Sri Dharini Sivagiri

• Research Scientist with experience in Molecular Biology Techniques, Biochemical Assays and HPLC techniques. Involved in development of analytical methods for recombinant proteins by conducting thorough literature search. Supervised the work of trainees and interns. Duly maintained the stock of chemicals and consumables.

A. Molecular Biology

• SDS-PAGE (R and NR)
• Western Blotting
• Silver Stain
• qPCR

B. Biochemical Assays

• Bicinchoninic acid assay
• Lowry Assay
• ELISA
• Quantitative analysis of vaccine antigen adsorption to adjuvant by micro-BCA method

C. Commercial Batch Release Tests

• SE-HPLC of MAbs
• UV Spectroscopy
• Residual Host Cell Protein Analysis
• Residual Host Cell DNA Analysis
• Iso-Electric Focusing to identify charge related impurities Residual Protein A Analysis
• Kinetic Turbidimetric assay to measure endotoxin levels in vaccine

D. HPLC Techniques

• Amino Acid Analysis
• SE-HPLC of recombinant proteins

Patent Analyst with experience in conducting comprehensive patent searches using various databases and tools. Analyzed patent documents to identify relevant patents and assessed their impact on current and future projects. Prepared detailed reports summarizing findings and provided strategic insights.

A. Patent Landscape Analysis

• Analyzed and mapped the patent activity within a specific technological area.
• Prepared detailed reports with insights into the emerging trends, key players, technological advancements, and potential areas for innovation to make informed R&D decisions, strategic planning, and intellectual property (IP) portfolio management.

B. Freedom to Operate (FTO) Search

• Performed FTO search in order to to identify any active patents that may restrict the commercialization of the product or service and to determine the risks associated with patent infringement.

C. Patentability Search

• Conducted patentability search to determine if the invention has been disclosed in any of prior art that could impact the novelty of the invention.

D. Technology Scouting

• Performed systematic search for identifying, evaluating, and acquiring external technologies, innovations, or solutions that can be leveraged to enhance the product, services, or overall competitive position of the client company.

E. Sequence based search

• Performed sequence based patent searches and provided similarity report for the target sequence.

F. Clinical Trial Search

• Conducted Clinical trial searches for finding, reviewing, and analyzing clinical trial information from various sources to gather insights into ongoing, completed, or upcoming clinical studies.
• These searches can be valuable for understanding the latest developments in medical research, identifying gaps in treatment options, assessing the competitive landscape in drug development.

• Training was mainly focused on the 'Optimization of Transpeptidation reaction' and the elaborative understanding of the instrumentation and techniques used for the same

• Training was focused on the 'Expression study for proteins synthesized and regulated in bacteria'
• Study involved the use of basic molecular biology techniques like SDS- PAGE, Western blotting, AGE