Summary
Overview
Work History
Education
Skills
Timeline
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Shreyoshree Mukherjee

Shreyoshree Mukherjee

Research Associate (Scientist)
Mysore,Karnataka

Summary

Research Associate with 3 years of experience in antibody engineering, protein expression, and mammalian cell culture. Demonstrated success in developing bispecific antibodies for BBB delivery. Strong interest in therapeutic antibody discovery and translational protein science.

Overview

3
3
years of professional experience

Work History

Project Associate 2

Indian Institute of Science
Bangalore
12.2023 - 05.2024

Involved in the DST-SERB project - Characterization of Trans-Activation Domains (TADs) and nuclear localization signal of zinc finger transcription factor Mxr1p.

Research Associate

Syngene International Limited, Biocon Park
Bangalore
02.2022 - 11.2023
  • Engineered Human Transferrin Receptor (TfR1)-based bispecific antibodies for blood brain barrier (BBB) delivery.
  • Standardized fusion of Iduronate-2-sulfatase (IDS) and sulfatase modifying factor 1 (SUMF1) with Anti-TfR1.
  • Optimized antibody expression and yield using CHO and HEK293T cells Streamlined gene delivery through lipid- and polymer-based transfection methods.

External Consultant

Syngene International Limited, Biocon Park
Bangalore
08.2021 - 01.2022

Optimized CHO cell line culture conditions for recombinant protein production using shake flask, scale-up bioreactors and automated bioreactor systems.

Research Intern

NITTE University
Mangalore
04.2021 - 06.2021

Received training in cell culture and in vitro assays through two projects:

  • Dose-dependent cytotoxicity analyses of Erlotinib Hydrochloride and Anisomycin using 2D cell culture with HepG2 cells.
  • Evaluation of Alginate/Carboxymethylcellulose Scaffolds for Culturing Liver Cancer Spheroids (hands-on training in 3D cell culture, immunofluorescence, cytotoxicity and functional assays).

Education

Master of Science - Biotechnology

Mangalore University
Mangalore, India
04.2001 -

Bachelor of Science - Biochemistry, Microbiology And Biotechnology

University of Mysore
Mysore, India
04.2001 -

Skills

  • Cell Culture & Maintenance: Handled microbial systems including bacterial, yeast, and mammalian cell lines: CHO, HEK, ExpiCHO, HEK293T, Huh7, and HepG2 Experienced with serum-free and suspension cultures for recombinant protein expression

  • Molecular Biology Techniques: Molecular cloning, restriction digestion, RNA isolation, PCR, electrophoresis, western blotting, and site-directed mutagenesis, qRT-PCR and plasmid preparation

  • Protein Engineering & Expression: Bacterial and yeast transformation; Mammalian transient and stable transfections

  • Protein Purification & Characterisation: Ni-NTA affinity chromatography to purify His-tagged proteins, SDS-PAGE

  • Cell-based Assays: MTT and SRB assay

  • In Vivo & Imaging Techniques: Handled C57BL/6J mouse model Experienced in confocal and fluorescence microscopy

  • Data Analysis & Software: GraphPad Prism, Microsoft Excel Basic knowledge of R Familiarity with image analysis software (eg, ImageJ/Fiji) Bioinformatics tools for antibody/protein sequence analysis (eg, SnapGene, BLAST)

Timeline

Project Associate 2

Indian Institute of Science
12.2023 - 05.2024

Research Associate

Syngene International Limited, Biocon Park
02.2022 - 11.2023

External Consultant

Syngene International Limited, Biocon Park
08.2021 - 01.2022

Research Intern

NITTE University
04.2021 - 06.2021

Master of Science - Biotechnology

Mangalore University
04.2001 -

Bachelor of Science - Biochemistry, Microbiology And Biotechnology

University of Mysore
04.2001 -

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Shreyoshree MukherjeeResearch Associate (Scientist)