Shweta SHARMA

Experienced Research Scientist specializing in stem cell biology, molecular biology, and biochemistry. Proficient in advanced molecular techniques including DNA and RNA isolation, site-directed mutagenesis, molecular cloning, protein purification, Western blotting, and mass spectrometry. Skilled in PCR, RT-qPCR, mammalian cell culture, stable cell line development, immunofluorescence, and flow cytometry. Committed to leveraging scientific breakthroughs to advance genetic research and biotechnology.

Molecular Biology & Biochemistry

• Isolation of plasmid and genomic DNA
• Site directed mutagenesis
• Molecular cloning
• PCR
• Electrophoresis (Agarose, Native and Denaturing SDS PAGE)
• Protein expression and purification (Affinity chromatography, Size exclusion chromatography, Ion-exchange chromatography)
• Western blotting
• Protein interaction- immunoprecipitation (IP)
• In vitro Kinase and Epimerase assay
• Cholesterol Assay
• Quantitative and Qualitative measurement of total lipids using Oil Redo

Cell Biology

• Mammalian cell culture (Human Embryonic Kidney 293, L6 rat skeletal muscle and Chinese Hamster Ovary cells)
• In vitro transfection
• Stable cell line generation
• Immunofluorescence and brightfield microscopy
• Flow Cytometry

Cell culture Engineering

• Batch and mock perfusion cultures in shake flasks
• ELISA
• Media formulation

Mass Spectrometry MALDI TOF MS/MS – Sample Preparation

Microbiology

• Bacterial culture
• Transformation
• Biochemical characterization

• Optimizing chaperone removal strategy from over-expressed recombinant proteins: GNE, a case study, Shweta Sharma, Roop Singh Bora, Kulvinder Singh Saini, Ranjana Arya, Book chapter, 2nd Edition of the volume 'Insoluble Proteins' of the 'Methods in Molecular Biology' book series, 2020
• Functional characterization of GNE mutations prevalent in Asian subjects with GNE myopathy, an ultra-rare neuromuscular disorder, Shweta Sharma, Pratibha Chanana, Ravi Bharadwaj, Sudha Bhattacharya, Ranjana Arya, Biochemie, 2022, 10.1016/j.biochi.2022.03.014
• Elucidation of ER stress and UPR pathway in sialic acid deficient cells: pathological relevance to GNEM, Priyanka Chaudhary, Shweta Sharma, Reema Singh, Ranjana Arya, Journal of Cellular Biochemistry, 2021, 10.1002/jcb.30148
• A skeletal muscle cell-based model system for GNE Myopathy, Patent, 202011019014
• Generation and Characterization of a skeletal muscle cell-based model carrying one single Gne allele: Implications in actin dynamics, Shamulailatpam Shreedarshanee devi, Rashmi Yadav, Fluencephila Mashangva, Priyanka Chaudhary, Shweta Sharma, Ranjana Arya, Molecular Neurobiology, 2021, 10.1007/s12035-021-02549-w
• Calcium and Autophagy complexity in GNE mutants of Asian subjects, Shweta Sharma, Fluencephila Mashangva, Jyoti Oswalia, Shagun Singh, Ranjana Arya, Journal of Cellular Physiology, Manuscript submitted, JCP-23-1769

• Gold medalist in B.Sc.
• UGC PG Merit Scholarship Rank Holder
• Qualified All India JNU entrance examination for Ph.D. 2015
• Qualified JNU Combined Entrance Examination for biotechnology programme- M.sc (Agri) (AIR-113)
• Awarded for The Best Poster award in National science day 2019 at JNU

• International Conference on 'Trends in Biochemical and Biomedical Research: Advances and Challenges', Department of Biochemistry, Institute of Science, BHU Varanasi
• National conference on Biological Transactions: From Molecules to Organisms (BTMO-2019), Dept. Of Microbiology and Cell Biology, Indian Institute of Science (IISc), Bangalore
• XLIII All India Cell Biology Conference (AICBC-2019), IISER Mohali, Chandigarh
• Workshop on Intellectual property rights (IPR), CWEI, JNU

1. Dr. Mugdha Gadgil, Scientist

 National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, Maharashtra, 411008,

Phone: +91 20 2590 2433

Mail ID: mc.gadgil@ncl.res.in

2. Dr. Ranjana Arya, Assistant Professor

Jawaharlal Nehru University, New Delhi, Delhi, 110067

Phone: 011-26738754

Mail ID: arya.ranjana24@gmail.com

3. Dr. Debasisa Mohanty, Staff Scientist-VII 

National Institute of Immunology, New Delhi, Delhi, 110067

Phone: +91-9818747627

Mail ID: deb@nii.ac.in