06-08, Mitochondrial Dysfunction in Alzheimer's and Parkinson's Disease
- Poster presentation on a review paper titled 'Mitochondrial Dysfunction in Alzheimer's and Parkinson's Disease', 2024-04-12, MSLS Research Day Symposium
- Attended Workshop on Patent Drafting and Filing, 2024-02-15, OPTICA, MSLS
Summary
Driven and analytical with a passion for uncovering insights and solving complex problems. Well-versed in research methodologies and data analysis tools, capable of synthesizing information and producing actionable results. Committed to contributing meaningful insights and driving impactful outcomes. Offering a solid foundation in critical thinking and analytical skills, eager to learn and develop in a research-focused environment. Contributes ability to quickly grasp new concepts and methodologies, along with strong data analysis and interpretation skills. Ready to use and develop analytical thinking and research methodologies.
Timeline
Researcher
Manipal School of Life Sciences, MAHE
08.2024 - 06.2025
Undergraduate Researcher & First Author
Manipal School of Life Sciences, MAHE
01.2024 - 06.2024
Intern
NTHRYS Biotech Labs
12.2023 - 12.2023
B.Sc. Biotechnology (Honors) - Biotechnology
Manipal School of Life Sciences, Manipal Academy of Higher Education
Class XII - undefined
Suchitra Academy International School
Class X - undefined
Sadhu Vaswani International School
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