Summary

Overview

Work History

Education

Skills

Publications

Honors And Awards

Oral Presentation in National conference

conferences and workshops

Personal Information

Languages

Affiliations

References

Disclaimer

Timeline

Shreyas D

IARI NEW DELHI

Summary

Dynamic researcher with hands-on experience at the Indian Agricultural Research Institute, specializing in host-pathogen interaction studies using Transcriptomic data analysis, CRISPR/Cas9 genome editing and in-silico protein-protein interaction techniques. Successfully identified the susceptible gene for SSR (Sclerotinia stem rot) and its interacting partner in secreted effectors of Sclerotinia sclerotiorum (in silico). Committed in developing research strategies for sustainable agriculture and addressing global hunger challenges.

Overview

years of professional experience

Work History

Research Experience

Indian agricultural research institute

New Delhi

01.2022 - 02.2025

Analyzed transcriptomic datasets and identified and characterized the most probable susceptible gene for Sclerotinia stem rot in Indian mustard.
Performed Brassica juncea infection with Sclerotinia sclerotiorum for the expression analysis and selected validated gene copies for genome editing.
Developed a CRISPR/Cas9 construct for genome editing in Brassica juncea to enhance resistance against Sclerotinia stem rot. Standardized the sgRNA cloning protocol in the laboratory.
Identified probable interacting protein partner of the susceptible gene via in-silico approaches for potential HIGS (Host-Induced Gene Silencing) application.

Education

MSc - Molecular Biology and Biotechnology

Indian Agricultural Research Institute

New Delhi, India

02.2025

BSc - Agricultural Sciences

University of Agriculturual Science

Dharwad, India

08.2022

Skills

Transcriptome data analysis
Genome annotation
Data analysis(R)
Cis-regulatory elements analysis
Domain and motif analysis
Phylogenetic analysis
Gene mining for genome editing
Identification of gene copy numbers in a polyploid genome
Unique primer designing(For individual copies)
sclerotinia scerotiorum culture and maintanence
Detached leaf assay
Stem inoculation and sample collection in research farm
RNA isolation

CDNA synthesis
QRT-PCR
SgRNA design
CRISPR/Cas9-Induced mutant analysis
In-vitro sgRNAefficiency testing
Plasmid isolation
Tissue culture
SgRNA cloning
Protein-protein docking
Homology modelling
Molecular dynamics simulation
Molecular dynamics analysis(RMSD,RMSFSASA, Radius of gyration and MM-PBSA)

Publications

Two publications as the First author is yet to published.

· First paper which includes the Expression analysis and In-silico identification of the potential interacting partner will be communicated soon.

· Publishing of the second paper includes the validation of the identified susceptible gene through genome-editing takes time as the validation need to be done in T0 and T1 generation

Honors And Awards

All India Rank of 88, 2022, Competitive exam Graduate Aptitude Test-Biotechnology GAT-B, organised by Department of Biotechnology-India
All India Rank of 14, 2022, Competitive exam Junior Research Fellow (JRF) Molecular Biology and Biotechnology, organized by Indian Council of Agricultural Research.

Oral Presentation in National conference

Title of presentation- Molecular profiling of the Sclerotinia sclerotiorum - Brassica pathosystem with advanced genomics studies presented at SKAUST-J, 2025

conferences and workshops

Emerging Innovations in Biochemistry and Biotechnology for the Holistic Development of Agriculture, Society for Plant Biochemistry and Biotechnology, SKAUST-J, 2025
International Conference on Biochemical and Biotechnological Approaches for Crop Improvement, IARI, NIPB-ICAR, Society for Plant Biochemistry and Biotechnology, CSIR-NBRI, 2023
Workshop on Fluorescence microscopy, ICAR-IARI, 2023
Workshop on crop health monitoring using drones, IOTECH, 2023

Personal Information

Father's Name: Dharme gowda MJ
Mother's Name: Manjula DH
Gender: Male
Nationality: Indian
Marital Status: Un-married

Languages

English
Kannada
Hindi

Affiliations

Exceptional teaching capabilities - Was involved in guiding the students in practical classes during my research work.
Part-time badminton assistant coach at Turf Club, Chikkamagaluru
Movie enthusiast - Watches every language movies and was involved in short-role in (19-20-21) kannada movie
Travel Explorer- Has explored many Indian places from Kashmir in the north to Rameshwaram in south solo as well as in groups.

References

1. Dr. Navin C Gupta A.R.S., FSAB (Chaiperson)

Senior Scientist

ICAR- National Institute for Plant Biotechnology (NIPB)

( ICAR /DARE, Ministry of Agriculture and Farmer Welfare, Govt. of India )

Office ph.:011-25848783, 25841787, Ext.: 201

Fax :91-11-25843984.

Email: navin.gupta@icar.gov.in,navinbtc@gmail.com

2. Dr. Kishor Gaikwad (Co-chairperson)

Principal Scientist

ICAR- National Institute for Plant Biotechnology (NIPB)

Email: ks.gaikwad@icar.gov.in, kish2012@gmail.com

Phone: 9811462169

3. Dr. Rhitu Rai (Major member)